################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI AlwNI AocI ApaI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbeI BbrPI BbsI BciVI BfrI BlnI BpuAI BsaAI BsaBI BseAI BsgI BsiWI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DsaI Eam1105I Eco81I EcoO65I EcoRI EcoRV FseI FspI HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MroI MslI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NotI NruI PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfaNI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tsp509I Tth111I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AatII 126 GACGTC GACGT^C AccII 396 CGCG CG^CG AcyI 126 GACGTC G[GA]^CG[CT]C AfaI 738 GTAC GT^AC AflIII 62 ACGTGT A^C[GA][CT]GT Alw44I 457 GTGCAC G^TGCAC ApaLI 457 GTGCAC G^TGCAC Asp700I 369 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 364 CTCGAG C^[CT]CG[GA]G BamHI 489 GGATCC G^GATCC BanII 815 GGGCTC G[GA]GC[CT]^C BbvCI 493 CCTCAGC CCTCAGC(-5/-2) BclI 857 TGATCA T^GATCA BcnI 462 CCCGG CC^[GC]GG BglII 194 AGATCT A^GATCT BpmI 118 CTGGAG CTGGAG(16/14) BsaHI 126 GACGTC G[GA]^CG[CT]C BsaI 103 GAGACC GGTCTC(1/5) BsaWI 634 ACCGGA [AT]^CCGG[AT] BsiEI 508 CGGCCG CG[GA][CT]^CG BsmBI 124 GAGACG CGTCTC(1/5) BsoBI 364 CTCGAG C^[CT]CG[GA]G BspMI 606 ACCTGC ACCTGC(4/8) BstAPI 410 GCAAGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 601 TTCGAA TT^CGAA BstF5I 276 CATCC GGATG(2/0) BstUI 396 CGCG CG^CG DraII 71 AGGACCT [GA]G^G[ACGT]CC[CT] DraIII 469 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 872 GACTGCTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 508 CGGCCG C^GGCCG EclXI 508 CGGCCG C^GGCCG Eco52I 508 CGGCCG C^GGCCG EcoO109I 71 AGGACCT [GA]G^G[ACGT]CC[CT] EcoT22I 37 ATGCAT ATGCA^T FbaI 857 TGATCA T^GATCA FokI 276 CATCC GGATG(9/13) Hin1I 126 GACGTC G[GA]^CG[CT]C HindIII 502 AAGCTT A^AGCTT MseI 927 TTAA T^TAA MvnI 396 CGCG CG^CG NciI 462 CCCGG CC^[GC]GG NheI 780 GCTAGC G^CTAGC NsiI 37 ATGCAT ATGCA^T NspV 601 TTCGAA TT^CGAA PaeR7I 364 CTCGAG C^TCGAG PflMI 468 CCACAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 71 AGGACCT [GA]G^G[AT]CC[CT] PstI 22 CTGCAG CTGCA^G RsaI 738 GTAC GT^AC ScaI 737 AGTACT AGT^ACT SfiI 585 GGCCGAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 601 TTCGAA TT^CGAA SphI 35 GCATGC GCATG^C Tru9I 927 TTAA T^TAA TspRI 152 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 468 CCACAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 364 CTCGAG C^TCGAG XmnI 369 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### Aor51HI 182 AGCGCT AGC^GCT 435 AGCGCT AspHI 343 GTGCTC G[AT]GC[AT]^C 457 GTGCAC BanI 400 GGCACC G^G[CT][GA]CC 513 GGTGCC BcgI 603 CGAACCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 498 GCAGAAGCTTCG BfaI 76 CTAG C^TAG 781 CTAG BglI 586 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 613 GCCAAGAAGGC BsiHKAI 343 GTGCTC G[AT]GC[AT]^C 457 GTGCAC BsmAI 103 GAGAC GTCTC(1/5) 124 GAGAC BsrBI 650 CCGCTC CCGCTC(-3/-3) 822 GAGCGG BsrFI 510 GCCGGT [GA]^CCGG[CT] 665 ACCGGC BstXI 136 CCACCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 479 CCATGATCCTGG BstYI 194 AGATCT [GA]^GATC[CT] 489 GGATCC CfoI 183 GCGC GCG^C 436 GCGC Cfr10I 510 GCCGGT [GA]^CCGG[CT] 665 ACCGGC EarI 730 CTCTTC CTCTTC(1/4) 658 GAAGAG Eco47III 182 AGCGCT AGC^GCT 435 AGCGCT EcoT14I 98 CCAAGG C^C[AT][AT]GG 383 CCAAGG HaeII 182 AGCGCT [GA]GCGC^[CT] 435 AGCGCT HgaI 18 GACGC GACGC(5/10) 394 GACGC HhaI 183 GCGC GCG^C 436 GCGC HinP1I 183 GCGC G^CGC 436 GCGC HphI 570 GGTGA GGTGA(8/7) 130 TCACC Ksp632I 730 CTCTTC CTCTTC(1/4) 658 GAAGAG MaeI 76 CTAG C^TAG 781 CTAG MflI 194 AGATCT [GA]^GATC[CT] 489 GGATCC NspI 35 GCATGC [GA]CATG^[CT] 920 ACATGC SmlI 364 CTCGAG C^T[CT][GA]AG 439 CTCAAG StyI 98 CCAAGG C^C[AT][AT]GG 383 CCAAGG XhoII 194 AGATCT [GA]^GATC[CT] 489 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AluI AlwI AvaII BalI BbvI BlpI BmyI Bpu1102I BsaJI BseRI BsiYI BslI Bsp1286I BsrI BssKI BssSI BstNI Cac8I CelII Cfr13I DdeI DpnII EaeI EcoNI EcoRII FauI Fnu4HI HaeIII HapII HinfI HpaII ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MspI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfcI TaqI TseI Tsp45I TthHB8I