################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AvrII BamHI BanII BbrPI BbvCI BcgI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MroI MseI MspA1I MunI MvnI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 162 GTATAC GT^[AC][GT]AC AfaI 463 GTAC GT^AC AlwI 445 GGATC GGATC(4/5) AspHI 548 GTGCTC G[AT]GC[AT]^C AviII 42 TGCGCA TGC^GCA BalI 666 TGGCCA TGG^CCA BbeI 287 GGCGCC GGCGC^C BciVI 5 GGATAC GTATCC(6/5) BcnI 247 CCCGG CC^[GC]GG BfaI 555 CTAG C^TAG BmyI 548 GTGCTC G[AGT]GC[ACT]^C BsiHKAI 548 GTGCTC G[AT]GC[AT]^C BsmBI 199 GAGACG CGTCTC(1/5) Bsp1286I 548 GTGCTC G[AGT]GC[ACT]^C BspMI 39 ACCTGC ACCTGC(4/8) BsrDI 234 GCAATG GCAATG(2/0) Bst1107I 162 GTATAC GTA^TAC BstF5I 136 GGATG GGATG(2/0) BstYI 445 GGATCT [GA]^GATC[CT] BstZ17I 162 GTATAC GTA^TAC Cac8I 274 GCCGGC GC[ACGT]^[ACGT]GC Cfr13I 498 GGCCC G^G[ACGT]CC DraII 497 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO109I 497 AGGCCCT [GA]G^G[ACGT]CC[CT] FokI 136 GGATG GGATG(9/13) FspI 42 TGCGCA TGC^GCA HaeII 287 GGCGCC [GA]GCGC^[CT] HgaI 201 GACGC GACGC(5/10) KasI 287 GGCGCC G^GCGCC MaeI 555 CTAG C^TAG MaeII 74 ACGT A^CGT MflI 445 GGATCT [GA]^GATC[CT] MluNI 666 TGGCCA TGG^CCA MscI 666 TGGCCA TGG^CCA NaeI 274 GCCGGC GCC^GGC NarI 287 GGCGCC GG^CGCC NciI 247 CCCGG CC^[GC]GG NgoMIV 274 GCCGGC G^CCGGC NspI 270 ACATGC [GA]CATG^[CT] PflMI 472 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 452 CTGCAG CTGCA^G RsaI 463 GTAC GT^AC Sau96I 498 GGCCC G^G[ACGT]CC ScaI 462 AGTACT AGT^ACT SexAI 185 ACCAGGT A^CC[AT]GGT SfcI 452 CTGCAG C^T[GA][CT]AG SfoI 287 GGCGCC GGC^GCC TaqI 77 TCGA T^CGA TthHB8I 77 TCGA T^CGA Van91I 472 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 445 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AciI 86 CCGC CCGC(-3/-1) 313 GCGG AcyI 201 GACGCC G[GA]^CG[CT]C 287 GGCGCC BbsI 239 GAAGAC GAAGAC(2/6) 530 GAAGAC BpuAI 239 GAAGAC GAAGAC(2/6) 530 GAAGAC BsaHI 201 GACGCC G[GA]^CG[CT]C 287 GGCGCC BsaJI 246 CCCCGG C^C[ACGT][ACGT]GG 574 CCCAGG BsrFI 274 GCCGGC [GA]^CCGG[CT] 474 ACCGGC BsrI 490 ACTGG ACTGG(1/-1) 144 CCAGT CfoI 43 GCGC GCG^C 288 GCGC Cfr10I 274 GCCGGC [GA]^CCGG[CT] 474 ACCGGC DpnII 355 GATC ^GATC 446 GATC EaeI 276 CGGCCA [CT]^GGCC[GA] 666 TGGCCA EarI 297 GAAGAG CTCTTC(1/4) 521 GAAGAG HhaI 43 GCGC GCG^C 288 GCGC Hin1I 201 GACGCC G[GA]^CG[CT]C 287 GGCGCC HinP1I 43 GCGC G^CGC 288 GCGC HinfI 242 GACTC G^A[ACGT]TC 362 GAGTC HphI 83 TCACC GGTGA(8/7) 108 TCACC Ksp632I 297 GAAGAG CTCTTC(1/4) 521 GAAGAG MaeIII 82 GTCAC ^GT[ACGT]AC 624 GTGAC MboI 355 GATC ^GATC 446 GATC NdeII 355 GATC ^GATC 446 GATC PleI 242 GACTC GAGTC(4/5) 362 GAGTC Sau3AI 355 GATC ^GATC 446 GATC Tsp45I 82 GTCAC ^GT[GC]AC 624 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AluI BanI BbvI BsiYI BslI BsmAI BssKI BstNI DdeI EcoRII Fnu4HI HaeIII HapII HpaII ItaI MboII MnlI MslI MspI MvaI MwoI NlaIII NlaIV ScrFI SfaNI TseI Tsp509I TspRI