################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AatII AccIII AcyI AgeI AhdI AscI AseI AsnI Asp700I AspEI AspI AviII BbeI BbrPI BglII BsaHI BseAI BsiWI BspDI BspEI BsrBI BssHII BstAPI BstBI BstXI ClaI CpoI Eam1105I EcoRI EcoRV FseI FspI Hin1I HpaI KasI KspI MfeI MroI MunI NaeI NarI NcoI NgoMIV NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI PvuI RsrII SacII SbfI ScaI SexAI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 838 GGTACC G^GTACC AflII 1240 CTTAAG C^TTAAG AocI 972 CCTTAGG CC^T[ACGT]AGG ApaI 2178 GGGCCC GGGCC^C Asp718I 838 GGTACC G^GTACC BalI 3885 TGGCCA TGG^CCA BamHI 4041 GGATCC G^GATCC BcgI 3184 GCACAAGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1288 TGATCA T^GATCA BfrI 1240 CTTAAG C^TTAAG BsaBI 707 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 4275 ACCGGA [AT]^CCGG[AT] BsiEI 1824 CGGCCG CG[GA][CT]^CG Bsp1407I 1356 TGTACA T^GTACA BspHI 3690 TCATGA T^CATGA BsrDI 469 GCAATG GCAATG(2/0) BsrFI 3475 GCCGGT [GA]^CCGG[CT] BsrGI 1356 TGTACA T^GTACA Bst1107I 1389 GTATAC GTA^TAC BstEII 3634 GGTGACC G^GT[ACGT]ACC BstPI 3634 GGTGACC G^GT[ACGT]ACC BstZ17I 1389 GTATAC GTA^TAC Bsu36I 972 CCTTAGG CC^T[ACGT]AGG Cfr10I 3475 GCCGGT [GA]^CCGG[CT] DrdI 3203 GACGAAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 2769 CCGTGG C^C[GA][CT]GG EagI 1824 CGGCCG C^GGCCG EclXI 1824 CGGCCG C^GGCCG Eco52I 1824 CGGCCG C^GGCCG Eco81I 972 CCTTAGG CC^T[ACGT]AGG EcoNI 3262 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 3634 GGTGACC G^GT[ACGT]ACC FbaI 1288 TGATCA T^GATCA KpnI 838 GGTACC GGTAC^C MamI 707 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 789 ACGCGT A^CGCGT MluNI 3885 TGGCCA TGG^CCA MscI 3885 TGGCCA TGG^CCA NdeI 1400 CATATG CA^TATG NheI 4371 GCTAGC G^CTAGC PshAI 1078 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 3690 TCATGA T^CATGA SalI 3815 GTCGAC G^TCGAC SfiI 3424 GGCCAAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 3306 CCCGGG CCC^GGG SspBI 1356 TGTACA T^GTACA SspI 1275 AATATT AAT^ATT SwaI 1270 ATTTAAAT ATTT^AAAT XmaCI 3306 CCCGGG C^CCGGG XmaI 3306 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 1389 GTATAC GT^[AC][GT]AC 3815 GTCGAC AclI 936 AACGTT AA^CGTT 2882 AACGTT AcsI 2045 AAATTT [GA]^AATT[CT] 2142 AAATTC Alw44I 2342 GTGCAC G^TGCAC 2670 GTGCAC Aor51HI 2129 AGCGCT AGC^GCT 3996 AGCGCT ApaLI 2342 GTGCAC G^TGCAC 2670 GTGCAC ApoI 2045 AAATTT [GA]^AATT[CT] 2142 AAATTC BanI 838 GGTACC G^G[CT][GA]CC 3521 GGCACC BbsI 1328 GAAGAC GAAGAC(2/6) 1610 GAAGAC BciVI 437 GGATAC GTATCC(6/5) 4192 GGATAC BglI 2987 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3425 GCCAAGCAGGC BpuAI 1328 GAAGAC GAAGAC(2/6) 1610 GAAGAC BsaAI 2375 TACGTG [CT]AC^GT[GA] 2667 TACGTG BsmI 1934 GAATGC GAATGC(1/-1) 4412 GCATTC BssSI 2738 CACGAG CACGAG(-5/-1) 2821 CACGAG DraIII 1371 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG 1559 CACAAGGTG Eco47III 2129 AGCGCT AGC^GCT 3996 AGCGCT EcoT22I 1403 ATGCAT ATGCA^T 4410 ATGCAT HindIII 1237 AAGCTT A^AGCTT 2444 AAGCTT MslI 4307 CACATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 4345 CATACAGATG NsiI 1403 ATGCAT ATGCA^T 4410 ATGCAT PaeR7I 2929 CTCGAG C^TCGAG 3671 CTCGAG PflMI 428 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4183 CCAGCTGGTGG Psp1406I 936 AACGTT AA^CGTT 2882 AACGTT Tsp45I 3635 GTGAC ^GT[GC]AC 4002 GTGAC Van91I 428 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4183 CCAGCTGGTGG XcmI 442 CCAAGCATACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3840 CCAAGAACGACTTGG XhoI 2929 CTCGAG C^TCGAG 3671 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AlwNI AspHI AvaI AvaII AvrII BanII BbvCI BbvI BcnI BfaI BlnI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII