################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## Acc65I AclI AflII AgeI AlwNI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsgI BsiEI BsiWI BsmI BsoBI BspDI BspHI BspLU11I BsrDI BsrFI BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII EagI EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KasI KpnI MamI MfeI MluI MluNI MscI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI StuI SwaI Tru9I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 1086 GACGTC GACGT^C AcsI 230 GAATTT [GA]^AATT[CT] AflIII 97 ACGTGT A^C[GA][CT]GT AhdI 361 GACGCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 434 GTGCAC G^TGCAC AocI 887 CCTCAGG CC^T[ACGT]AGG Aor51HI 1120 AGCGCT AGC^GCT ApaLI 434 GTGCAC G^TGCAC ApoI 230 GAATTT [GA]^AATT[CT] AspEI 361 GACGCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 1154 GGACC G^G[AT]CC BbvCI 447 GCTGAGG CCTCAGC(-5/-2) BcnI 30 CCCGG CC^[GC]GG BglI 885 GCCCTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 607 CTGGAG CTGGAG(16/14) BsaAI 96 TACGTG [CT]AC^GT[GA] BsmBI 897 CGTCTC CGTCTC(1/5) Bsp1407I 100 TGTACA T^GTACA BspMI 1074 ACCTGC ACCTGC(4/8) BsrGI 100 TGTACA T^GTACA BssHII 169 GCGCGC G^CGCGC BstAPI 1034 GCAGGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1117 CCAAGCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 988 AGATCC [GA]^GATC[CT] Bsu36I 887 CCTCAGG CC^T[ACGT]AGG DrdI 41 GACAATGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 361 GACGCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1120 AGCGCT AGC^GCT Eco81I 887 CCTCAGG CC^T[ACGT]AGG HaeII 1120 AGCGCT [GA]GCGC^[CT] HincII 582 GTCGAC GT[CT]^[GA]AC HindII 582 GTCGAC GT[CT]^[GA]AC MflI 988 AGATCC [GA]^GATC[CT] NciI 30 CCCGG CC^[GC]GG NcoI 1175 CCATGG C^CATGG PleI 595 GAGTC GAGTC(4/5) PvuII 429 CAGCTG CAG^CTG SalI 582 GTCGAC G^TCGAC SapI 348 GAAGAGC GCTCTTC(1/4) SbfI 713 CCTGCAGG CCTGCA^GG SfaNI 143 GATGC GCATC(5/9) Sse8387I 713 CCTGCAGG CCTGCA^GG SspBI 100 TGTACA T^GTACA SspI 544 AATATT AAT^ATT Tsp509I 231 AATT ^AATT XcmI 1207 CCAACTTCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 988 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 582 GTCGAC GT^[AC][GT]AC 702 GTCTAC AcyI 153 GACGCC G[GA]^CG[CT]C 1086 GACGTC ApaI 394 GGGCCC GGGCC^C 892 GGGCCC AspHI 434 GTGCAC G[AT]GC[AT]^C 1080 GAGCAC BanII 394 GGGCCC G[GA]GC[CT]^C 892 GGGCCC BbsI 1058 GAAGAC GAAGAC(2/6) 269 GTCTTC BpuAI 1058 GAAGAC GAAGAC(2/6) 269 GTCTTC BsaHI 153 GACGCC G[GA]^CG[CT]C 1086 GACGTC BsiHKAI 434 GTGCAC G[AT]GC[AT]^C 1080 GAGCAC BsmAI 610 GAGAC GTCTC(1/5) 898 GTCTC BsrBI 70 CCGCTC CCGCTC(-3/-3) 1020 CCGCTC EarI 348 GAAGAG CTCTTC(1/4) 592 GAAGAG EcoT14I 1150 CCTTGG C^C[AT][AT]GG 1175 CCATGG FauI 1094 GCGGG CCCGC(4/6) 1134 GCGGG Hin1I 153 GACGCC G[GA]^CG[CT]C 1086 GACGTC HphI 287 GGTGA GGTGA(8/7) 241 TCACC Ksp632I 348 GAAGAG CTCTTC(1/4) 592 GAAGAG MslI 437 CACAACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 839 CATGATTGTG NlaIV 394 GGGCCC GG[ACGT]^[ACGT]CC 892 GGGCCC StyI 1150 CCTTGG C^C[AT][AT]GG 1175 CCATGG TfiI 626 GAATC G^A[AT]TC 1222 GAATC TspRI 109 CACTG CA[GC]TG[ACGT][ACGT]^ 614 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccII AccIII AciI AfaI AluI AlwI BbvI BfaI BmyI BsaJI BsaWI BseAI BseRI BsiYI BslI BsmFI Bsp1286I BspEI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII ItaI KspI MaeI MaeII MaeIII MboI MboII MnlI MroI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII PstI RsaI SacII Sau3AI Sau96I ScrFI SfcI TaqI TseI Tsp45I TthHB8I