##################################
# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
AlwNI Aor51HI ApaI AscI Asp700I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BcnI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HpaI HpaII HphI
KasI KspI MamI MfeI MflI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI RsrII SacII SalI
SapI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Tth111I Van91I XcmI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 167 GGTACC G^GTACC
AciI 171 CCGC CCGC(-3/-1)
Alw44I 291 GTGCAC G^TGCAC
AlwI 745 GATCC GGATC(4/5)
AocI 901 CCTGAGG CC^T[ACGT]AGG
ApaLI 291 GTGCAC G^TGCAC
AseI 1151 ATTAAT AT^TAAT
AsnI 1151 ATTAAT AT^TAAT
Asp718I 167 GGTACC G^GTACC
BanII 36 GAGCTC G[GA]GC[CT]^C
BbsI 262 GAAGAC GAAGAC(2/6)
BciVI 981 GTATCC GTATCC(6/5)
BclI 1105 TGATCA T^GATCA
BpuAI 262 GAAGAC GAAGAC(2/6)
BsmAI 28 GAGAC GTCTC(1/5)
BspHI 1183 TCATGA T^CATGA
BspMI 876 GCAGGT ACCTGC(4/8)
Bsu36I 901 CCTGAGG CC^T[ACGT]AGG
EarI 205 GAAGAG CTCTTC(1/4)
Eco81I 901 CCTGAGG CC^T[ACGT]AGG
FbaI 1105 TGATCA T^GATCA
HincII 967 GTTGAC GT[CT]^[GA]AC
HindII 967 GTTGAC GT[CT]^[GA]AC
KpnI 167 GGTACC GGTAC^C
Ksp632I 205 GAAGAG CTCTTC(1/4)
MaeII 819 ACGT A^CGT
MslI 519 CATTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 145 GCATGC [GA]CATG^[CT]
PacI 1148 TTAATTAA TTAAT^TAA
PshBI 1151 ATTAAT AT^TAAT
RcaI 1183 TCATGA T^CATGA
SacI 36 GAGCTC GAGCT^C
SexAI 599 ACCAGGT A^CC[AT]GGT
SphI 145 GCATGC GCATG^C
SspI 998 AATATT AAT^ATT
XbaI 156 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 870 GAATTT [GA]^AATT[CT]
1206 GAATTT
AfaI 168 GTAC GT^AC
582 GTAC
ApoI 870 GAATTT [GA]^AATT[CT]
1206 GAATTT
BalI 69 TGGCCA TGG^CCA
764 TGGCCA
BanI 97 GGCACC G^G[CT][GA]CC
167 GGTACC
BbvI 546 GCAGC GCAGC(8/12)
142 GCTGC
BlpI 10 GCTTAGC GC^T[ACGT]AGC
1120 GCTTAGC
BpmI 136 CTGGAG CTGGAG(16/14)
550 CTGGAG
Bpu1102I 10 GCTTAGC GC^T[ACGT]AGC
1120 GCTTAGC
BseRI 208 GAGGAG GAGGAG(10/8)
487 GAGGAG
BstF5I 498 GGATG GGATG(2/0)
1087 GGATG
CelII 10 GCTTAGC GC^T[ACGT]AGC
1120 GCTTAGC
DraII 691 GGGACCC [GA]G^G[ACGT]CC[CT]
1010 GGGACCT
EaeI 69 TGGCCA [CT]^GGCC[GA]
764 TGGCCA
EcoO109I 691 GGGACCC [GA]G^G[ACGT]CC[CT]
1010 GGGACCT
EcoT14I 110 CCAAGG C^C[AT][AT]GG
326 CCTTGG
EcoT22I 191 ATGCAT ATGCA^T
379 ATGCAT
Fnu4HI 142 GCTGC GC^[ACGT]GC
546 GCAGC
FokI 498 GGATG GGATG(9/13)
1087 GGATG
ItaI 142 GCTGC GC^[ACGT]GC
546 GCAGC
MboII 205 GAAGA GAAGA(8/7)
262 GAAGA
MluNI 69 TGGCCA TGG^CCA
764 TGGCCA
MscI 69 TGGCCA TGG^CCA
764 TGGCCA
NsiI 191 ATGCAT ATGCA^T
379 ATGCAT
PpuMI 691 GGGACCC [GA]G^G[AT]CC[CT]
1010 GGGACCT
RsaI 168 GTAC GT^AC
582 GTAC
StyI 110 CCAAGG C^C[AT][AT]GG
326 CCTTGG
TseI 142 GCTGC G^C[AT]GC
546 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AluI AspHI AvaII BfaI BmyI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstNI Cac8I
Cfr13I DdeI DpnII EcoRII HaeIII
HindIII HinfI MaeI MaeIII MboI
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PvuII Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
Tru9I Tsp45I Tsp509I TspRI TthHB8I