################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AflIII AgeI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspI AvrII BamHI BbrPI BcgI BciVI BfrI BglI BglII BlnI BsaBI BsaI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstXI BstZ17I Cfr10I ClaI CpoI DraI DraIII Eco47III EcoRI EcoRV EcoT22I FseI HgaI HindIII KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI ScaI SfiI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI TfiI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AcsI 1685 GAATTT [GA]^AATT[CT] AcyI 142 GGCGCC G[GA]^CG[CT]C AhdI 1117 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1183 GTGCAC G^TGCAC ApaLI 1183 GTGCAC G^TGCAC ApoI 1685 GAATTT [GA]^AATT[CT] AspEI 1117 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 132 TGCGCA TGC^GCA BbeI 142 GGCGCC GGCGC^C BbvCI 370 CCTCAGC CCTCAGC(-5/-2) BfaI 1498 CTAG C^TAG BlpI 924 GCTCAGC GC^T[ACGT]AGC Bpu1102I 924 GCTCAGC GC^T[ACGT]AGC BsaHI 142 GGCGCC G[GA]^CG[CT]C BsaWI 191 ACCGGA [AT]^CCGG[AT] BsiEI 636 CGGCCG CG[GA][CT]^CG BstAPI 842 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 761 GGTCACC G^GT[ACGT]ACC BstPI 761 GGTCACC G^GT[ACGT]ACC CelII 924 GCTCAGC GC^T[ACGT]AGC DrdI 1048 GACAGTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 636 CGGCCG C^GGCCG Eam1105I 1117 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 636 CGGCCG C^GGCCG Eco52I 636 CGGCCG C^GGCCG EcoNI 1384 CCTCAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 761 GGTCACC G^GT[ACGT]ACC FauI 226 GCGGG CCCGC(4/6) FspI 132 TGCGCA TGC^GCA Hin1I 142 GGCGCC G[GA]^CG[CT]C HpaI 1347 GTTAAC GTT^AAC KasI 142 GGCGCC G^GCGCC MaeI 1498 CTAG C^TAG NarI 142 GGCGCC GG^CGCC NdeI 1009 CATATG CA^TATG NspI 851 GCATGC [GA]CATG^[CT] SacI 199 GAGCTC GAGCT^C SbfI 504 CCTGCAGG CCTGCA^GG SexAI 1444 ACCTGGT A^CC[AT]GGT SfoI 142 GGCGCC GGC^GCC SmaI 1233 CCCGGG CCC^GGG SmlI 1314 CTCAAG C^T[CT][GA]AG SphI 851 GCATGC GCATG^C Sse8387I 504 CCTGCAGG CCTGCA^GG TaqI 171 TCGA T^CGA TthHB8I 171 TCGA T^CGA XmaCI 1233 CCCGGG C^CCGGG XmaI 1233 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 49 GTCTAC GT^[AC][GT]AC 1374 GTAGAC AfaI 123 GTAC GT^AC 1779 GTAC AspHI 199 GAGCTC G[AT]GC[AT]^C 1183 GTGCAC AvaI 626 CTCGGG C^[CT]CG[GA]G 1233 CCCGGG BanII 199 GAGCTC G[GA]GC[CT]^C 913 GAGCCC BbvI 832 GCAGC GCAGC(8/12) 1063 GCAGC BclI 1244 TGATCA T^GATCA 1616 TGATCA BsaAI 115 TACGTG [CT]AC^GT[GA] 1414 TACGTG BsgI 1379 CTGCAC GTGCAG(16/14) 1502 GTGCAG BsiHKAI 199 GAGCTC G[AT]GC[AT]^C 1183 GTGCAC BsoBI 626 CTCGGG C^[CT]CG[GA]G 1233 CCCGGG EarI 106 GAAGAG CTCTTC(1/4) 346 GAAGAG FbaI 1244 TGATCA T^GATCA 1616 TGATCA HaeII 142 GGCGCC [GA]GCGC^[CT] 244 AGCGCC HincII 1347 GTTAAC GT[CT]^[GA]AC 1561 GTTGAC HindII 1347 GTTAAC GT[CT]^[GA]AC 1561 GTTGAC Ksp632I 106 GAAGAG CTCTTC(1/4) 346 GAAGAG PflMI 525 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1302 CCAACACTTGG PstI 505 CTGCAG CTGCA^G 754 CTGCAG PvuII 373 CAGCTG CAG^CTG 897 CAGCTG RsaI 123 GTAC GT^AC 1779 GTAC TseI 832 GCAGC G^C[AT]GC 1063 GCAGC Van91I 525 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1302 CCAACACTTGG XcmI 1328 CCACATGGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1771 CCACTACTGTACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AciI AluI AlwI AlwNI AocI AvaII BalI BanI BbsI BcnI BmyI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI Tru9I Tsp45I Tsp509I TspRI XhoII