##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccIII AcyI AgeI AhdI
AscI AseI AsnI Asp700I AspEI
AspI AviII BbeI BbrPI BglII
BsaHI BseAI BsiWI BspDI BspEI
BsrBI BssHII BstAPI BstBI BstXI
ClaI CpoI Eam1105I EcoRI EcoRV
FseI FspI Hin1I HpaI KasI
KspI MfeI MroI MunI NaeI
NarI NcoI NgoMIV NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshBI PvuI RsrII
SacII SbfI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 838 GGTACC G^GTACC
AflII 1240 CTTAAG C^TTAAG
AocI 972 CCTTAGG CC^T[ACGT]AGG
ApaI 2178 GGGCCC GGGCC^C
Asp718I 838 GGTACC G^GTACC
BalI 3885 TGGCCA TGG^CCA
BamHI 4041 GGATCC G^GATCC
BcgI 3184 GCACAAGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1288 TGATCA T^GATCA
BfrI 1240 CTTAAG C^TTAAG
BsaBI 707 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4275 ACCGGA [AT]^CCGG[AT]
BsiEI 1824 CGGCCG CG[GA][CT]^CG
Bsp1407I 1356 TGTACA T^GTACA
BspHI 3690 TCATGA T^CATGA
BsrDI 469 GCAATG GCAATG(2/0)
BsrFI 3475 GCCGGT [GA]^CCGG[CT]
BsrGI 1356 TGTACA T^GTACA
Bst1107I 1389 GTATAC GTA^TAC
BstEII 3634 GGTGACC G^GT[ACGT]ACC
BstPI 3634 GGTGACC G^GT[ACGT]ACC
BstZ17I 1389 GTATAC GTA^TAC
Bsu36I 972 CCTTAGG CC^T[ACGT]AGG
Cfr10I 3475 GCCGGT [GA]^CCGG[CT]
DrdI 3203 GACGAAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2769 CCGTGG C^C[GA][CT]GG
EagI 1824 CGGCCG C^GGCCG
EclXI 1824 CGGCCG C^GGCCG
Eco52I 1824 CGGCCG C^GGCCG
Eco81I 972 CCTTAGG CC^T[ACGT]AGG
EcoNI 3262 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3634 GGTGACC G^GT[ACGT]ACC
FbaI 1288 TGATCA T^GATCA
KpnI 838 GGTACC GGTAC^C
MamI 707 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 789 ACGCGT A^CGCGT
MluNI 3885 TGGCCA TGG^CCA
MscI 3885 TGGCCA TGG^CCA
NdeI 1400 CATATG CA^TATG
NheI 4371 GCTAGC G^CTAGC
PshAI 1078 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3690 TCATGA T^CATGA
SalI 3815 GTCGAC G^TCGAC
SfiI 3424 GGCCAAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 3306 CCCGGG CCC^GGG
SspBI 1356 TGTACA T^GTACA
SspI 1275 AATATT AAT^ATT
SwaI 1270 ATTTAAAT ATTT^AAAT
XmaCI 3306 CCCGGG C^CCGGG
XmaI 3306 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 1389 GTATAC GT^[AC][GT]AC
3815 GTCGAC
AclI 936 AACGTT AA^CGTT
2882 AACGTT
AcsI 2045 AAATTT [GA]^AATT[CT]
2142 AAATTC
Alw44I 2342 GTGCAC G^TGCAC
2670 GTGCAC
Aor51HI 2129 AGCGCT AGC^GCT
3996 AGCGCT
ApaLI 2342 GTGCAC G^TGCAC
2670 GTGCAC
ApoI 2045 AAATTT [GA]^AATT[CT]
2142 AAATTC
BanI 838 GGTACC G^G[CT][GA]CC
3521 GGCACC
BbsI 1328 GAAGAC GAAGAC(2/6)
1610 GAAGAC
BciVI 437 GGATAC GTATCC(6/5)
4192 GGATAC
BglI 2987 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3425 GCCAAGCAGGC
BpuAI 1328 GAAGAC GAAGAC(2/6)
1610 GAAGAC
BsaAI 2375 TACGTG [CT]AC^GT[GA]
2667 TACGTG
BsmI 1934 GAATGC GAATGC(1/-1)
4412 GCATTC
BssSI 2738 CACGAG CACGAG(-5/-1)
2821 CACGAG
DraIII 1371 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1559 CACAAGGTG
Eco47III 2129 AGCGCT AGC^GCT
3996 AGCGCT
EcoT22I 1403 ATGCAT ATGCA^T
4410 ATGCAT
HindIII 1237 AAGCTT A^AGCTT
2444 AAGCTT
MslI 4307 CACATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4345 CATACAGATG
NsiI 1403 ATGCAT ATGCA^T
4410 ATGCAT
PaeR7I 2929 CTCGAG C^TCGAG
3671 CTCGAG
PflMI 428 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4183 CCAGCTGGTGG
Psp1406I 936 AACGTT AA^CGTT
2882 AACGTT
Tsp45I 3635 GTGAC ^GT[GC]AC
4002 GTGAC
Van91I 428 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4183 CCAGCTGGTGG
XcmI 442 CCAAGCATACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3840 CCAAGAACGACTTGG
XhoI 2929 CTCGAG C^TCGAG
3671 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AspHI AvaI AvaII
AvrII BanII BbvCI BbvI BcnI
BfaI BlnI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspLU11I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII