################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI Aor51HI AscI AspEI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BsaBI BseAI BsiWI BspDI BspEI BspHI BsrBI BsrDI Bst1107I BstBI BstZ17I ClaI CpoI DraIII DrdI Eam1105I Eco47III FbaI FspI KasI KspI MamI MfeI MluI MroI MunI NarI NdeI NotI NspV PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I StuI SwaI XbaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 328 GTAGAC GT^[AC][GT]AC Alw44I 2553 GTGCAC G^TGCAC ApaI 244 GGGCCC GGGCC^C ApaLI 2553 GTGCAC G^TGCAC AspHI 2553 GTGCAC G[AT]GC[AT]^C AspI 1216 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BlpI 1190 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1190 GCTCAGC GC^T[ACGT]AGC BsaAI 344 CACGTA [CT]AC^GT[GA] BsiEI 46 CGGCCG CG[GA][CT]^CG BsiHKAI 2553 GTGCAC G[AT]GC[AT]^C BsmBI 673 GAGACG CGTCTC(1/5) Bsp1407I 1612 TGTACA T^GTACA BspMI 947 GCAGGT ACCTGC(4/8) BsrGI 1612 TGTACA T^GTACA BssHII 861 GCGCGC G^CGCGC BstEII 959 GGTCACC G^GT[ACGT]ACC BstPI 959 GGTCACC G^GT[ACGT]ACC CelII 1190 GCTCAGC GC^T[ACGT]AGC DraI 3737 TTTAAA TTT^AAA EagI 46 CGGCCG C^GGCCG EclXI 46 CGGCCG C^GGCCG Eco52I 46 CGGCCG C^GGCCG EcoO65I 959 GGTCACC G^GT[ACGT]ACC EcoRV 2975 GATATC GAT^ATC FseI 773 GGCCGGCC GGCCGG^CC HindIII 1008 AAGCTT A^AGCTT HpaI 2968 GTTAAC GTT^AAC NheI 2610 GCTAGC G^CTAGC NruI 975 TCGCGA TCG^CGA PaeR7I 3483 CTCGAG C^TCGAG PflFI 1216 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 3652 GACACGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC ScaI 1454 AGTACT AGT^ACT SmaI 269 CCCGGG CCC^GGG SspBI 1612 TGTACA T^GTACA SspI 3809 AATATT AAT^ATT Tth111I 1216 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 3483 CTCGAG C^TCGAG XmaCI 269 CCCGGG C^CCGGG XmaI 269 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Acc65I 2953 GGTACC G^GTACC 3234 GGTACC AocI 2214 CCTAAGG CC^T[ACGT]AGG 2267 CCTTAGG Asp718I 2953 GGTACC G^GTACC 3234 GGTACC BalI 1691 TGGCCA TGG^CCA 2331 TGGCCA BamHI 152 GGATCC G^GATCC 3325 GGATCC BsmI 1015 GCATTC GAATGC(1/-1) 2935 GCATTC BspLU11I 2638 ACATGT A^CATGT 3819 ACATGT Bsu36I 2214 CCTAAGG CC^T[ACGT]AGG 2267 CCTTAGG DsaI 2334 CCATGG C^C[GA][CT]GG 2923 CCATGG Eco81I 2214 CCTAAGG CC^T[ACGT]AGG 2267 CCTTAGG EcoRI 2918 GAATTC G^AATTC 3690 GAATTC EcoT22I 3156 ATGCAT ATGCA^T 3592 ATGCAT HaeII 1402 AGCGCC [GA]GCGC^[CT] 2819 GGCGCT KpnI 2953 GGTACC GGTAC^C 3234 GGTACC MaeII 345 ACGT A^CGT 2424 ACGT MluNI 1691 TGGCCA TGG^CCA 2331 TGGCCA MscI 1691 TGGCCA TGG^CCA 2331 TGGCCA MslI 2392 CATTAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3089 CATAATAATG NaeI 44 GCCGGC GCC^GGC 774 GCCGGC NcoI 2334 CCATGG C^CATGG 2923 CCATGG NgoMIV 44 GCCGGC G^CCGGC 774 GCCGGC NsiI 3156 ATGCAT ATGCA^T 3592 ATGCAT PpuMI 272 GGGACCC [GA]G^G[AT]CC[CT] 2110 GGGACCC SmlI 1398 CTCAAG C^T[CT][GA]AG 3483 CTCGAG XcmI 2072 CCAACAGCTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2319 CCATCCACTGTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI Asp700I AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PacI PflMI PleI PshBI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI