##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AscI AseI AsnI Asp718I AviII
AvrII BamHI BbeI BbrPI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DraIII
EagI EclXI Eco52I EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SbfI
ScaI SexAI SfiI SfoI SfuI
SnaBI SpeI SphI Sse8387I SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1499 GACGTC GACGT^C
AccI 170 GTCGAC GT^[AC][GT]AC
AgeI 476 ACCGGT A^CCGGT
AhdI 1137 GACATCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 133 AGCGCT AGC^GCT
Asp700I 1923 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1137 GACATCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2286 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 246 CCCGGG C^[CT]CG[GA]G
BsaAI 1181 TACGTG [CT]AC^GT[GA]
BsaWI 476 ACCGGT [AT]^CCGG[AT]
BsoBI 246 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2249 TGTACA T^GTACA
BspHI 382 TCATGA T^CATGA
BsrBI 31 CCGCTC CCGCTC(-3/-3)
BsrGI 2249 TGTACA T^GTACA
BstXI 1933 CCAGCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 973 GGATCT [GA]^GATC[CT]
Eam1105I 1137 GACATCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 133 AGCGCT AGC^GCT
EcoRI 2128 GAATTC G^AATTC
EcoT14I 2002 CCTTGG C^C[AT][AT]GG
HaeII 133 AGCGCT [GA]GCGC^[CT]
MflI 973 GGATCT [GA]^GATC[CT]
MseI 510 TTAA T^TAA
PflFI 2286 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 709 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 476 ACCGGT A^CCGGT
RcaI 382 TCATGA T^CATGA
SacI 297 GAGCTC GAGCT^C
SalI 170 GTCGAC G^TCGAC
SapI 1381 GCTCTTC GCTCTTC(1/4)
SgrAI 475 CACCGGTG C[GA]^CCGG[CT]G
SmaI 246 CCCGGG CCC^GGG
SspBI 2249 TGTACA T^GTACA
StyI 2002 CCTTGG C^C[AT][AT]GG
TaqI 171 TCGA T^CGA
TfiI 908 GAATC G^A[AT]TC
Tru9I 510 TTAA T^TAA
Tth111I 2286 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 171 TCGA T^CGA
Van91I 709 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2615 TCTAGA T^CTAGA
XhoII 973 GGATCT [GA]^GATC[CT]
XmaCI 246 CCCGGG C^CCGGG
XmaI 246 CCCGGG C^CCGGG
XmnI 1923 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 937 GGCGTC G[GA]^CG[CT]C
1499 GACGTC
Alw44I 434 GTGCAC G^TGCAC
480 GTGCAC
AlwI 950 GATCC GGATC(4/5)
973 GGATC
ApaLI 434 GTGCAC G^TGCAC
480 GTGCAC
BanI 41 GGCACC G^G[CT][GA]CC
1985 GGCACC
BbvCI 842 CCTCAGC CCTCAGC(-5/-2)
1866 CCTCAGC
BcgI 10 CGACCCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
602 GCAGCAGCATCG
BsaHI 937 GGCGTC G[GA]^CG[CT]C
1499 GACGTC
BsmI 69 GCATTC GAATGC(1/-1)
1861 GCATTC
BspMI 166 GCAGGT ACCTGC(4/8)
2194 GCAGGT
BssSI 779 CACGAG CACGAG(-5/-1)
368 CTCGTG
Hin1I 937 GGCGTC G[GA]^CG[CT]C
1499 GACGTC
MslI 364 CACCCTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2153 CACTGGAATG
NaeI 1307 GCCGGC GCC^GGC
2044 GCCGGC
NgoMIV 1307 GCCGGC G^CCGGC
2044 GCCGGC
NspI 1544 GCATGT [GA]CATG^[CT]
2489 ACATGC
PstI 525 CTGCAG CTGCA^G
864 CTGCAG
PvuII 831 CAGCTG CAG^CTG
2513 CAGCTG
SmlI 1911 CTCAAG C^T[CT][GA]AG
2096 CTTGAG
StuI 186 AGGCCT AGG^CCT
851 AGGCCT
XcmI 873 CCACGACCAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1242 CCAACTTCCCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwNI AocI ApaI ApoI AspHI
AvaII BalI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI Eco81I EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
Tsp45I Tsp509I TspRI