##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Aor51HI AscI AspEI AviII
AvrII BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BsaBI BseAI BsiWI BspDI
BspEI BspHI BsrBI BsrDI Bst1107I
BstBI BstZ17I ClaI CpoI DraIII
DrdI Eam1105I Eco47III FbaI FspI
KasI KspI MamI MfeI MluI
MroI MunI NarI NdeI NotI
NspV PinAI PmaCI PmeI PmlI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I StuI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 328 GTAGAC GT^[AC][GT]AC
Alw44I 2553 GTGCAC G^TGCAC
ApaI 244 GGGCCC GGGCC^C
ApaLI 2553 GTGCAC G^TGCAC
AspHI 2553 GTGCAC G[AT]GC[AT]^C
AspI 1216 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BlpI 1190 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1190 GCTCAGC GC^T[ACGT]AGC
BsaAI 344 CACGTA [CT]AC^GT[GA]
BsiEI 46 CGGCCG CG[GA][CT]^CG
BsiHKAI 2553 GTGCAC G[AT]GC[AT]^C
BsmBI 673 GAGACG CGTCTC(1/5)
Bsp1407I 1612 TGTACA T^GTACA
BspMI 947 GCAGGT ACCTGC(4/8)
BsrGI 1612 TGTACA T^GTACA
BssHII 861 GCGCGC G^CGCGC
BstEII 959 GGTCACC G^GT[ACGT]ACC
BstPI 959 GGTCACC G^GT[ACGT]ACC
CelII 1190 GCTCAGC GC^T[ACGT]AGC
DraI 3737 TTTAAA TTT^AAA
EagI 46 CGGCCG C^GGCCG
EclXI 46 CGGCCG C^GGCCG
Eco52I 46 CGGCCG C^GGCCG
EcoO65I 959 GGTCACC G^GT[ACGT]ACC
EcoRV 2975 GATATC GAT^ATC
FseI 773 GGCCGGCC GGCCGG^CC
HindIII 1008 AAGCTT A^AGCTT
HpaI 2968 GTTAAC GTT^AAC
NheI 2610 GCTAGC G^CTAGC
NruI 975 TCGCGA TCG^CGA
PaeR7I 3483 CTCGAG C^TCGAG
PflFI 1216 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3652 GACACGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 1454 AGTACT AGT^ACT
SmaI 269 CCCGGG CCC^GGG
SspBI 1612 TGTACA T^GTACA
SspI 3809 AATATT AAT^ATT
Tth111I 1216 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3483 CTCGAG C^TCGAG
XmaCI 269 CCCGGG C^CCGGG
XmaI 269 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 2953 GGTACC G^GTACC
3234 GGTACC
AocI 2214 CCTAAGG CC^T[ACGT]AGG
2267 CCTTAGG
Asp718I 2953 GGTACC G^GTACC
3234 GGTACC
BalI 1691 TGGCCA TGG^CCA
2331 TGGCCA
BamHI 152 GGATCC G^GATCC
3325 GGATCC
BsmI 1015 GCATTC GAATGC(1/-1)
2935 GCATTC
BspLU11I 2638 ACATGT A^CATGT
3819 ACATGT
Bsu36I 2214 CCTAAGG CC^T[ACGT]AGG
2267 CCTTAGG
DsaI 2334 CCATGG C^C[GA][CT]GG
2923 CCATGG
Eco81I 2214 CCTAAGG CC^T[ACGT]AGG
2267 CCTTAGG
EcoRI 2918 GAATTC G^AATTC
3690 GAATTC
EcoT22I 3156 ATGCAT ATGCA^T
3592 ATGCAT
HaeII 1402 AGCGCC [GA]GCGC^[CT]
2819 GGCGCT
KpnI 2953 GGTACC GGTAC^C
3234 GGTACC
MaeII 345 ACGT A^CGT
2424 ACGT
MluNI 1691 TGGCCA TGG^CCA
2331 TGGCCA
MscI 1691 TGGCCA TGG^CCA
2331 TGGCCA
MslI 2392 CATTAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3089 CATAATAATG
NaeI 44 GCCGGC GCC^GGC
774 GCCGGC
NcoI 2334 CCATGG C^CATGG
2923 CCATGG
NgoMIV 44 GCCGGC G^CCGGC
774 GCCGGC
NsiI 3156 ATGCAT ATGCA^T
3592 ATGCAT
PpuMI 272 GGGACCC [GA]G^G[AT]CC[CT]
2110 GGGACCC
SmlI 1398 CTCAAG C^T[CT][GA]AG
3483 CTCGAG
XcmI 2072 CCAACAGCTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2319 CCATCCACTGTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AseI AsnI Asp700I AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PacI PflMI PleI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI