################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AflII AflIII AgeI AhdI AlwI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII ClaI CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MseI MspA1I MunI MvnI NaeI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tsp509I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AcyI 538 GGCGCC G[GA]^CG[CT]C Alw44I 685 GTGCAC G^TGCAC ApaI 67 GGGCCC GGGCC^C ApaLI 685 GTGCAC G^TGCAC AspI 636 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 496 CTCGGG C^[CT]CG[GA]G AvaII 856 GGTCC G^G[AT]CC BanI 538 GGCGCC G^G[CT][GA]CC BbeI 538 GGCGCC GGCGC^C BbvI 621 GCTGC GCAGC(8/12) BclI 23 TGATCA T^GATCA BglII 147 AGATCT A^GATCT BsaHI 538 GGCGCC G[GA]^CG[CT]C BseRI 735 GAGGAG GAGGAG(10/8) BsoBI 496 CTCGGG C^[CT]CG[GA]G Bsp1407I 325 TGTACA T^GTACA BsrFI 136 ACCGGC [GA]^CCGG[CT] BsrGI 325 TGTACA T^GTACA BstXI 549 CCAGTGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 147 AGATCT [GA]^GATC[CT] Cfr10I 136 ACCGGC [GA]^CCGG[CT] DrdI 409 GACTGCTAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 138 CGGCCA [CT]^GGCC[GA] EcoNI 425 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 803 CCTTGG C^C[AT][AT]GG FbaI 23 TGATCA T^GATCA Fnu4HI 621 GCTGC GC^[ACGT]GC HaeII 538 GGCGCC [GA]GCGC^[CT] HapII 137 CCGG C^CGG HgaI 777 GACGC GACGC(5/10) Hin1I 538 GGCGCC G[GA]^CG[CT]C HpaII 137 CCGG C^CGG ItaI 621 GCTGC GC^[ACGT]GC KasI 538 GGCGCC G^GCGCC MaeII 11 ACGT A^CGT MboII 569 GAAGA GAAGA(8/7) MflI 147 AGATCT [GA]^GATC[CT] MslI 275 CATCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 137 CCGG C^CGG NarI 538 GGCGCC GG^CGCC NlaIII 732 CATG CATG^ PflFI 636 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 218 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 451 GAGCTC GAGCT^C SfoI 538 GGCGCC GGC^GCC SspBI 325 TGTACA T^GTACA StyI 803 CCTTGG C^C[AT][AT]GG TaqI 239 TCGA T^CGA TfiI 473 GAATC G^A[AT]TC TseI 621 GCTGC G^C[AT]GC Tth111I 636 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 239 TCGA T^CGA Van91I 218 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 271 CCAACATCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 147 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AccI 594 GTAGAC GT^[AC][GT]AC 767 GTCTAC AlwNI 256 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG 420 CAGTTCCTG AocI 456 CCTGAGG CC^T[ACGT]AGG 513 CCTGAGG BanII 67 GGGCCC G[GA]GC[CT]^C 451 GAGCTC BfaI 414 CTAG C^TAG 936 CTAG BpmI 156 CTGGAG CTGGAG(16/14) 851 CTGGAG BsmFI 1 GGGAC GGGAC(10/14) 193 GGGAC BsrDI 299 CATTGC GCAATG(2/0) 874 GCAATG BsrI 689 ACTGG ACTGG(1/-1) 549 CCAGT Bsu36I 456 CCTGAGG CC^T[ACGT]AGG 513 CCTGAGG DsaI 280 CCGTGG C^C[GA][CT]GG 404 CCACGG Eco81I 456 CCTGAGG CC^T[ACGT]AGG 513 CCTGAGG FauI 72 CCCGC CCCGC(4/6) 835 CCCGC MaeI 414 CTAG C^TAG 936 CTAG MwoI 214 GCAACCAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 799 GCTACCTTGGC PleI 236 GAGTC GAGTC(4/5) 879 GAGTC Tsp45I 42 GTGAC ^GT[GC]AC 88 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AfaI AluI AspHI BmyI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII FokI HaeIII HhaI HinP1I HinfI HphI MaeIII MboI MnlI MvaI NdeII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TspRI