##################################
# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvaII AvrII BamHI BanII BbrPI
BbvCI BcgI BclI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI BstXI Bsu36I CelII ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MluI MroI
MseI MspA1I MunI MvnI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 162 GTATAC GT^[AC][GT]AC
AfaI 463 GTAC GT^AC
AlwI 445 GGATC GGATC(4/5)
AspHI 548 GTGCTC G[AT]GC[AT]^C
AviII 42 TGCGCA TGC^GCA
BalI 666 TGGCCA TGG^CCA
BbeI 287 GGCGCC GGCGC^C
BciVI 5 GGATAC GTATCC(6/5)
BcnI 247 CCCGG CC^[GC]GG
BfaI 555 CTAG C^TAG
BmyI 548 GTGCTC G[AGT]GC[ACT]^C
BsiHKAI 548 GTGCTC G[AT]GC[AT]^C
BsmBI 199 GAGACG CGTCTC(1/5)
Bsp1286I 548 GTGCTC G[AGT]GC[ACT]^C
BspMI 39 ACCTGC ACCTGC(4/8)
BsrDI 234 GCAATG GCAATG(2/0)
Bst1107I 162 GTATAC GTA^TAC
BstF5I 136 GGATG GGATG(2/0)
BstYI 445 GGATCT [GA]^GATC[CT]
BstZ17I 162 GTATAC GTA^TAC
Cac8I 274 GCCGGC GC[ACGT]^[ACGT]GC
Cfr13I 498 GGCCC G^G[ACGT]CC
DraII 497 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 497 AGGCCCT [GA]G^G[ACGT]CC[CT]
FokI 136 GGATG GGATG(9/13)
FspI 42 TGCGCA TGC^GCA
HaeII 287 GGCGCC [GA]GCGC^[CT]
HgaI 201 GACGC GACGC(5/10)
KasI 287 GGCGCC G^GCGCC
MaeI 555 CTAG C^TAG
MaeII 74 ACGT A^CGT
MflI 445 GGATCT [GA]^GATC[CT]
MluNI 666 TGGCCA TGG^CCA
MscI 666 TGGCCA TGG^CCA
NaeI 274 GCCGGC GCC^GGC
NarI 287 GGCGCC GG^CGCC
NciI 247 CCCGG CC^[GC]GG
NgoMIV 274 GCCGGC G^CCGGC
NspI 270 ACATGC [GA]CATG^[CT]
PflMI 472 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 452 CTGCAG CTGCA^G
RsaI 463 GTAC GT^AC
Sau96I 498 GGCCC G^G[ACGT]CC
ScaI 462 AGTACT AGT^ACT
SexAI 185 ACCAGGT A^CC[AT]GGT
SfcI 452 CTGCAG C^T[GA][CT]AG
SfoI 287 GGCGCC GGC^GCC
TaqI 77 TCGA T^CGA
TthHB8I 77 TCGA T^CGA
Van91I 472 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 445 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 86 CCGC CCGC(-3/-1)
313 GCGG
AcyI 201 GACGCC G[GA]^CG[CT]C
287 GGCGCC
BbsI 239 GAAGAC GAAGAC(2/6)
530 GAAGAC
BpuAI 239 GAAGAC GAAGAC(2/6)
530 GAAGAC
BsaHI 201 GACGCC G[GA]^CG[CT]C
287 GGCGCC
BsaJI 246 CCCCGG C^C[ACGT][ACGT]GG
574 CCCAGG
BsrFI 274 GCCGGC [GA]^CCGG[CT]
474 ACCGGC
BsrI 490 ACTGG ACTGG(1/-1)
144 CCAGT
CfoI 43 GCGC GCG^C
288 GCGC
Cfr10I 274 GCCGGC [GA]^CCGG[CT]
474 ACCGGC
DpnII 355 GATC ^GATC
446 GATC
EaeI 276 CGGCCA [CT]^GGCC[GA]
666 TGGCCA
EarI 297 GAAGAG CTCTTC(1/4)
521 GAAGAG
HhaI 43 GCGC GCG^C
288 GCGC
Hin1I 201 GACGCC G[GA]^CG[CT]C
287 GGCGCC
HinP1I 43 GCGC G^CGC
288 GCGC
HinfI 242 GACTC G^A[ACGT]TC
362 GAGTC
HphI 83 TCACC GGTGA(8/7)
108 TCACC
Ksp632I 297 GAAGAG CTCTTC(1/4)
521 GAAGAG
MaeIII 82 GTCAC ^GT[ACGT]AC
624 GTGAC
MboI 355 GATC ^GATC
446 GATC
NdeII 355 GATC ^GATC
446 GATC
PleI 242 GACTC GAGTC(4/5)
362 GAGTC
Sau3AI 355 GATC ^GATC
446 GATC
Tsp45I 82 GTCAC ^GT[GC]AC
624 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AluI BanI BbvI BsiYI BslI
BsmAI BssKI BstNI DdeI EcoRII
Fnu4HI HaeIII HapII HpaII ItaI
MboII MnlI MslI MspI MvaI
MwoI NlaIII NlaIV ScrFI SfaNI
TseI Tsp509I TspRI