##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccIII AgeI ApaI
AscI AseI AsnI Asp718I AspI
AviII AvrII BalI BbrPI BbvCI
BclI BglI BlnI BsaI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BsrGI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
KpnI MluI MluNI MroI MscI
NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
RcaI RsrII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI StyI
SwaI Tth111I Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 320 GTCTAC GT^[AC][GT]AC
AclI 83 AACGTT AA^CGTT
AflII 1178 CTTAAG C^TTAAG
AhdI 1412 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 562 AGCGCT AGC^GCT
Asp700I 1013 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1412 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2757 GGATCC G^GATCC
BbeI 2455 GGCGCC GGCGC^C
BcgI 1741 CGACATGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2400 GTATCC GTATCC(6/5)
BfrI 1178 CTTAAG C^TTAAG
BglII 336 AGATCT A^GATCT
BlpI 1796 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1796 GCTGAGC GC^T[ACGT]AGC
BsaBI 860 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1620 TCCGGT [AT]^CCGG[AT]
BsiEI 174 CGGCCG CG[GA][CT]^CG
BspLU11I 1569 ACATGT A^CATGT
BsrFI 1143 ACCGGC [GA]^CCGG[CT]
BssSI 497 CTCGTG CACGAG(-5/-1)
BstEII 1293 GGTTACC G^GT[ACGT]ACC
BstPI 1293 GGTTACC G^GT[ACGT]ACC
CelII 1796 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1143 ACCGGC [GA]^CCGG[CT]
DraII 1395 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 174 CGGCCG [CT]^GGCC[GA]
EagI 174 CGGCCG C^GGCCG
Eam1105I 1412 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 174 CGGCCG C^GGCCG
Eco47III 562 AGCGCT AGC^GCT
Eco52I 174 CGGCCG C^GGCCG
EcoNI 1921 CCTCTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1395 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1293 GGTTACC G^GT[ACGT]ACC
HpaI 2521 GTTAAC GTT^AAC
KasI 2455 GGCGCC G^GCGCC
MamI 860 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 2455 GGCGCC GG^CGCC
NruI 29 TCGCGA TCG^CGA
NspI 1569 ACATGT [GA]CATG^[CT]
Psp1406I 83 AACGTT AA^CGTT
PvuII 869 CAGCTG CAG^CTG
SacI 395 GAGCTC GAGCT^C
SexAI 599 ACCAGGT A^CC[AT]GGT
SfoI 2455 GGCGCC GGC^GCC
SmaI 199 CCCGGG CCC^GGG
SspI 683 AATATT AAT^ATT
StuI 2351 AGGCCT AGG^CCT
XcmI 2744 CCAGAAATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 199 CCCGGG C^CCGGG
XmaI 199 CCCGGG C^CCGGG
XmnI 1013 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 377 GACGCC G[GA]^CG[CT]C
2455 GGCGCC
Alw44I 1876 GTGCAC G^TGCAC
2890 GTGCAC
ApaLI 1876 GTGCAC G^TGCAC
2890 GTGCAC
BanI 1544 GGTGCC G^G[CT][GA]CC
2455 GGCGCC
BsaAI 2769 TACGTG [CT]AC^GT[GA]
3004 TACGTG
BsaHI 377 GACGCC G[GA]^CG[CT]C
2455 GGCGCC
BsgI 2334 GTGCAG GTGCAG(16/14)
2655 GTGCAG
BsrDI 1224 CATTGC GCAATG(2/0)
2614 CATTGC
BstAPI 667 GCAGTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1943 GCACCAAGTGC
BstXI 1755 CCAAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2679 CCAGAGGCATGG
DraI 2247 TTTAAA TTT^AAA
2953 TTTAAA
EarI 2112 CTCTTC CTCTTC(1/4)
956 GAAGAG
Hin1I 377 GACGCC G[GA]^CG[CT]C
2455 GGCGCC
Ksp632I 2112 CTCTTC CTCTTC(1/4)
956 GAAGAG
KspI 68 CCGCGG CCGC^GG
207 CCGCGG
MfeI 822 CAATTG C^AATTG
1098 CAATTG
MslI 1810 CACATCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3045 CACTGTGGTG
MunI 822 CAATTG C^AATTG
1098 CAATTG
SacII 68 CCGCGG CCGC^GG
207 CCGCGG
SmlI 1178 CTTAAG C^T[CT][GA]AG
2439 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraIII DrdI DsaI Eco81I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII