##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI AlwNI AocI
ApaI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BbeI BbrPI BbsI BciVI BfrI
BlnI BpuAI BsaAI BsaBI BseAI
BsgI BsiWI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DsaI Eam1105I Eco81I EcoO65I EcoRI
EcoRV FseI FspI HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MslI MspA1I
MunI NaeI NarI NcoI NdeI
NgoMIV NotI NruI PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfaNI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TfiI Tsp509I
Tth111I XbaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 126 GACGTC GACGT^C
AccII 396 CGCG CG^CG
AcyI 126 GACGTC G[GA]^CG[CT]C
AfaI 738 GTAC GT^AC
AflIII 62 ACGTGT A^C[GA][CT]GT
Alw44I 457 GTGCAC G^TGCAC
ApaLI 457 GTGCAC G^TGCAC
Asp700I 369 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 364 CTCGAG C^[CT]CG[GA]G
BamHI 489 GGATCC G^GATCC
BanII 815 GGGCTC G[GA]GC[CT]^C
BbvCI 493 CCTCAGC CCTCAGC(-5/-2)
BclI 857 TGATCA T^GATCA
BcnI 462 CCCGG CC^[GC]GG
BglII 194 AGATCT A^GATCT
BpmI 118 CTGGAG CTGGAG(16/14)
BsaHI 126 GACGTC G[GA]^CG[CT]C
BsaI 103 GAGACC GGTCTC(1/5)
BsaWI 634 ACCGGA [AT]^CCGG[AT]
BsiEI 508 CGGCCG CG[GA][CT]^CG
BsmBI 124 GAGACG CGTCTC(1/5)
BsoBI 364 CTCGAG C^[CT]CG[GA]G
BspMI 606 ACCTGC ACCTGC(4/8)
BstAPI 410 GCAAGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 601 TTCGAA TT^CGAA
BstF5I 276 CATCC GGATG(2/0)
BstUI 396 CGCG CG^CG
DraII 71 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 469 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 872 GACTGCTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 508 CGGCCG C^GGCCG
EclXI 508 CGGCCG C^GGCCG
Eco52I 508 CGGCCG C^GGCCG
EcoO109I 71 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 37 ATGCAT ATGCA^T
FbaI 857 TGATCA T^GATCA
FokI 276 CATCC GGATG(9/13)
Hin1I 126 GACGTC G[GA]^CG[CT]C
HindIII 502 AAGCTT A^AGCTT
MseI 927 TTAA T^TAA
MvnI 396 CGCG CG^CG
NciI 462 CCCGG CC^[GC]GG
NheI 780 GCTAGC G^CTAGC
NsiI 37 ATGCAT ATGCA^T
NspV 601 TTCGAA TT^CGAA
PaeR7I 364 CTCGAG C^TCGAG
PflMI 468 CCACAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 71 AGGACCT [GA]G^G[AT]CC[CT]
PstI 22 CTGCAG CTGCA^G
RsaI 738 GTAC GT^AC
ScaI 737 AGTACT AGT^ACT
SfiI 585 GGCCGAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 601 TTCGAA TT^CGAA
SphI 35 GCATGC GCATG^C
Tru9I 927 TTAA T^TAA
TspRI 152 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 468 CCACAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 364 CTCGAG C^TCGAG
XmnI 369 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Aor51HI 182 AGCGCT AGC^GCT
435 AGCGCT
AspHI 343 GTGCTC G[AT]GC[AT]^C
457 GTGCAC
BanI 400 GGCACC G^G[CT][GA]CC
513 GGTGCC
BcgI 603 CGAACCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
498 GCAGAAGCTTCG
BfaI 76 CTAG C^TAG
781 CTAG
BglI 586 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
613 GCCAAGAAGGC
BsiHKAI 343 GTGCTC G[AT]GC[AT]^C
457 GTGCAC
BsmAI 103 GAGAC GTCTC(1/5)
124 GAGAC
BsrBI 650 CCGCTC CCGCTC(-3/-3)
822 GAGCGG
BsrFI 510 GCCGGT [GA]^CCGG[CT]
665 ACCGGC
BstXI 136 CCACCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
479 CCATGATCCTGG
BstYI 194 AGATCT [GA]^GATC[CT]
489 GGATCC
CfoI 183 GCGC GCG^C
436 GCGC
Cfr10I 510 GCCGGT [GA]^CCGG[CT]
665 ACCGGC
EarI 730 CTCTTC CTCTTC(1/4)
658 GAAGAG
Eco47III 182 AGCGCT AGC^GCT
435 AGCGCT
EcoT14I 98 CCAAGG C^C[AT][AT]GG
383 CCAAGG
HaeII 182 AGCGCT [GA]GCGC^[CT]
435 AGCGCT
HgaI 18 GACGC GACGC(5/10)
394 GACGC
HhaI 183 GCGC GCG^C
436 GCGC
HinP1I 183 GCGC G^CGC
436 GCGC
HphI 570 GGTGA GGTGA(8/7)
130 TCACC
Ksp632I 730 CTCTTC CTCTTC(1/4)
658 GAAGAG
MaeI 76 CTAG C^TAG
781 CTAG
MflI 194 AGATCT [GA]^GATC[CT]
489 GGATCC
NspI 35 GCATGC [GA]CATG^[CT]
920 ACATGC
SmlI 364 CTCGAG C^T[CT][GA]AG
439 CTCAAG
StyI 98 CCAAGG C^C[AT][AT]GG
383 CCAAGG
XhoII 194 AGATCT [GA]^GATC[CT]
489 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AlwI AvaII BalI
BbvI BlpI BmyI Bpu1102I BsaJI
BseRI BsiYI BslI Bsp1286I BsrI
BssKI BssSI BstNI Cac8I CelII
Cfr13I DdeI DpnII EaeI EcoNI
EcoRII FauI Fnu4HI HaeIII HapII
HinfI HpaII ItaI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfcI TaqI TseI Tsp45I TthHB8I