##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AccIII AclI AflII AhdI AscI
Asp700I AspEI BfrI BsaBI BseAI
BsiEI BsiWI BspDI BspEI Bst1107I
BstEII BstPI BstZ17I ClaI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRV
FseI HpaI MamI MluI MroI
NdeI NheI NotI NruI PacI
PaeR7I PmeI Psp1406I PvuI SalI
SbfI ScaI SfiI SgrAI SmaI
SnaBI SpeI SphI Sse8387I XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 175 GACGTC GACGT^C
Acc65I 1242 GGTACC G^GTACC
AgeI 282 ACCGGT A^CCGGT
AocI 4112 CCTGAGG CC^T[ACGT]AGG
ApaI 2239 GGGCCC GGGCC^C
AseI 2975 ATTAAT AT^TAAT
AsnI 2975 ATTAAT AT^TAAT
Asp718I 1242 GGTACC G^GTACC
AviII 4083 TGCGCA TGC^GCA
BamHI 1787 GGATCC G^GATCC
BclI 542 TGATCA T^GATCA
BlpI 309 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 309 GCTCAGC GC^T[ACGT]AGC
BseRI 139 GAGGAG GAGGAG(10/8)
Bsp1407I 3501 TGTACA T^GTACA
BspHI 1334 TCATGA T^CATGA
BspLU11I 3482 ACATGT A^CATGT
BsrGI 3501 TGTACA T^GTACA
BstAPI 11 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 400 TTCGAA TT^CGAA
Bsu36I 4112 CCTGAGG CC^T[ACGT]AGG
CelII 309 GCTCAGC GC^T[ACGT]AGC
CpoI 210 CGGACCG CG^G[AT]CCG
DrdI 2512 GACCCCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4112 CCTGAGG CC^T[ACGT]AGG
EcoNI 951 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 403 GAATTC G^AATTC
EcoT22I 4453 ATGCAT ATGCA^T
FbaI 542 TGATCA T^GATCA
FspI 4083 TGCGCA TGC^GCA
HincII 2467 GTCAAC GT[CT]^[GA]AC
HindII 2467 GTCAAC GT[CT]^[GA]AC
HindIII 96 AAGCTT A^AGCTT
KpnI 1242 GGTACC GGTAC^C
KspI 369 CCGCGG CCGC^GG
MfeI 4431 CAATTG C^AATTG
MunI 4431 CAATTG C^AATTG
NcoI 2630 CCATGG C^CATGG
NsiI 4453 ATGCAT ATGCA^T
NspV 400 TTCGAA TT^CGAA
PinAI 282 ACCGGT A^CCGGT
PshAI 2358 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2975 ATTAAT AT^TAAT
PvuII 1828 CAGCTG CAG^CTG
RcaI 1334 TCATGA T^CATGA
RsrII 210 CGGACCG CG^G[AT]CCG
SacII 369 CCGCGG CCGC^GG
SapI 591 GAAGAGC GCTCTTC(1/4)
SexAI 977 ACCTGGT A^CC[AT]GGT
SfuI 400 TTCGAA TT^CGAA
SmlI 43 CTTGAG C^T[CT][GA]AG
SspBI 3501 TGTACA T^GTACA
SspI 4023 AATATT AAT^ATT
SwaI 2856 ATTTAAAT ATTT^AAAT
XbaI 101 TCTAGA T^CTAGA
XcmI 2539 CCAGAGAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 286 GTCTAC GT^[AC][GT]AC
4255 GTCTAC
AcsI 403 GAATTC [GA]^AATT[CT]
3339 AAATTT
Alw44I 73 GTGCAC G^TGCAC
1696 GTGCAC
Aor51HI 194 AGCGCT AGC^GCT
1437 AGCGCT
ApaLI 73 GTGCAC G^TGCAC
1696 GTGCAC
ApoI 403 GAATTC [GA]^AATT[CT]
3339 AAATTT
AvaI 153 CCCGAG C^[CT]CG[GA]G
238 CCCGAG
BbeI 334 GGCGCC GGCGC^C
356 GGCGCC
BbrPI 3674 CACGTG CAC^GTG
3688 CACGTG
BbvCI 3836 GCTGAGG CCTCAGC(-5/-2)
3946 GCTGAGG
BcgI 2004 CGAAGGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2678 GCAGGCCTCTCG
BciVI 467 GGATAC GTATCC(6/5)
1281 GGATAC
BglI 218 GCCTGTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2118 GCCCCAGGGGC
BsaWI 282 ACCGGT [AT]^CCGG[AT]
689 ACCGGA
BsgI 1393 CTGCAC GTGCAG(16/14)
2641 GTGCAG
BsmBI 2608 GAGACG CGTCTC(1/5)
3454 GAGACG
BsmI 1494 GAATGC GAATGC(1/-1)
3783 GAATGC
BsoBI 153 CCCGAG C^[CT]CG[GA]G
238 CCCGAG
BsrBI 777 GAGCGG CCGCTC(-3/-3)
3793 GAGCGG
BssHII 158 GCGCGC G^CGCGC
259 GCGCGC
DraI 2857 TTTAAA TTT^AAA
3514 TTTAAA
DraIII 976 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1699 CACAGCGTG
DsaI 369 CCGCGG C^C[GA][CT]GG
2630 CCATGG
Eco47III 194 AGCGCT AGC^GCT
1437 AGCGCT
KasI 334 GGCGCC G^GCGCC
356 GGCGCC
MspA1I 369 CCGCGG C[AC]G^C[GT]G
1828 CAGCTG
NaeI 60 GCCGGC GCC^GGC
115 GCCGGC
NarI 334 GGCGCC GG^CGCC
356 GGCGCC
NgoMIV 60 GCCGGC G^CCGGC
115 GCCGGC
PmaCI 3674 CACGTG CAC^GTG
3688 CACGTG
PmlI 3674 CACGTG CAC^GTG
3688 CACGTG
SfoI 334 GGCGCC GGC^GCC
356 GGCGCC
TfiI 2389 GATTC G^A[AT]TC
2415 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AspHI AspI
AvaII AvrII BalI BanI BanII
BbsI BbvI BcnI BfaI BglII
BlnI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XhoII