##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
Acc65I AclI AflII AgeI AlwNI
AscI AseI AsnI Asp700I Asp718I
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BsgI
BsiEI BsiWI BsmI BsoBI BspDI
BspHI BspLU11I BsrDI BsrFI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII EagI EclXI Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KpnI MamI MfeI MluI MluNI
MscI MseI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
StuI SwaI Tru9I Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1086 GACGTC GACGT^C
AcsI 230 GAATTT [GA]^AATT[CT]
AflIII 97 ACGTGT A^C[GA][CT]GT
AhdI 361 GACGCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 434 GTGCAC G^TGCAC
AocI 887 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1120 AGCGCT AGC^GCT
ApaLI 434 GTGCAC G^TGCAC
ApoI 230 GAATTT [GA]^AATT[CT]
AspEI 361 GACGCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 1154 GGACC G^G[AT]CC
BbvCI 447 GCTGAGG CCTCAGC(-5/-2)
BcnI 30 CCCGG CC^[GC]GG
BglI 885 GCCCTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 607 CTGGAG CTGGAG(16/14)
BsaAI 96 TACGTG [CT]AC^GT[GA]
BsmBI 897 CGTCTC CGTCTC(1/5)
Bsp1407I 100 TGTACA T^GTACA
BspMI 1074 ACCTGC ACCTGC(4/8)
BsrGI 100 TGTACA T^GTACA
BssHII 169 GCGCGC G^CGCGC
BstAPI 1034 GCAGGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1117 CCAAGCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 988 AGATCC [GA]^GATC[CT]
Bsu36I 887 CCTCAGG CC^T[ACGT]AGG
DrdI 41 GACAATGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 361 GACGCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1120 AGCGCT AGC^GCT
Eco81I 887 CCTCAGG CC^T[ACGT]AGG
HaeII 1120 AGCGCT [GA]GCGC^[CT]
HincII 582 GTCGAC GT[CT]^[GA]AC
HindII 582 GTCGAC GT[CT]^[GA]AC
MflI 988 AGATCC [GA]^GATC[CT]
NciI 30 CCCGG CC^[GC]GG
NcoI 1175 CCATGG C^CATGG
PleI 595 GAGTC GAGTC(4/5)
PvuII 429 CAGCTG CAG^CTG
SalI 582 GTCGAC G^TCGAC
SapI 348 GAAGAGC GCTCTTC(1/4)
SbfI 713 CCTGCAGG CCTGCA^GG
SfaNI 143 GATGC GCATC(5/9)
Sse8387I 713 CCTGCAGG CCTGCA^GG
SspBI 100 TGTACA T^GTACA
SspI 544 AATATT AAT^ATT
Tsp509I 231 AATT ^AATT
XcmI 1207 CCAACTTCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 988 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 582 GTCGAC GT^[AC][GT]AC
702 GTCTAC
AcyI 153 GACGCC G[GA]^CG[CT]C
1086 GACGTC
ApaI 394 GGGCCC GGGCC^C
892 GGGCCC
AspHI 434 GTGCAC G[AT]GC[AT]^C
1080 GAGCAC
BanII 394 GGGCCC G[GA]GC[CT]^C
892 GGGCCC
BbsI 1058 GAAGAC GAAGAC(2/6)
269 GTCTTC
BpuAI 1058 GAAGAC GAAGAC(2/6)
269 GTCTTC
BsaHI 153 GACGCC G[GA]^CG[CT]C
1086 GACGTC
BsiHKAI 434 GTGCAC G[AT]GC[AT]^C
1080 GAGCAC
BsmAI 610 GAGAC GTCTC(1/5)
898 GTCTC
BsrBI 70 CCGCTC CCGCTC(-3/-3)
1020 CCGCTC
EarI 348 GAAGAG CTCTTC(1/4)
592 GAAGAG
EcoT14I 1150 CCTTGG C^C[AT][AT]GG
1175 CCATGG
FauI 1094 GCGGG CCCGC(4/6)
1134 GCGGG
Hin1I 153 GACGCC G[GA]^CG[CT]C
1086 GACGTC
HphI 287 GGTGA GGTGA(8/7)
241 TCACC
Ksp632I 348 GAAGAG CTCTTC(1/4)
592 GAAGAG
MslI 437 CACAACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
839 CATGATTGTG
NlaIV 394 GGGCCC GG[ACGT]^[ACGT]CC
892 GGGCCC
StyI 1150 CCTTGG C^C[AT][AT]GG
1175 CCATGG
TfiI 626 GAATC G^A[AT]TC
1222 GAATC
TspRI 109 CACTG CA[GC]TG[ACGT][ACGT]^
614 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwI BbvI BfaI BmyI BsaJI
BsaWI BseAI BseRI BsiYI BslI
BsmFI Bsp1286I BspEI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII ItaI KspI MaeI
MaeII MaeIII MboI MboII MnlI
MroI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII PstI RsaI
SacII Sau3AI Sau96I ScrFI SfcI
TaqI TseI Tsp45I TthHB8I