##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbsI
BbvCI BcgI BciVI BclI BfrI
BlnI BpuAI BsaBI BsaHI BsaWI
BseAI BsiWI BsmBI BspDI BspEI
BsrBI BssHII Bst1107I BstBI BstXI
BstZ17I ClaI CpoI DraI Eam1105I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccII 838 CGCG CG^CG
Alw44I 1378 GTGCAC G^TGCAC
AlwI 1129 GATCC GGATC(4/5)
AlwNI 304 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 160 CCTGAGG CC^T[ACGT]AGG
Aor51HI 501 AGCGCT AGC^GCT
ApaLI 1378 GTGCAC G^TGCAC
Asp700I 624 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 908 CCCGAG C^[CT]CG[GA]G
BbrPI 432 CACGTG CAC^GTG
BcnI 4 CCGGG CC^[GC]GG
BglII 1667 AGATCT A^GATCT
BsaAI 432 CACGTG [CT]AC^GT[GA]
BsiEI 1 CGGCCG CG[GA][CT]^CG
BsmI 1056 GAATGC GAATGC(1/-1)
BsoBI 908 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1143 TGTACA T^GTACA
BspLU11I 1872 ACATGT A^CATGT
BspMI 61 ACCTGC ACCTGC(4/8)
BsrGI 1143 TGTACA T^GTACA
BssSI 424 CACGAG CACGAG(-5/-1)
BstAPI 1275 GCACCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 784 GGTTACC G^GT[ACGT]ACC
BstPI 784 GGTTACC G^GT[ACGT]ACC
BstUI 838 CGCG CG^CG
BstYI 1667 AGATCT [GA]^GATC[CT]
Bsu36I 160 CCTGAGG CC^T[ACGT]AGG
DrdI 1911 GACCCGATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1 CGGCCG C^GGCCG
EclXI 1 CGGCCG C^GGCCG
Eco47III 501 AGCGCT AGC^GCT
Eco52I 1 CGGCCG C^GGCCG
Eco81I 160 CCTGAGG CC^T[ACGT]AGG
EcoNI 1694 CCTATTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 784 GGTTACC G^GT[ACGT]ACC
HincII 1018 GTCAAC GT[CT]^[GA]AC
HindII 1018 GTCAAC GT[CT]^[GA]AC
HphI 1252 TCACC GGTGA(8/7)
MflI 1667 AGATCT [GA]^GATC[CT]
MvnI 838 CGCG CG^CG
NaeI 419 GCCGGC GCC^GGC
NciI 4 CCGGG CC^[GC]GG
NgoMIV 419 GCCGGC G^CCGGC
PflMI 288 CCATCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 432 CACGTG CAC^GTG
PmlI 432 CACGTG CAC^GTG
PvuII 1972 CAGCTG CAG^CTG
SacI 532 GAGCTC GAGCT^C
SapI 1485 GAAGAGC GCTCTTC(1/4)
ScaI 1725 AGTACT AGT^ACT
SexAI 1373 ACCTGGT A^CC[AT]GGT
SfiI 297 GGCCCCACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 87 CTTGAG C^T[CT][GA]AG
SphI 184 GCATGC GCATG^C
SspBI 1143 TGTACA T^GTACA
TaqI 572 TCGA T^CGA
TthHB8I 572 TCGA T^CGA
Van91I 288 CCATCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1083 TCTAGA T^CTAGA
XhoII 1667 AGATCT [GA]^GATC[CT]
XmnI 624 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 985 AAATTC [GA]^AATT[CT]
2007 AAATTT
AflIII 341 ACGTGT A^C[GA][CT]GT
1872 ACATGT
ApaI 657 GGGCCC GGGCC^C
1555 GGGCCC
ApoI 985 AAATTC [GA]^AATT[CT]
2007 AAATTT
BfaI 1084 CTAG C^TAG
1480 CTAG
BlpI 243 GCTGAGC GC^T[ACGT]AGC
1713 GCTGAGC
BpmI 310 CTGGAG CTGGAG(16/14)
472 CTGGAG
Bpu1102I 243 GCTGAGC GC^T[ACGT]AGC
1713 GCTGAGC
BseRI 911 GAGGAG GAGGAG(10/8)
1415 GAGGAG
BspHI 35 TCATGA T^CATGA
734 TCATGA
BsrDI 756 GCAATG GCAATG(2/0)
1318 GCAATG
CelII 243 GCTGAGC GC^T[ACGT]AGC
1713 GCTGAGC
DraIII 1372 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1535 CACACTGTG
MaeI 1084 CTAG C^TAG
1480 CTAG
MaeII 341 ACGT A^CGT
433 ACGT
MseI 1111 TTAA T^TAA
2005 TTAA
NspI 184 GCATGC [GA]CATG^[CT]
1872 ACATGT
PleI 466 GACTC GAGTC(4/5)
808 GACTC
PstI 522 CTGCAG CTGCA^G
1975 CTGCAG
RcaI 35 TCATGA T^CATGA
734 TCATGA
SfaNI 135 GCATC GCATC(5/9)
206 GCATC
Tru9I 1111 TTAA T^TAA
2005 TTAA
Tsp509I 986 AATT ^AATT
2008 AATT
XcmI 507 CCATGTCCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
585 CCAGGACCATGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BalI BanI BanII BbvI BglI
BmyI BsaI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI Ksp632I
MaeIII MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
PpuMI RsaI Sau3AI Sau96I ScrFI
SfcI StuI StyI TfiI TseI
Tsp45I TspRI