##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AccIII AclI AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI AviII
AvrII BbeI BbrPI BbvCI BcgI
BciVI BclI BlnI BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspLU11I BsrFI
BssHII BssSI BstBI BstXI CfoI
Cfr10I ClaI CpoI DraI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI HhaI HinP1I HincII
HindII HpaI KasI KspI MamI
MluI MroI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 1231 GACGTC GACGT^C
Acc65I 1425 GGTACC G^GTACC
AccI 2104 GTATAC GT^[AC][GT]AC
AccII 1337 CGCG CG^CG
AcyI 1231 GACGTC G[GA]^CG[CT]C
AflII 2046 CTTAAG C^TTAAG
AocI 583 CCTGAGG CC^T[ACGT]AGG
Asp718I 1425 GGTACC G^GTACC
AspI 2003 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1992 CTCGGG C^[CT]CG[GA]G
BamHI 588 GGATCC G^GATCC
BfrI 2046 CTTAAG C^TTAAG
BglI 811 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 272 AGATCT A^GATCT
BlpI 1557 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1557 GCTGAGC GC^T[ACGT]AGC
BsaHI 1231 GACGTC G[GA]^CG[CT]C
BsaI 1056 GGTCTC GGTCTC(1/5)
BsoBI 1992 CTCGGG C^[CT]CG[GA]G
BspMI 1256 GCAGGT ACCTGC(4/8)
Bst1107I 2104 GTATAC GTA^TAC
BstAPI 2135 GCACTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 315 GGTGACC G^GT[ACGT]ACC
BstPI 315 GGTGACC G^GT[ACGT]ACC
BstUI 1337 CGCG CG^CG
BstZ17I 2104 GTATAC GTA^TAC
Bsu36I 583 CCTGAGG CC^T[ACGT]AGG
CelII 1557 GCTGAGC GC^T[ACGT]AGC
DrdI 2069 GACATCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1398 GAAGAG CTCTTC(1/4)
Eco81I 583 CCTGAGG CC^T[ACGT]AGG
EcoNI 1684 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 315 GGTGACC G^GT[ACGT]ACC
EcoRI 701 GAATTC G^AATTC
Hin1I 1231 GACGTC G[GA]^CG[CT]C
HindIII 1453 AAGCTT A^AGCTT
KpnI 1425 GGTACC GGTAC^C
Ksp632I 1398 GAAGAG CTCTTC(1/4)
MaeII 1232 ACGT A^CGT
MfeI 1392 CAATTG C^AATTG
MunI 1392 CAATTG C^AATTG
MvnI 1337 CGCG CG^CG
PflFI 2003 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1678 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1254 CTGCAG CTGCA^G
SacI 1598 GAGCTC GAGCT^C
SapI 1398 GAAGAGC GCTCTTC(1/4)
ScaI 1967 AGTACT AGT^ACT
SexAI 35 ACCTGGT A^CC[AT]GGT
Tth111I 2003 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1678 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 701 GAATTC [GA]^AATT[CT]
1379 AAATTT
ApaI 660 GGGCCC GGGCC^C
1162 GGGCCC
ApoI 701 GAATTC [GA]^AATT[CT]
1379 AAATTT
BalI 304 TGGCCA TGG^CCA
969 TGGCCA
BanI 1425 GGTACC G^G[CT][GA]CC
2216 GGTGCC
BpmI 46 CTGGAG CTGGAG(16/14)
419 CTGGAG
BsgI 40 GTGCAG GTGCAG(16/14)
289 GTGCAG
BspHI 552 TCATGA T^CATGA
2151 TCATGA
BsrBI 2336 CCGCTC CCGCTC(-3/-3)
151 GAGCGG
BsrDI 633 CATTGC GCAATG(2/0)
1512 CATTGC
DraIII 34 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
2136 CACTGGGTG
FauI 1335 CCCGC CCCGC(4/6)
351 GCGGG
MluNI 304 TGGCCA TGG^CCA
969 TGGCCA
MscI 304 TGGCCA TGG^CCA
969 TGGCCA
PshAI 1050 GACAGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2155 GACAGCTGTC
PvuII 43 CAGCTG CAG^CTG
2157 CAGCTG
RcaI 552 TCATGA T^CATGA
2151 TCATGA
Tsp45I 316 GTGAC ^GT[GC]AC
1117 GTCAC
XcmI 677 CCATCAGTTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2301 CCACCCTCTGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbsI BbvI BcnI
BfaI BmyI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII