################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## Acc65I AccIII AclI AflII AflIII AscI AseI AsnI Asp718I AviII AvrII BamHI BbeI BbrPI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspLU11I Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII EagI EclXI Eco52I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SbfI ScaI SexAI SfiI SfoI SfuI SnaBI SpeI SphI Sse8387I SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 1499 GACGTC GACGT^C AccI 170 GTCGAC GT^[AC][GT]AC AgeI 476 ACCGGT A^CCGGT AhdI 1137 GACATCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 133 AGCGCT AGC^GCT Asp700I 1923 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1137 GACATCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2286 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 246 CCCGGG C^[CT]CG[GA]G BsaAI 1181 TACGTG [CT]AC^GT[GA] BsaWI 476 ACCGGT [AT]^CCGG[AT] BsoBI 246 CCCGGG C^[CT]CG[GA]G Bsp1407I 2249 TGTACA T^GTACA BspHI 382 TCATGA T^CATGA BsrBI 31 CCGCTC CCGCTC(-3/-3) BsrGI 2249 TGTACA T^GTACA BstXI 1933 CCAGCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 973 GGATCT [GA]^GATC[CT] Eam1105I 1137 GACATCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 133 AGCGCT AGC^GCT EcoRI 2128 GAATTC G^AATTC EcoT14I 2002 CCTTGG C^C[AT][AT]GG HaeII 133 AGCGCT [GA]GCGC^[CT] MflI 973 GGATCT [GA]^GATC[CT] MseI 510 TTAA T^TAA PflFI 2286 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 709 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 476 ACCGGT A^CCGGT RcaI 382 TCATGA T^CATGA SacI 297 GAGCTC GAGCT^C SalI 170 GTCGAC G^TCGAC SapI 1381 GCTCTTC GCTCTTC(1/4) SgrAI 475 CACCGGTG C[GA]^CCGG[CT]G SmaI 246 CCCGGG CCC^GGG SspBI 2249 TGTACA T^GTACA StyI 2002 CCTTGG C^C[AT][AT]GG TaqI 171 TCGA T^CGA TfiI 908 GAATC G^A[AT]TC Tru9I 510 TTAA T^TAA Tth111I 2286 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 171 TCGA T^CGA Van91I 709 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2615 TCTAGA T^CTAGA XhoII 973 GGATCT [GA]^GATC[CT] XmaCI 246 CCCGGG C^CCGGG XmaI 246 CCCGGG C^CCGGG XmnI 1923 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcyI 937 GGCGTC G[GA]^CG[CT]C 1499 GACGTC Alw44I 434 GTGCAC G^TGCAC 480 GTGCAC AlwI 950 GATCC GGATC(4/5) 973 GGATC ApaLI 434 GTGCAC G^TGCAC 480 GTGCAC BanI 41 GGCACC G^G[CT][GA]CC 1985 GGCACC BbvCI 842 CCTCAGC CCTCAGC(-5/-2) 1866 CCTCAGC BcgI 10 CGACCCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 602 GCAGCAGCATCG BsaHI 937 GGCGTC G[GA]^CG[CT]C 1499 GACGTC BsmI 69 GCATTC GAATGC(1/-1) 1861 GCATTC BspMI 166 GCAGGT ACCTGC(4/8) 2194 GCAGGT BssSI 779 CACGAG CACGAG(-5/-1) 368 CTCGTG Hin1I 937 GGCGTC G[GA]^CG[CT]C 1499 GACGTC MslI 364 CACCCTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2153 CACTGGAATG NaeI 1307 GCCGGC GCC^GGC 2044 GCCGGC NgoMIV 1307 GCCGGC G^CCGGC 2044 GCCGGC NspI 1544 GCATGT [GA]CATG^[CT] 2489 ACATGC PstI 525 CTGCAG CTGCA^G 864 CTGCAG PvuII 831 CAGCTG CAG^CTG 2513 CAGCTG SmlI 1911 CTCAAG C^T[CT][GA]AG 2096 CTTGAG StuI 186 AGGCCT AGG^CCT 851 AGGCCT XcmI 873 CCACGACCAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1242 CCAACTTCCCTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwNI AocI ApaI ApoI AspHI AvaII BalI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI Eco81I EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TseI Tsp45I Tsp509I TspRI