##################################
# NO CUT ENZYMES ( 205 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BbvI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII
BssKI BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DpnII
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRII
EcoRV EcoT14I EcoT22I FbaI FokI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII HphI KasI
KpnI Ksp632I KspI MaeI MaeII
MamI MboI MfeI MflI MluI
MluNI MroI MscI MslI MspI
MunI MvaI MvnI NaeI NarI
NciI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI ScrFI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
TfiI TseI Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 658 GAATTT [GA]^AATT[CT]
AfaI 434 GTAC GT^AC
AflIII 439 ACATGT A^C[GA][CT]GT
ApaI 129 GGGCCC GGGCC^C
ApoI 658 GAATTT [GA]^AATT[CT]
BpmI 162 CTCCAG CTGGAG(16/14)
BsaI 141 GAGACC GGTCTC(1/5)
BsmFI 95 GTCCC GGGAC(10/14)
BsmI 519 GAATGC GAATGC(1/-1)
BspLU11I 439 ACATGT A^CATGT
BsrDI 446 CATTGC GCAATG(2/0)
BsrI 194 CCAGT ACTGG(1/-1)
DraII 128 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 128 GGGGCCC [GA]G^G[ACGT]CC[CT]
Fnu4HI 71 GCGGC GC^[ACGT]GC
HinfI 112 GAGTC G^A[ACGT]TC
ItaI 71 GCGGC GC^[ACGT]GC
MaeIII 426 GTGAC ^GT[ACGT]AC
MspA1I 165 CAGCTG C[AC]G^C[GT]G
NspI 439 ACATGT [GA]CATG^[CT]
PflMI 164 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 112 GAGTC GAGTC(4/5)
PvuII 165 CAGCTG CAG^CTG
RsaI 434 GTAC GT^AC
SspI 370 AATATT AAT^ATT
TaqI 187 TCGA T^CGA
Tsp45I 426 GTGAC ^GT[GC]AC
TthHB8I 187 TCGA T^CGA
Van91I 164 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AluI 166 AGCT AG^CT
239 AGCT
BanII 81 GAGCCC G[GA]GC[CT]^C
129 GGGCCC
BmyI 81 GAGCCC G[AGT]GC[ACT]^C
129 GGGCCC
Bsp1286I 81 GAGCCC G[AGT]GC[ACT]^C
129 GGGCCC
DraI 395 TTTAAA TTT^AAA
595 TTTAAA
HaeIII 73 GGCC GG^CC
130 GGCC
MwoI 74 GCCCGCAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
78 GCAGAGCCCGC
NlaIII 41 CATG CATG^
440 CATG
Tsp509I 645 AATT ^AATT
659 AATT
TspRI 343 CAGTG CA[GC]TG[ACGT][ACGT]^
430 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AciI BsiYI BslI BsmAI Cac8I
Cfr13I DdeI FauI MboII MnlI
MseI NlaIV Sau96I Tru9I