##################################
# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AlwNI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsgI BsiEI BsiHKAI
BsiWI Bsp1407I BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I CelII Cfr10I
CpoI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KpnI KspI MaeIII MamI MfeI
MflI MluI MluNI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tsp45I
Tsp509I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 623 TCCGGA T^CCGGA
AcyI 681 GGCGTC G[GA]^CG[CT]C
AflIII 767 ACACGT A^C[GA][CT]GT
AocI 575 CCTCAGG CC^T[ACGT]AGG
Aor51HI 194 AGCGCT AGC^GCT
Asp700I 192 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 348 CTCGGG C^[CT]CG[GA]G
BanI 262 GGTGCC G^G[CT][GA]CC
BbvCI 432 GCTGAGG CCTCAGC(-5/-2)
BcgI 603 GCAAAAGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 871 CCCGG CC^[GC]GG
BglI 594 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 681 GGCGTC G[GA]^CG[CT]C
BsaI 298 GAGACC GGTCTC(1/5)
BsaWI 623 TCCGGA [AT]^CCGG[AT]
BseAI 623 TCCGGA T^CCGGA
BsmBI 694 CGTCTC CGTCTC(1/5)
BsoBI 348 CTCGGG C^[CT]CG[GA]G
BspDI 419 ATCGAT AT^CGAT
BspEI 623 TCCGGA T^CCGGA
BsrBI 207 CCGCTC CCGCTC(-3/-3)
BsrI 316 CCAGT ACTGG(1/-1)
BstAPI 701 GCATTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 575 CCTCAGG CC^T[ACGT]AGG
Cac8I 133 GCTGGC GC[ACGT]^[ACGT]GC
ClaI 419 ATCGAT AT^CGAT
DpnII 116 GATC ^GATC
DraI 988 TTTAAA TTT^AAA
Eco47III 194 AGCGCT AGC^GCT
Eco81I 575 CCTCAGG CC^T[ACGT]AGG
FauI 258 GCGGG CCCGC(4/6)
HaeII 194 AGCGCT [GA]GCGC^[CT]
HgaI 682 GCGTC GACGC(5/10)
Hin1I 681 GGCGTC G[GA]^CG[CT]C
HincII 268 GTTGAC GT[CT]^[GA]AC
HindII 268 GTTGAC GT[CT]^[GA]AC
HphI 942 GGTGA GGTGA(8/7)
MaeII 769 ACGT A^CGT
MboI 116 GATC ^GATC
MroI 623 TCCGGA T^CCGGA
MslI 768 CACGTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 563 CAGCTG C[AC]G^C[GT]G
NciI 871 CCCGG CC^[GC]GG
NdeII 116 GATC ^GATC
PpuMI 125 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 563 CAGCTG CAG^CTG
SapI 490 GAAGAGC GCTCTTC(1/4)
Sau3AI 116 GATC ^GATC
StuI 836 AGGCCT AGG^CCT
XmnI 192 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 670 GTAC GT^AC
801 GTAC
ApaI 88 GGGCCC GGGCC^C
105 GGGCCC
AspI 689 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
875 GACCCTGTC
AvaII 126 GGACC G^G[AT]CC
874 GGACC
BsmI 859 GAATGC GAATGC(1/-1)
701 GCATTC
CfoI 195 GCGC GCG^C
823 GCGC
EarI 490 GAAGAG CTCTTC(1/4)
517 GAAGAG
EcoT14I 677 CCAAGG C^C[AT][AT]GG
937 CCAAGG
HhaI 195 GCGC GCG^C
823 GCGC
HinP1I 195 GCGC G^CGC
823 GCGC
HinfI 271 GACTC G^A[ACGT]TC
414 GACTC
Ksp632I 490 GAAGAG CTCTTC(1/4)
517 GAAGAG
PflFI 689 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
875 GACCCTGTC
PleI 271 GACTC GAGTC(4/5)
414 GACTC
PstI 75 CTGCAG CTGCA^G
250 CTGCAG
RsaI 670 GTAC GT^AC
801 GTAC
SfcI 75 CTGCAG C^T[GA][CT]AG
250 CTGCAG
SmlI 67 CTTGAG C^T[CT][GA]AG
187 CTCAAG
StyI 677 CCAAGG C^C[AT][AT]GG
937 CCAAGG
Tth111I 689 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
875 GACCCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI BanII BbvI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstF5I BstNI Cfr13I DdeI DraII
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HpaII ItaI MaeI MboII
MnlI MseI MspI MvaI MwoI
NlaIII NlaIV Sau96I ScrFI SfaNI
TaqI Tru9I TseI TspRI TthHB8I