##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI AspEI AviII AvrII
BbeI BbrPI BbvCI BciVI BclI
BfrI BglI BlnI BsaAI BsaBI
BsiEI BsiWI BspDI BsrBI BsrFI
BstAPI BstBI Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoT22I FbaI FseI FspI HaeII
HincII HindII HindIII HpaI KasI
MamI MfeI MluI MunI NaeI
NarI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SalI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI SspI StuI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 2534 GGTACC G^GTACC
AcyI 1038 GACGCC G[GA]^CG[CT]C
AflIII 2653 ACATGT A^C[GA][CT]GT
Asp700I 512 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2534 GGTACC G^GTACC
BalI 1390 TGGCCA TGG^CCA
BamHI 46 GGATCC G^GATCC
BcgI 312 CGAGAAATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 1038 GACGCC G[GA]^CG[CT]C
BsmBI 1094 CGTCTC CGTCTC(1/5)
BsmI 2366 GAATGC GAATGC(1/-1)
BspHI 94 TCATGA T^CATGA
BspLU11I 2653 ACATGT A^CATGT
BsrDI 1891 CATTGC GCAATG(2/0)
BssHII 188 GCGCGC G^CGCGC
BssSI 310 CACGAG CACGAG(-5/-1)
Bst1107I 797 GTATAC GTA^TAC
BstEII 1355 GGTCACC G^GT[ACGT]ACC
BstPI 1355 GGTCACC G^GT[ACGT]ACC
BstZ17I 797 GTATAC GTA^TAC
EcoO65I 1355 GGTCACC G^GT[ACGT]ACC
EcoRI 1491 GAATTC G^AATTC
EcoRV 1203 GATATC GAT^ATC
Hin1I 1038 GACGCC G[GA]^CG[CT]C
KpnI 2534 GGTACC GGTAC^C
KspI 2414 CCGCGG CCGC^GG
MluNI 1390 TGGCCA TGG^CCA
MscI 1390 TGGCCA TGG^CCA
MseI 946 TTAA T^TAA
NdeI 2932 CATATG CA^TATG
NheI 762 GCTAGC G^CTAGC
NspI 2653 ACATGT [GA]CATG^[CT]
RcaI 94 TCATGA T^CATGA
SacII 2414 CCGCGG CCGC^GG
TfiI 3143 GAATC G^A[AT]TC
Tru9I 946 TTAA T^TAA
XmnI 512 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 797 GTATAC GT^[AC][GT]AC
2061 GTCTAC
AccII 189 CGCG CG^CG
2415 CGCG
AccIII 508 TCCGGA T^CCGGA
1517 TCCGGA
AcsI 110 GAATTT [GA]^AATT[CT]
1491 GAATTC
ApaI 34 GGGCCC GGGCC^C
141 GGGCCC
ApoI 110 GAATTT [GA]^AATT[CT]
1491 GAATTC
AspI 740 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2196 GACAAGGTC
BbsI 1886 GAAGAC GAAGAC(2/6)
856 GTCTTC
BpuAI 1886 GAAGAC GAAGAC(2/6)
856 GTCTTC
BseAI 508 TCCGGA T^CCGGA
1517 TCCGGA
Bsp1407I 2656 TGTACA T^GTACA
2936 TGTACA
BspEI 508 TCCGGA T^CCGGA
1517 TCCGGA
BspMI 2920 ACCTGC ACCTGC(4/8)
2246 GCAGGT
BsrGI 2656 TGTACA T^GTACA
2936 TGTACA
BstUI 189 CGCG CG^CG
2415 CGCG
EaeI 1390 TGGCCA [CT]^GGCC[GA]
2992 CGGCCA
HgaI 926 GACGC GACGC(5/10)
1038 GACGC
MroI 508 TCCGGA T^CCGGA
1517 TCCGGA
MvnI 189 CGCG CG^CG
2415 CGCG
PflFI 740 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2196 GACAAGGTC
PflMI 422 CCATTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
943 CCATTAACTGG
SacI 564 GAGCTC GAGCT^C
1725 GAGCTC
SapI 1833 GAAGAGC GCTCTTC(1/4)
872 GCTCTTC
SmaI 37 CCCGGG CCC^GGG
2434 CCCGGG
SmlI 1677 CTCAAG C^T[CT][GA]AG
2240 CTTGAG
SspBI 2656 TGTACA T^GTACA
2936 TGTACA
TaqI 807 TCGA T^CGA
1948 TCGA
Tth111I 740 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2196 GACAAGGTC
TthHB8I 807 TCGA T^CGA
1948 TCGA
Van91I 422 CCATTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
943 CCATTAACTGG
XmaCI 37 CCCGGG C^CCGGG
2434 CCCGGG
XmaI 37 CCCGGG C^CCGGG
2434 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BfaI BglII BlpI
BmyI BpmI Bpu1102I BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI Sse8387I
StyI TseI Tsp45I Tsp509I TspRI
XcmI XhoII