################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI AspEI AviII AvrII BbeI BbrPI BbvCI BciVI BclI BfrI BglI BlnI BsaAI BsaBI BsiEI BsiWI BspDI BsrBI BsrFI BstAPI BstBI Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI MamI MfeI MluI MunI NaeI NarI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspI StuI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### Acc65I 2534 GGTACC G^GTACC AcyI 1038 GACGCC G[GA]^CG[CT]C AflIII 2653 ACATGT A^C[GA][CT]GT Asp700I 512 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2534 GGTACC G^GTACC BalI 1390 TGGCCA TGG^CCA BamHI 46 GGATCC G^GATCC BcgI 312 CGAGAAATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaHI 1038 GACGCC G[GA]^CG[CT]C BsmBI 1094 CGTCTC CGTCTC(1/5) BsmI 2366 GAATGC GAATGC(1/-1) BspHI 94 TCATGA T^CATGA BspLU11I 2653 ACATGT A^CATGT BsrDI 1891 CATTGC GCAATG(2/0) BssHII 188 GCGCGC G^CGCGC BssSI 310 CACGAG CACGAG(-5/-1) Bst1107I 797 GTATAC GTA^TAC BstEII 1355 GGTCACC G^GT[ACGT]ACC BstPI 1355 GGTCACC G^GT[ACGT]ACC BstZ17I 797 GTATAC GTA^TAC EcoO65I 1355 GGTCACC G^GT[ACGT]ACC EcoRI 1491 GAATTC G^AATTC EcoRV 1203 GATATC GAT^ATC Hin1I 1038 GACGCC G[GA]^CG[CT]C KpnI 2534 GGTACC GGTAC^C KspI 2414 CCGCGG CCGC^GG MluNI 1390 TGGCCA TGG^CCA MscI 1390 TGGCCA TGG^CCA MseI 946 TTAA T^TAA NdeI 2932 CATATG CA^TATG NheI 762 GCTAGC G^CTAGC NspI 2653 ACATGT [GA]CATG^[CT] RcaI 94 TCATGA T^CATGA SacII 2414 CCGCGG CCGC^GG TfiI 3143 GAATC G^A[AT]TC Tru9I 946 TTAA T^TAA XmnI 512 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 797 GTATAC GT^[AC][GT]AC 2061 GTCTAC AccII 189 CGCG CG^CG 2415 CGCG AccIII 508 TCCGGA T^CCGGA 1517 TCCGGA AcsI 110 GAATTT [GA]^AATT[CT] 1491 GAATTC ApaI 34 GGGCCC GGGCC^C 141 GGGCCC ApoI 110 GAATTT [GA]^AATT[CT] 1491 GAATTC AspI 740 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2196 GACAAGGTC BbsI 1886 GAAGAC GAAGAC(2/6) 856 GTCTTC BpuAI 1886 GAAGAC GAAGAC(2/6) 856 GTCTTC BseAI 508 TCCGGA T^CCGGA 1517 TCCGGA Bsp1407I 2656 TGTACA T^GTACA 2936 TGTACA BspEI 508 TCCGGA T^CCGGA 1517 TCCGGA BspMI 2920 ACCTGC ACCTGC(4/8) 2246 GCAGGT BsrGI 2656 TGTACA T^GTACA 2936 TGTACA BstUI 189 CGCG CG^CG 2415 CGCG EaeI 1390 TGGCCA [CT]^GGCC[GA] 2992 CGGCCA HgaI 926 GACGC GACGC(5/10) 1038 GACGC MroI 508 TCCGGA T^CCGGA 1517 TCCGGA MvnI 189 CGCG CG^CG 2415 CGCG PflFI 740 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2196 GACAAGGTC PflMI 422 CCATTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 943 CCATTAACTGG SacI 564 GAGCTC GAGCT^C 1725 GAGCTC SapI 1833 GAAGAGC GCTCTTC(1/4) 872 GCTCTTC SmaI 37 CCCGGG CCC^GGG 2434 CCCGGG SmlI 1677 CTCAAG C^T[CT][GA]AG 2240 CTTGAG SspBI 2656 TGTACA T^GTACA 2936 TGTACA TaqI 807 TCGA T^CGA 1948 TCGA Tth111I 740 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2196 GACAAGGTC TthHB8I 807 TCGA T^CGA 1948 TCGA Van91I 422 CCATTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 943 CCATTAACTGG XmaCI 37 CCCGGG C^CCGGG 2434 CCCGGG XmaI 37 CCCGGG C^CCGGG 2434 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI Sse8387I StyI TseI Tsp45I Tsp509I TspRI XcmI XhoII