##################################
# NO CUT ENZYMES (   92 enzymes) #
##################################

AatII       AclI        AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        AspEI       AviII       AvrII       
BbeI        BbrPI       BbvCI       BciVI       BclI        
BfrI        BglI        BlnI        BsaAI       BsaBI       
BsiEI       BsiWI       BspDI       BsrBI       BsrFI       
BstAPI      BstBI       Bsu36I      Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoT22I     FbaI        FseI        FspI        HaeII       
HincII      HindII      HindIII     HpaI        KasI        
MamI        MfeI        MluI        MunI        NaeI        
NarI        NgoMIV      NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SalI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        SspI        StuI        SwaI        
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

Acc65I       2534  GGTACC               G^GTACC
AcyI         1038  GACGCC               G[GA]^CG[CT]C
AflIII       2653  ACATGT               A^C[GA][CT]GT
Asp700I       512  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2534  GGTACC               G^GTACC
BalI         1390  TGGCCA               TGG^CCA
BamHI          46  GGATCC               G^GATCC
BcgI          312  CGAGAAATCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI        1038  GACGCC               G[GA]^CG[CT]C
BsmBI        1094  CGTCTC               CGTCTC(1/5)
BsmI         2366  GAATGC               GAATGC(1/-1)
BspHI          94  TCATGA               T^CATGA
BspLU11I     2653  ACATGT               A^CATGT
BsrDI        1891  CATTGC               GCAATG(2/0)
BssHII        188  GCGCGC               G^CGCGC
BssSI         310  CACGAG               CACGAG(-5/-1)
Bst1107I      797  GTATAC               GTA^TAC
BstEII       1355  GGTCACC              G^GT[ACGT]ACC
BstPI        1355  GGTCACC              G^GT[ACGT]ACC
BstZ17I       797  GTATAC               GTA^TAC
EcoO65I      1355  GGTCACC              G^GT[ACGT]ACC
EcoRI        1491  GAATTC               G^AATTC
EcoRV        1203  GATATC               GAT^ATC
Hin1I        1038  GACGCC               G[GA]^CG[CT]C
KpnI         2534  GGTACC               GGTAC^C
KspI         2414  CCGCGG               CCGC^GG
MluNI        1390  TGGCCA               TGG^CCA
MscI         1390  TGGCCA               TGG^CCA
MseI          946  TTAA                 T^TAA
NdeI         2932  CATATG               CA^TATG
NheI          762  GCTAGC               G^CTAGC
NspI         2653  ACATGT               [GA]CATG^[CT]
RcaI           94  TCATGA               T^CATGA
SacII        2414  CCGCGG               CCGC^GG
TfiI         3143  GAATC                G^A[AT]TC
Tru9I         946  TTAA                 T^TAA
XmnI          512  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccI          797  GTATAC               GT^[AC][GT]AC
             2061  GTCTAC              
AccII         189  CGCG                 CG^CG
             2415  CGCG                
AccIII        508  TCCGGA               T^CCGGA
             1517  TCCGGA              
AcsI          110  GAATTT               [GA]^AATT[CT]
             1491  GAATTC              
ApaI           34  GGGCCC               GGGCC^C
              141  GGGCCC              
ApoI          110  GAATTT               [GA]^AATT[CT]
             1491  GAATTC              
AspI          740  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2196  GACAAGGTC           
BbsI         1886  GAAGAC               GAAGAC(2/6)
              856  GTCTTC              
BpuAI        1886  GAAGAC               GAAGAC(2/6)
              856  GTCTTC              
BseAI         508  TCCGGA               T^CCGGA
             1517  TCCGGA              
Bsp1407I     2656  TGTACA               T^GTACA
             2936  TGTACA              
BspEI         508  TCCGGA               T^CCGGA
             1517  TCCGGA              
BspMI        2920  ACCTGC               ACCTGC(4/8)
             2246  GCAGGT              
BsrGI        2656  TGTACA               T^GTACA
             2936  TGTACA              
BstUI         189  CGCG                 CG^CG
             2415  CGCG                
EaeI         1390  TGGCCA               [CT]^GGCC[GA]
             2992  CGGCCA              
HgaI          926  GACGC                GACGC(5/10)
             1038  GACGC               
MroI          508  TCCGGA               T^CCGGA
             1517  TCCGGA              
MvnI          189  CGCG                 CG^CG
             2415  CGCG                
PflFI         740  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2196  GACAAGGTC           
PflMI         422  CCATTTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              943  CCATTAACTGG         
SacI          564  GAGCTC               GAGCT^C
             1725  GAGCTC              
SapI         1833  GAAGAGC              GCTCTTC(1/4)
              872  GCTCTTC             
SmaI           37  CCCGGG               CCC^GGG
             2434  CCCGGG              
SmlI         1677  CTCAAG               C^T[CT][GA]AG
             2240  CTTGAG              
SspBI        2656  TGTACA               T^GTACA
             2936  TGTACA              
TaqI          807  TCGA                 T^CGA
             1948  TCGA                
Tth111I       740  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2196  GACAAGGTC           
TthHB8I       807  TCGA                 T^CGA
             1948  TCGA                
Van91I        422  CCATTTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              943  CCATTAACTGG         
XmaCI          37  CCCGGG               C^CCGGG
             2434  CCCGGG              
XmaI           37  CCCGGG               C^CCGGG
             2434  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       AvaI        AvaII       BanI        BanII       
BbvI        BcnI        BfaI        BglII       BlpI        
BmyI        BpmI        Bpu1102I    BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstXI       
BstYI       Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
SbfI        ScrFI       SfaNI       SfcI        Sse8387I    
StyI        TseI        Tsp45I      Tsp509I     TspRI       
XcmI        XhoII