##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
AhdI        Aor51HI     AscI        AspEI       AviII       
AvrII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BsaBI       BseAI       BsiWI       BspDI       
BspEI       BspHI       BsrBI       BsrDI       Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        DraIII      
DrdI        Eam1105I    Eco47III    FbaI        FspI        
KasI        KspI        MamI        MfeI        MluI        
MroI        MunI        NarI        NdeI        NotI        
NspV        PinAI       PmaCI       PmeI        PmlI        
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    StuI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI          328  GTAGAC               GT^[AC][GT]AC
Alw44I       2553  GTGCAC               G^TGCAC
ApaI          244  GGGCCC               GGGCC^C
ApaLI        2553  GTGCAC               G^TGCAC
AspHI        2553  GTGCAC               G[AT]GC[AT]^C
AspI         1216  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BlpI         1190  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1190  GCTCAGC              GC^T[ACGT]AGC
BsaAI         344  CACGTA               [CT]AC^GT[GA]
BsiEI          46  CGGCCG               CG[GA][CT]^CG
BsiHKAI      2553  GTGCAC               G[AT]GC[AT]^C
BsmBI         673  GAGACG               CGTCTC(1/5)
Bsp1407I     1612  TGTACA               T^GTACA
BspMI         947  GCAGGT               ACCTGC(4/8)
BsrGI        1612  TGTACA               T^GTACA
BssHII        861  GCGCGC               G^CGCGC
BstEII        959  GGTCACC              G^GT[ACGT]ACC
BstPI         959  GGTCACC              G^GT[ACGT]ACC
CelII        1190  GCTCAGC              GC^T[ACGT]AGC
DraI         3737  TTTAAA               TTT^AAA
EagI           46  CGGCCG               C^GGCCG
EclXI          46  CGGCCG               C^GGCCG
Eco52I         46  CGGCCG               C^GGCCG
EcoO65I       959  GGTCACC              G^GT[ACGT]ACC
EcoRV        2975  GATATC               GAT^ATC
FseI          773  GGCCGGCC             GGCCGG^CC
HindIII      1008  AAGCTT               A^AGCTT
HpaI         2968  GTTAAC               GTT^AAC
NheI         2610  GCTAGC               G^CTAGC
NruI          975  TCGCGA               TCG^CGA
PaeR7I       3483  CTCGAG               C^TCGAG
PflFI        1216  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        3652  GACACGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI         1454  AGTACT               AGT^ACT
SmaI          269  CCCGGG               CCC^GGG
SspBI        1612  TGTACA               T^GTACA
SspI         3809  AATATT               AAT^ATT
Tth111I      1216  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         3483  CTCGAG               C^TCGAG
XmaCI         269  CCCGGG               C^CCGGG
XmaI          269  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

Acc65I       2953  GGTACC               G^GTACC
             3234  GGTACC              
AocI         2214  CCTAAGG              CC^T[ACGT]AGG
             2267  CCTTAGG             
Asp718I      2953  GGTACC               G^GTACC
             3234  GGTACC              
BalI         1691  TGGCCA               TGG^CCA
             2331  TGGCCA              
BamHI         152  GGATCC               G^GATCC
             3325  GGATCC              
BsmI         1015  GCATTC               GAATGC(1/-1)
             2935  GCATTC              
BspLU11I     2638  ACATGT               A^CATGT
             3819  ACATGT              
Bsu36I       2214  CCTAAGG              CC^T[ACGT]AGG
             2267  CCTTAGG             
DsaI         2334  CCATGG               C^C[GA][CT]GG
             2923  CCATGG              
Eco81I       2214  CCTAAGG              CC^T[ACGT]AGG
             2267  CCTTAGG             
EcoRI        2918  GAATTC               G^AATTC
             3690  GAATTC              
EcoT22I      3156  ATGCAT               ATGCA^T
             3592  ATGCAT              
HaeII        1402  AGCGCC               [GA]GCGC^[CT]
             2819  GGCGCT              
KpnI         2953  GGTACC               GGTAC^C
             3234  GGTACC              
MaeII         345  ACGT                 A^CGT
             2424  ACGT                
MluNI        1691  TGGCCA               TGG^CCA
             2331  TGGCCA              
MscI         1691  TGGCCA               TGG^CCA
             2331  TGGCCA              
MslI         2392  CATTAATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             3089  CATAATAATG          
NaeI           44  GCCGGC               GCC^GGC
              774  GCCGGC              
NcoI         2334  CCATGG               C^CATGG
             2923  CCATGG              
NgoMIV         44  GCCGGC               G^CCGGC
              774  GCCGGC              
NsiI         3156  ATGCAT               ATGCA^T
             3592  ATGCAT              
PpuMI         272  GGGACCC              [GA]G^G[AT]CC[CT]
             2110  GGGACCC             
SmlI         1398  CTCAAG               C^T[CT][GA]AG
             3483  CTCGAG              
XcmI         2072  CCAACAGCTTTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2319  CCATCCACTGTTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       ApoI        
AseI        AsnI        Asp700I     AvaI        AvaII       
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaHI       
BsaI        BsaJI       BsaWI       BseRI       BsgI        
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrFI       BsrI        BssKI       BssSI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PacI        PflMI       PleI        
PshBI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SphI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       XmnI