################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AlwNI Aor51HI ApaI AscI Asp700I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BcnI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII HphI KasI KspI MamI MfeI MflI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I Van91I XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 167 GGTACC G^GTACC AciI 171 CCGC CCGC(-3/-1) Alw44I 291 GTGCAC G^TGCAC AlwI 745 GATCC GGATC(4/5) AocI 901 CCTGAGG CC^T[ACGT]AGG ApaLI 291 GTGCAC G^TGCAC AseI 1151 ATTAAT AT^TAAT AsnI 1151 ATTAAT AT^TAAT Asp718I 167 GGTACC G^GTACC BanII 36 GAGCTC G[GA]GC[CT]^C BbsI 262 GAAGAC GAAGAC(2/6) BciVI 981 GTATCC GTATCC(6/5) BclI 1105 TGATCA T^GATCA BpuAI 262 GAAGAC GAAGAC(2/6) BsmAI 28 GAGAC GTCTC(1/5) BspHI 1183 TCATGA T^CATGA BspMI 876 GCAGGT ACCTGC(4/8) Bsu36I 901 CCTGAGG CC^T[ACGT]AGG EarI 205 GAAGAG CTCTTC(1/4) Eco81I 901 CCTGAGG CC^T[ACGT]AGG FbaI 1105 TGATCA T^GATCA HincII 967 GTTGAC GT[CT]^[GA]AC HindII 967 GTTGAC GT[CT]^[GA]AC KpnI 167 GGTACC GGTAC^C Ksp632I 205 GAAGAG CTCTTC(1/4) MaeII 819 ACGT A^CGT MslI 519 CATTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspI 145 GCATGC [GA]CATG^[CT] PacI 1148 TTAATTAA TTAAT^TAA PshBI 1151 ATTAAT AT^TAAT RcaI 1183 TCATGA T^CATGA SacI 36 GAGCTC GAGCT^C SexAI 599 ACCAGGT A^CC[AT]GGT SphI 145 GCATGC GCATG^C SspI 998 AATATT AAT^ATT XbaI 156 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 870 GAATTT [GA]^AATT[CT] 1206 GAATTT AfaI 168 GTAC GT^AC 582 GTAC ApoI 870 GAATTT [GA]^AATT[CT] 1206 GAATTT BalI 69 TGGCCA TGG^CCA 764 TGGCCA BanI 97 GGCACC G^G[CT][GA]CC 167 GGTACC BbvI 546 GCAGC GCAGC(8/12) 142 GCTGC BlpI 10 GCTTAGC GC^T[ACGT]AGC 1120 GCTTAGC BpmI 136 CTGGAG CTGGAG(16/14) 550 CTGGAG Bpu1102I 10 GCTTAGC GC^T[ACGT]AGC 1120 GCTTAGC BseRI 208 GAGGAG GAGGAG(10/8) 487 GAGGAG BstF5I 498 GGATG GGATG(2/0) 1087 GGATG CelII 10 GCTTAGC GC^T[ACGT]AGC 1120 GCTTAGC DraII 691 GGGACCC [GA]G^G[ACGT]CC[CT] 1010 GGGACCT EaeI 69 TGGCCA [CT]^GGCC[GA] 764 TGGCCA EcoO109I 691 GGGACCC [GA]G^G[ACGT]CC[CT] 1010 GGGACCT EcoT14I 110 CCAAGG C^C[AT][AT]GG 326 CCTTGG EcoT22I 191 ATGCAT ATGCA^T 379 ATGCAT Fnu4HI 142 GCTGC GC^[ACGT]GC 546 GCAGC FokI 498 GGATG GGATG(9/13) 1087 GGATG ItaI 142 GCTGC GC^[ACGT]GC 546 GCAGC MboII 205 GAAGA GAAGA(8/7) 262 GAAGA MluNI 69 TGGCCA TGG^CCA 764 TGGCCA MscI 69 TGGCCA TGG^CCA 764 TGGCCA NsiI 191 ATGCAT ATGCA^T 379 ATGCAT PpuMI 691 GGGACCC [GA]G^G[AT]CC[CT] 1010 GGGACCT RsaI 168 GTAC GT^AC 582 GTAC StyI 110 CCAAGG C^C[AT][AT]GG 326 CCTTGG TseI 142 GCTGC G^C[AT]GC 546 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AluI AspHI AvaII BfaI BmyI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII HaeIII HindIII HinfI MaeI MaeIII MboI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PvuII Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI TthHB8I