################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AflII AgeI AhdI Alw44I AlwI AlwNI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AviII AvrII BalI BamHI BbeI BbrPI BbsI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsgI BsiEI BsiHKAI BsiWI Bsp1407I BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII Cfr10I CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KasI KpnI KspI MaeIII MamI MfeI MflI MluI MluNI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tsp45I Tsp509I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccIII 623 TCCGGA T^CCGGA AcyI 681 GGCGTC G[GA]^CG[CT]C AflIII 767 ACACGT A^C[GA][CT]GT AocI 575 CCTCAGG CC^T[ACGT]AGG Aor51HI 194 AGCGCT AGC^GCT Asp700I 192 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 348 CTCGGG C^[CT]CG[GA]G BanI 262 GGTGCC G^G[CT][GA]CC BbvCI 432 GCTGAGG CCTCAGC(-5/-2) BcgI 603 GCAAAAGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 871 CCCGG CC^[GC]GG BglI 594 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 681 GGCGTC G[GA]^CG[CT]C BsaI 298 GAGACC GGTCTC(1/5) BsaWI 623 TCCGGA [AT]^CCGG[AT] BseAI 623 TCCGGA T^CCGGA BsmBI 694 CGTCTC CGTCTC(1/5) BsoBI 348 CTCGGG C^[CT]CG[GA]G BspDI 419 ATCGAT AT^CGAT BspEI 623 TCCGGA T^CCGGA BsrBI 207 CCGCTC CCGCTC(-3/-3) BsrI 316 CCAGT ACTGG(1/-1) BstAPI 701 GCATTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 575 CCTCAGG CC^T[ACGT]AGG Cac8I 133 GCTGGC GC[ACGT]^[ACGT]GC ClaI 419 ATCGAT AT^CGAT DpnII 116 GATC ^GATC DraI 988 TTTAAA TTT^AAA Eco47III 194 AGCGCT AGC^GCT Eco81I 575 CCTCAGG CC^T[ACGT]AGG FauI 258 GCGGG CCCGC(4/6) HaeII 194 AGCGCT [GA]GCGC^[CT] HgaI 682 GCGTC GACGC(5/10) Hin1I 681 GGCGTC G[GA]^CG[CT]C HincII 268 GTTGAC GT[CT]^[GA]AC HindII 268 GTTGAC GT[CT]^[GA]AC HphI 942 GGTGA GGTGA(8/7) MaeII 769 ACGT A^CGT MboI 116 GATC ^GATC MroI 623 TCCGGA T^CCGGA MslI 768 CACGTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 563 CAGCTG C[AC]G^C[GT]G NciI 871 CCCGG CC^[GC]GG NdeII 116 GATC ^GATC PpuMI 125 AGGACCT [GA]G^G[AT]CC[CT] PvuII 563 CAGCTG CAG^CTG SapI 490 GAAGAGC GCTCTTC(1/4) Sau3AI 116 GATC ^GATC StuI 836 AGGCCT AGG^CCT XmnI 192 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 670 GTAC GT^AC 801 GTAC ApaI 88 GGGCCC GGGCC^C 105 GGGCCC AspI 689 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 875 GACCCTGTC AvaII 126 GGACC G^G[AT]CC 874 GGACC BsmI 859 GAATGC GAATGC(1/-1) 701 GCATTC CfoI 195 GCGC GCG^C 823 GCGC EarI 490 GAAGAG CTCTTC(1/4) 517 GAAGAG EcoT14I 677 CCAAGG C^C[AT][AT]GG 937 CCAAGG HhaI 195 GCGC GCG^C 823 GCGC HinP1I 195 GCGC G^CGC 823 GCGC HinfI 271 GACTC G^A[ACGT]TC 414 GACTC Ksp632I 490 GAAGAG CTCTTC(1/4) 517 GAAGAG PflFI 689 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 875 GACCCTGTC PleI 271 GACTC GAGTC(4/5) 414 GACTC PstI 75 CTGCAG CTGCA^G 250 CTGCAG RsaI 670 GTAC GT^AC 801 GTAC SfcI 75 CTGCAG C^T[GA][CT]AG 250 CTGCAG SmlI 67 CTTGAG C^T[CT][GA]AG 187 CTCAAG StyI 677 CCAAGG C^C[AT][AT]GG 937 CCAAGG Tth111I 689 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 875 GACCCTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AluI BanII BbvI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HpaII ItaI MaeI MboII MnlI MseI MspI MvaI MwoI NlaIII NlaIV Sau96I ScrFI SfaNI TaqI Tru9I TseI TspRI TthHB8I