################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII Acc65I AccIII AgeI ApaI AscI AseI AsnI Asp718I AspI AviII AvrII BalI BbrPI BbvCI BclI BglI BlnI BsaI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI KpnI MluI MluNI MroI MscI NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI RcaI RsrII SalI SapI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI StyI SwaI Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 320 GTCTAC GT^[AC][GT]AC AclI 83 AACGTT AA^CGTT AflII 1178 CTTAAG C^TTAAG AhdI 1412 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 562 AGCGCT AGC^GCT Asp700I 1013 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1412 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 2757 GGATCC G^GATCC BbeI 2455 GGCGCC GGCGC^C BcgI 1741 CGACATGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2400 GTATCC GTATCC(6/5) BfrI 1178 CTTAAG C^TTAAG BglII 336 AGATCT A^GATCT BlpI 1796 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1796 GCTGAGC GC^T[ACGT]AGC BsaBI 860 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1620 TCCGGT [AT]^CCGG[AT] BsiEI 174 CGGCCG CG[GA][CT]^CG BspLU11I 1569 ACATGT A^CATGT BsrFI 1143 ACCGGC [GA]^CCGG[CT] BssSI 497 CTCGTG CACGAG(-5/-1) BstEII 1293 GGTTACC G^GT[ACGT]ACC BstPI 1293 GGTTACC G^GT[ACGT]ACC CelII 1796 GCTGAGC GC^T[ACGT]AGC Cfr10I 1143 ACCGGC [GA]^CCGG[CT] DraII 1395 AGGCCCT [GA]G^G[ACGT]CC[CT] EaeI 174 CGGCCG [CT]^GGCC[GA] EagI 174 CGGCCG C^GGCCG Eam1105I 1412 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 174 CGGCCG C^GGCCG Eco47III 562 AGCGCT AGC^GCT Eco52I 174 CGGCCG C^GGCCG EcoNI 1921 CCTCTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1395 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 1293 GGTTACC G^GT[ACGT]ACC HpaI 2521 GTTAAC GTT^AAC KasI 2455 GGCGCC G^GCGCC MamI 860 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 2455 GGCGCC GG^CGCC NruI 29 TCGCGA TCG^CGA NspI 1569 ACATGT [GA]CATG^[CT] Psp1406I 83 AACGTT AA^CGTT PvuII 869 CAGCTG CAG^CTG SacI 395 GAGCTC GAGCT^C SexAI 599 ACCAGGT A^CC[AT]GGT SfoI 2455 GGCGCC GGC^GCC SmaI 199 CCCGGG CCC^GGG SspI 683 AATATT AAT^ATT StuI 2351 AGGCCT AGG^CCT XcmI 2744 CCAGAAATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 199 CCCGGG C^CCGGG XmaI 199 CCCGGG C^CCGGG XmnI 1013 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcyI 377 GACGCC G[GA]^CG[CT]C 2455 GGCGCC Alw44I 1876 GTGCAC G^TGCAC 2890 GTGCAC ApaLI 1876 GTGCAC G^TGCAC 2890 GTGCAC BanI 1544 GGTGCC G^G[CT][GA]CC 2455 GGCGCC BsaAI 2769 TACGTG [CT]AC^GT[GA] 3004 TACGTG BsaHI 377 GACGCC G[GA]^CG[CT]C 2455 GGCGCC BsgI 2334 GTGCAG GTGCAG(16/14) 2655 GTGCAG BsrDI 1224 CATTGC GCAATG(2/0) 2614 CATTGC BstAPI 667 GCAGTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1943 GCACCAAGTGC BstXI 1755 CCAAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2679 CCAGAGGCATGG DraI 2247 TTTAAA TTT^AAA 2953 TTTAAA EarI 2112 CTCTTC CTCTTC(1/4) 956 GAAGAG Hin1I 377 GACGCC G[GA]^CG[CT]C 2455 GGCGCC Ksp632I 2112 CTCTTC CTCTTC(1/4) 956 GAAGAG KspI 68 CCGCGG CCGC^GG 207 CCGCGG MfeI 822 CAATTG C^AATTG 1098 CAATTG MslI 1810 CACATCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3045 CACTGTGGTG MunI 822 CAATTG C^AATTG 1098 CAATTG SacII 68 CCGCGG CCGC^GG 207 CCGCGG SmlI 1178 CTTAAG C^T[CT][GA]AG 2439 CTTGAG ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraIII DrdI DsaI Eco81I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII