##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I AocI ApaLI
AscI AseI AsnI Asp718I AvrII
BamHI BbeI BbrPI BfrI BlnI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BspHI BspLU11I BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI DraI EagI EclXI Eco52I
Eco81I EcoO65I EcoRV EcoT22I FseI
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 2410 GGCGTC G[GA]^CG[CT]C
AflIII 1804 ACGTGT A^C[GA][CT]GT
AhdI 1783 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1199 AGCGCT AGC^GCT
ApaI 2500 GGGCCC GGGCC^C
Asp700I 1697 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1783 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1423 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1010 TGCGCA TGC^GCA
BclI 1037 TGATCA T^GATCA
BglI 2613 GCCTGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 809 AGATCT A^GATCT
BlpI 304 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 304 GCTGAGC GC^T[ACGT]AGC
BsaAI 1803 TACGTG [CT]AC^GT[GA]
BsaBI 1692 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2410 GGCGTC G[GA]^CG[CT]C
BsaI 2464 GGTCTC GGTCTC(1/5)
BsiEI 2630 CGACCG CG[GA][CT]^CG
BsmI 1317 GCATTC GAATGC(1/-1)
BspMI 245 ACCTGC ACCTGC(4/8)
BstAPI 2129 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 304 GCTGAGC GC^T[ACGT]AGC
CpoI 1150 CGGTCCG CG^G[AT]CCG
DraIII 2331 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1783 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1199 AGCGCT AGC^GCT
EcoRI 252 GAATTC G^AATTC
FbaI 1037 TGATCA T^GATCA
FspI 1010 TGCGCA TGC^GCA
Hin1I 2410 GGCGTC G[GA]^CG[CT]C
HincII 1780 GTTGAC GT[CT]^[GA]AC
HindII 1780 GTTGAC GT[CT]^[GA]AC
MamI 1692 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 2655 CACTGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 416 ACATGC [GA]CATG^[CT]
PflFI 1423 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 1150 CGGTCCG CG^G[AT]CCG
SgrAI 1510 CACCGGCG C[GA]^CCGG[CT]G
Tth111I 1423 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 104 TCTAGA T^CTAGA
XmnI 1697 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 252 GAATTC [GA]^AATT[CT]
1668 GAATTT
ApoI 252 GAATTC [GA]^AATT[CT]
1668 GAATTT
AvaI 62 CTCGGG C^[CT]CG[GA]G
1575 CCCGAG
BciVI 1266 GGATAC GTATCC(6/5)
268 GTATCC
BsoBI 62 CTCGGG C^[CT]CG[GA]G
1575 CCCGAG
Bsp1407I 45 TGTACA T^GTACA
881 TGTACA
BsrBI 859 GAGCGG CCGCTC(-3/-3)
2597 GAGCGG
BsrDI 1830 CATTGC GCAATG(2/0)
1765 GCAATG
BsrFI 1511 ACCGGC [GA]^CCGG[CT]
2057 ACCGGC
BsrGI 45 TGTACA T^GTACA
881 TGTACA
BssSI 26 CTCGTG CACGAG(-5/-1)
976 CTCGTG
BstXI 1480 CCACTAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1597 CCAGCCCTGTGG
Cfr10I 1511 ACCGGC [GA]^CCGG[CT]
2057 ACCGGC
DrdI 1954 GACCTCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2626 GACTCGACCGTC
EcoNI 1571 CCTACCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2103 CCTTGGGCAGG
HaeII 963 GGCGCT [GA]GCGC^[CT]
1199 AGCGCT
MseI 94 TTAA T^TAA
2698 TTAA
PflMI 1436 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1993 CCATGCCATGG
PstI 1519 CTGCAG CTGCA^G
1624 CTGCAG
SacI 307 GAGCTC GAGCT^C
973 GAGCTC
SfcI 1519 CTGCAG C^T[GA][CT]AG
1624 CTGCAG
SspBI 45 TGTACA T^GTACA
881 TGTACA
Tru9I 94 TTAA T^TAA
2698 TTAA
Van91I 1436 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1993 CCATGCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI StuI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII