##################################
# NO CUT ENZYMES ( 174 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FokI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluNI MroI MscI
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TfiI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 151 CGCG CG^CG
Asp700I 525 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 472 GAGCTC G[AT]GC[AT]^C
BanI 233 GGTGCC G^G[CT][GA]CC
BanII 472 GAGCTC G[GA]GC[CT]^C
BbsI 362 GAAGAC GAAGAC(2/6)
BfaI 264 CTAG C^TAG
BglI 236 GCCGGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 472 GAGCTC G[AGT]GC[ACT]^C
BpuAI 362 GAAGAC GAAGAC(2/6)
BsiHKAI 472 GAGCTC G[AT]GC[AT]^C
BsmBI 276 GAGACG CGTCTC(1/5)
Bsp1286I 472 GAGCTC G[AGT]GC[ACT]^C
BsrBI 500 GAGCGG CCGCTC(-3/-3)
BsrFI 236 GCCGGT [GA]^CCGG[CT]
BstUI 151 CGCG CG^CG
BstXI 294 CCACTAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 270 AGATCC [GA]^GATC[CT]
Cfr10I 236 GCCGGT [GA]^CCGG[CT]
DdeI 540 CTGAG C^T[ACGT]AG
EaeI 243 TGGCCG [CT]^GGCC[GA]
FauI 502 GCGGG CCCGC(4/6)
HapII 237 CCGG C^CGG
HincII 305 GTTGAC GT[CT]^[GA]AC
HindII 305 GTTGAC GT[CT]^[GA]AC
HpaII 237 CCGG C^CGG
MaeI 264 CTAG C^TAG
MboII 362 GAAGA GAAGA(8/7)
MflI 270 AGATCC [GA]^GATC[CT]
MluI 150 ACGCGT A^CGCGT
MspA1I 411 CAGCTG C[AC]G^C[GT]G
MspI 237 CCGG C^CGG
MvnI 151 CGCG CG^CG
PvuII 411 CAGCTG CAG^CTG
SacI 472 GAGCTC GAGCT^C
SfcI 388 CTGTAG C^T[GA][CT]AG
StuI 371 AGGCCT AGG^CCT
TaqI 43 TCGA T^CGA
Tsp509I 603 AATT ^AATT
TthHB8I 43 TCGA T^CGA
XhoII 270 AGATCC [GA]^GATC[CT]
XmnI 525 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AciI 135 GCGG CCGC(-3/-1)
502 GCGG
AfaI 34 GTAC GT^AC
154 GTAC
AflIII 150 ACGCGT A^C[GA][CT]GT
425 ACACGT
AlwI 271 GATCC GGATC(4/5)
99 GGATC
AvaII 209 GGACC G^G[AT]CC
291 GGTCC
BbvI 68 GCAGC GCAGC(8/12)
513 GCAGC
BsmAI 276 GAGAC GTCTC(1/5)
217 GTCTC
BsmFI 57 GGGAC GGGAC(10/14)
324 GTCCC
BssKI 84 CCAGG ^CC[ACGT]GG
466 CCAGG
BstNI 84 CCAGG CC^[AT]GG
466 CCAGG
Cac8I 369 GCAGGC GC[ACGT]^[ACGT]GC
502 GCGGGC
Cfr13I 209 GGACC G^G[ACGT]CC
291 GGTCC
DpnII 100 GATC ^GATC
271 GATC
DsaI 24 CCGTGG C^C[GA][CT]GG
165 CCGTGG
EcoRII 84 CCAGG ^CC[AT]GG
466 CCAGG
Fnu4HI 68 GCAGC GC^[ACGT]GC
513 GCAGC
HaeIII 244 GGCC GG^CC
372 GGCC
HphI 444 GGTGA GGTGA(8/7)
469 GGTGA
ItaI 68 GCAGC GC^[ACGT]GC
513 GCAGC
MaeII 279 ACGT A^CGT
427 ACGT
MboI 100 GATC ^GATC
271 GATC
MnlI 41 CCTC CCTC(7/6)
170 GAGG
MseI 260 TTAA T^TAA
569 TTAA
MslI 295 CACTAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
426 CACGTCTGTG
MvaI 84 CCAGG CC^[AT]GG
466 CCAGG
MwoI 236 GCCGGTGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
465 GCCAGGTGAGC
NdeII 100 GATC ^GATC
271 GATC
NlaIV 233 GGTGCC GG[ACGT]^[ACGT]CC
290 GGGTCC
PshAI 210 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
278 GACGTGGGTC
RsaI 34 GTAC GT^AC
154 GTAC
Sau3AI 100 GATC ^GATC
271 GATC
Sau96I 209 GGACC G^G[ACGT]CC
291 GGTCC
ScrFI 84 CCAGG CC^[ACGT]GG
466 CCAGG
Tru9I 260 TTAA T^TAA
569 TTAA
TseI 68 GCAGC G^C[AT]GC
513 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
#########################################
AluI BsaJI BsiYI BslI MaeIII
Tsp45I TspRI