##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspI AvrII BamHI BbrPI BcgI
BciVI BfrI BglI BglII BlnI
BsaBI BsaI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstXI BstZ17I Cfr10I ClaI CpoI
DraI DraIII Eco47III EcoRI EcoRV
EcoT22I FseI HgaI HindIII KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI TfiI
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AcsI 1685 GAATTT [GA]^AATT[CT]
AcyI 142 GGCGCC G[GA]^CG[CT]C
AhdI 1117 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1183 GTGCAC G^TGCAC
ApaLI 1183 GTGCAC G^TGCAC
ApoI 1685 GAATTT [GA]^AATT[CT]
AspEI 1117 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 132 TGCGCA TGC^GCA
BbeI 142 GGCGCC GGCGC^C
BbvCI 370 CCTCAGC CCTCAGC(-5/-2)
BfaI 1498 CTAG C^TAG
BlpI 924 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 924 GCTCAGC GC^T[ACGT]AGC
BsaHI 142 GGCGCC G[GA]^CG[CT]C
BsaWI 191 ACCGGA [AT]^CCGG[AT]
BsiEI 636 CGGCCG CG[GA][CT]^CG
BstAPI 842 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 761 GGTCACC G^GT[ACGT]ACC
BstPI 761 GGTCACC G^GT[ACGT]ACC
CelII 924 GCTCAGC GC^T[ACGT]AGC
DrdI 1048 GACAGTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 636 CGGCCG C^GGCCG
Eam1105I 1117 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 636 CGGCCG C^GGCCG
Eco52I 636 CGGCCG C^GGCCG
EcoNI 1384 CCTCAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 761 GGTCACC G^GT[ACGT]ACC
FauI 226 GCGGG CCCGC(4/6)
FspI 132 TGCGCA TGC^GCA
Hin1I 142 GGCGCC G[GA]^CG[CT]C
HpaI 1347 GTTAAC GTT^AAC
KasI 142 GGCGCC G^GCGCC
MaeI 1498 CTAG C^TAG
NarI 142 GGCGCC GG^CGCC
NdeI 1009 CATATG CA^TATG
NspI 851 GCATGC [GA]CATG^[CT]
SacI 199 GAGCTC GAGCT^C
SbfI 504 CCTGCAGG CCTGCA^GG
SexAI 1444 ACCTGGT A^CC[AT]GGT
SfoI 142 GGCGCC GGC^GCC
SmaI 1233 CCCGGG CCC^GGG
SmlI 1314 CTCAAG C^T[CT][GA]AG
SphI 851 GCATGC GCATG^C
Sse8387I 504 CCTGCAGG CCTGCA^GG
TaqI 171 TCGA T^CGA
TthHB8I 171 TCGA T^CGA
XmaCI 1233 CCCGGG C^CCGGG
XmaI 1233 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 49 GTCTAC GT^[AC][GT]AC
1374 GTAGAC
AfaI 123 GTAC GT^AC
1779 GTAC
AspHI 199 GAGCTC G[AT]GC[AT]^C
1183 GTGCAC
AvaI 626 CTCGGG C^[CT]CG[GA]G
1233 CCCGGG
BanII 199 GAGCTC G[GA]GC[CT]^C
913 GAGCCC
BbvI 832 GCAGC GCAGC(8/12)
1063 GCAGC
BclI 1244 TGATCA T^GATCA
1616 TGATCA
BsaAI 115 TACGTG [CT]AC^GT[GA]
1414 TACGTG
BsgI 1379 CTGCAC GTGCAG(16/14)
1502 GTGCAG
BsiHKAI 199 GAGCTC G[AT]GC[AT]^C
1183 GTGCAC
BsoBI 626 CTCGGG C^[CT]CG[GA]G
1233 CCCGGG
EarI 106 GAAGAG CTCTTC(1/4)
346 GAAGAG
FbaI 1244 TGATCA T^GATCA
1616 TGATCA
HaeII 142 GGCGCC [GA]GCGC^[CT]
244 AGCGCC
HincII 1347 GTTAAC GT[CT]^[GA]AC
1561 GTTGAC
HindII 1347 GTTAAC GT[CT]^[GA]AC
1561 GTTGAC
Ksp632I 106 GAAGAG CTCTTC(1/4)
346 GAAGAG
PflMI 525 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1302 CCAACACTTGG
PstI 505 CTGCAG CTGCA^G
754 CTGCAG
PvuII 373 CAGCTG CAG^CTG
897 CAGCTG
RsaI 123 GTAC GT^AC
1779 GTAC
TseI 832 GCAGC G^C[AT]GC
1063 GCAGC
Van91I 525 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1302 CCAACACTTGG
XcmI 1328 CCACATGGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1771 CCACTACTGTACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AluI AlwI AlwNI AocI
AvaII BalI BanI BbsI BcnI
BmyI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco81I EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PpuMI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI Tru9I Tsp45I Tsp509I
TspRI XhoII