##################################
# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BalI BamHI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
ClaI CpoI DraI DraIII EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MseI
MspA1I MunI MvnI NaeI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tru9I Tsp509I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AcyI 538 GGCGCC G[GA]^CG[CT]C
Alw44I 685 GTGCAC G^TGCAC
ApaI 67 GGGCCC GGGCC^C
ApaLI 685 GTGCAC G^TGCAC
AspI 636 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 496 CTCGGG C^[CT]CG[GA]G
AvaII 856 GGTCC G^G[AT]CC
BanI 538 GGCGCC G^G[CT][GA]CC
BbeI 538 GGCGCC GGCGC^C
BbvI 621 GCTGC GCAGC(8/12)
BclI 23 TGATCA T^GATCA
BglII 147 AGATCT A^GATCT
BsaHI 538 GGCGCC G[GA]^CG[CT]C
BseRI 735 GAGGAG GAGGAG(10/8)
BsoBI 496 CTCGGG C^[CT]CG[GA]G
Bsp1407I 325 TGTACA T^GTACA
BsrFI 136 ACCGGC [GA]^CCGG[CT]
BsrGI 325 TGTACA T^GTACA
BstXI 549 CCAGTGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 147 AGATCT [GA]^GATC[CT]
Cfr10I 136 ACCGGC [GA]^CCGG[CT]
DrdI 409 GACTGCTAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 138 CGGCCA [CT]^GGCC[GA]
EcoNI 425 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 803 CCTTGG C^C[AT][AT]GG
FbaI 23 TGATCA T^GATCA
Fnu4HI 621 GCTGC GC^[ACGT]GC
HaeII 538 GGCGCC [GA]GCGC^[CT]
HapII 137 CCGG C^CGG
HgaI 777 GACGC GACGC(5/10)
Hin1I 538 GGCGCC G[GA]^CG[CT]C
HpaII 137 CCGG C^CGG
ItaI 621 GCTGC GC^[ACGT]GC
KasI 538 GGCGCC G^GCGCC
MaeII 11 ACGT A^CGT
MboII 569 GAAGA GAAGA(8/7)
MflI 147 AGATCT [GA]^GATC[CT]
MslI 275 CATCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 137 CCGG C^CGG
NarI 538 GGCGCC GG^CGCC
NlaIII 732 CATG CATG^
PflFI 636 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 218 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 451 GAGCTC GAGCT^C
SfoI 538 GGCGCC GGC^GCC
SspBI 325 TGTACA T^GTACA
StyI 803 CCTTGG C^C[AT][AT]GG
TaqI 239 TCGA T^CGA
TfiI 473 GAATC G^A[AT]TC
TseI 621 GCTGC G^C[AT]GC
Tth111I 636 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 239 TCGA T^CGA
Van91I 218 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 271 CCAACATCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 147 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 594 GTAGAC GT^[AC][GT]AC
767 GTCTAC
AlwNI 256 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
420 CAGTTCCTG
AocI 456 CCTGAGG CC^T[ACGT]AGG
513 CCTGAGG
BanII 67 GGGCCC G[GA]GC[CT]^C
451 GAGCTC
BfaI 414 CTAG C^TAG
936 CTAG
BpmI 156 CTGGAG CTGGAG(16/14)
851 CTGGAG
BsmFI 1 GGGAC GGGAC(10/14)
193 GGGAC
BsrDI 299 CATTGC GCAATG(2/0)
874 GCAATG
BsrI 689 ACTGG ACTGG(1/-1)
549 CCAGT
Bsu36I 456 CCTGAGG CC^T[ACGT]AGG
513 CCTGAGG
DsaI 280 CCGTGG C^C[GA][CT]GG
404 CCACGG
Eco81I 456 CCTGAGG CC^T[ACGT]AGG
513 CCTGAGG
FauI 72 CCCGC CCCGC(4/6)
835 CCCGC
MaeI 414 CTAG C^TAG
936 CTAG
MwoI 214 GCAACCAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
799 GCTACCTTGGC
PleI 236 GAGTC GAGTC(4/5)
879 GAGTC
Tsp45I 42 GTGAC ^GT[GC]AC
88 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AfaI AluI AspHI BmyI
BsaI BsaJI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FokI HaeIII
HhaI HinP1I HinfI HphI MaeIII
MboI MnlI MvaI NdeII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TspRI