##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AccI AccIII AclI AgeI
AhdI Alw44I ApaLI AscI Asp718I
AspEI AspI AviII AvrII BamHI
BbsI BciVI BclI BlnI BpuAI
BsaBI BsaI BsaWI BseAI BsiWI
BsmBI BspDI BspEI BspLU11I BsrFI
BssSI Bst1107I BstEII BstPI BstZ17I
Cfr10I ClaI CpoI DraI Eam1105I
EarI EcoNI EcoO65I EcoT22I FbaI
FseI FspI KpnI Ksp632I MamI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PflFI PflMI
PinAI PmeI Psp1406I PvuI RsrII
SalI SapI SbfI ScaI SfiI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I Van91I XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 2438 GACGTC GACGT^C
AflII 425 CTTAAG C^TTAAG
AflIII 680 ACACGT A^C[GA][CT]GT
Aor51HI 584 AGCGCT AGC^GCT
AseI 2705 ATTAAT AT^TAAT
AsnI 2705 ATTAAT AT^TAAT
BanI 141 GGCGCC G^G[CT][GA]CC
BbeI 141 GGCGCC GGCGC^C
BcgI 410 GCAACTTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 425 CTTAAG C^TTAAG
BsmAI 1605 GAGAC GTCTC(1/5)
BsmI 2646 GAATGC GAATGC(1/-1)
BspHI 951 TCATGA T^CATGA
BspMI 1206 ACCTGC ACCTGC(4/8)
BstAPI 1408 GCAAGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1023 TTCGAA TT^CGAA
DrdI 421 GACCCTTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 78 CGGCCG C^GGCCG
EclXI 78 CGGCCG C^GGCCG
Eco47III 584 AGCGCT AGC^GCT
Eco52I 78 CGGCCG C^GGCCG
EcoRI 1753 GAATTC G^AATTC
EcoRV 1886 GATATC GAT^ATC
HincII 1712 GTTAAC GT[CT]^[GA]AC
HindII 1712 GTTAAC GT[CT]^[GA]AC
HpaI 1712 GTTAAC GTT^AAC
KasI 141 GGCGCC G^GCGCC
KspI 215 CCGCGG CCGC^GG
NarI 141 GGCGCC GG^CGCC
NspV 1023 TTCGAA TT^CGAA
PaeR7I 915 CTCGAG C^TCGAG
PshBI 2705 ATTAAT AT^TAAT
RcaI 951 TCATGA T^CATGA
SacI 1624 GAGCTC GAGCT^C
SacII 215 CCGCGG CCGC^GG
SexAI 1089 ACCAGGT A^CC[AT]GGT
SfoI 141 GGCGCC GGC^GCC
SfuI 1023 TTCGAA TT^CGAA
StuI 1257 AGGCCT AGG^CCT
XcmI 1677 CCAGATTCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 915 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 141 GGCGCC G[GA]^CG[CT]C
2438 GACGTC
AfaI 1446 GTAC GT^AC
2125 GTAC
Asp700I 1901 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2008 GAAAGTGTTC
AspHI 517 GAGCAC G[AT]GC[AT]^C
1624 GAGCTC
BbrPI 681 CACGTG CAC^GTG
1303 CACGTG
BbvCI 967 CCTCAGC CCTCAGC(-5/-2)
985 CCTCAGC
BglI 80 GCCGTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2385 GCCTGTCAGGC
BglII 745 AGATCT A^GATCT
801 AGATCT
BlpI 869 GCTGAGC GC^T[ACGT]AGC
944 GCTCAGC
Bpu1102I 869 GCTGAGC GC^T[ACGT]AGC
944 GCTCAGC
BsaAI 681 CACGTG [CT]AC^GT[GA]
1303 CACGTG
BsaHI 141 GGCGCC G[GA]^CG[CT]C
2438 GACGTC
BsiEI 78 CGGCCG CG[GA][CT]^CG
333 CGACCG
BsiHKAI 517 GAGCAC G[AT]GC[AT]^C
1624 GAGCTC
Bsp1407I 1445 TGTACA T^GTACA
2124 TGTACA
BsrGI 1445 TGTACA T^GTACA
2124 TGTACA
BssHII 89 GCGCGC G^CGCGC
298 GCGCGC
CelII 869 GCTGAGC GC^T[ACGT]AGC
944 GCTCAGC
DraIII 1088 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2349 CACTTTGTG
HaeII 141 GGCGCC [GA]GCGC^[CT]
584 AGCGCT
Hin1I 141 GGCGCC G[GA]^CG[CT]C
2438 GACGTC
HindIII 1999 AAGCTT A^AGCTT
2629 AAGCTT
NcoI 383 CCATGG C^CATGG
510 CCATGG
PmaCI 681 CACGTG CAC^GTG
1303 CACGTG
PmlI 681 CACGTG CAC^GTG
1303 CACGTG
PshAI 2319 GACCTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2339 GACCACTGTC
PstI 405 CTGCAG CTGCA^G
1187 CTGCAG
PvuII 56 CAGCTG CAG^CTG
1721 CAGCTG
RsaI 1446 GTAC GT^AC
2125 GTAC
SspBI 1445 TGTACA T^GTACA
2124 TGTACA
XmnI 1901 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2008 GAAAGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
AlwNI AocI ApaI ApoI AvaI
AvaII BalI BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsgI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrBI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI