##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AclI AcyI AflIII
AgeI Aor51HI AscI AseI AsnI
Asp700I Asp718I AviII AvrII BamHI
BbeI BbrPI BciVI BglII BlnI
BsaBI BsaHI BsaI BsgI BsiEI
BsiWI BspDI BspLU11I BsrBI BsrFI
BssHII Bst1107I BstBI BstXI BstZ17I
Cfr10I ClaI CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoRV
FseI FspI HaeII Hin1I HpaI
KasI KpnI KspI MamI MluI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SwaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccII 17 CGCG CG^CG
AccIII 626 TCCGGA T^CCGGA
AflII 2948 CTTAAG C^TTAAG
AhdI 23 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2490 GTGCAC G^TGCAC
ApaI 97 GGGCCC GGGCC^C
ApaLI 2490 GTGCAC G^TGCAC
AspEI 23 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2490 GTGCAC G[AT]GC[AT]^C
AspI 2824 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1618 CCCGAG C^[CT]CG[GA]G
BalI 222 TGGCCA TGG^CCA
BanI 532 GGCACC G^G[CT][GA]CC
BcgI 2416 CGAGTGCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 486 TGATCA T^GATCA
BfrI 2948 CTTAAG C^TTAAG
BglI 1867 GCCTGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2629 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2629 GCTTAGC GC^T[ACGT]AGC
BsaAI 1102 TACGTG [CT]AC^GT[GA]
BsaWI 626 TCCGGA [AT]^CCGG[AT]
BseAI 626 TCCGGA T^CCGGA
BsiHKAI 2490 GTGCAC G[AT]GC[AT]^C
BsmBI 21 GAGACG CGTCTC(1/5)
BsmI 937 GAATGC GAATGC(1/-1)
BsoBI 1618 CCCGAG C^[CT]CG[GA]G
BspEI 626 TCCGGA T^CCGGA
BspHI 603 TCATGA T^CATGA
BsrDI 1826 GCAATG GCAATG(2/0)
BssSI 1954 CACGAG CACGAG(-5/-1)
BstUI 17 CGCG CG^CG
CelII 2629 GCTTAGC GC^T[ACGT]AGC
CfoI 2115 GCGC GCG^C
Eam1105I 23 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2559 CCTTTAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1083 GAATTC G^AATTC
FbaI 486 TGATCA T^GATCA
HhaI 2115 GCGC GCG^C
HinP1I 2115 GCGC G^CGC
HincII 474 GTTGAC GT[CT]^[GA]AC
HindII 474 GTTGAC GT[CT]^[GA]AC
HindIII 144 AAGCTT A^AGCTT
MaeII 1103 ACGT A^CGT
MfeI 422 CAATTG C^AATTG
MluNI 222 TGGCCA TGG^CCA
MroI 626 TCCGGA T^CCGGA
MscI 222 TGGCCA TGG^CCA
MunI 422 CAATTG C^AATTG
MvnI 17 CGCG CG^CG
NcoI 321 CCATGG C^CATGG
PflFI 2824 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2952 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 603 TCATGA T^CATGA
ScaI 1817 AGTACT AGT^ACT
SfiI 1866 GGCCTGGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 2700 ACTAGT A^CTAGT
SspI 1144 AATATT AAT^ATT
StuI 2614 AGGCCT AGG^CCT
Tth111I 2824 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1879 TCTAGA T^CTAGA
XcmI 1113 CCAAGCCTCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 2046 GTAGAC GT^[AC][GT]AC
2789 GTAGAC
AlwNI 731 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1337 CAGCTGCTG
AocI 559 CCTGAGG CC^T[ACGT]AGG
1684 CCTGAGG
BbvCI 1402 CCTCAGC CCTCAGC(-5/-2)
1750 CCTCAGC
BseRI 249 CTCCTC GAGGAG(10/8)
1382 CTCCTC
Bsp1407I 866 TGTACA T^GTACA
3056 TGTACA
BsrGI 866 TGTACA T^GTACA
3056 TGTACA
BstEII 1236 GGTTACC G^GT[ACGT]ACC
3082 GGTTACC
BstPI 1236 GGTTACC G^GT[ACGT]ACC
3082 GGTTACC
Bsu36I 559 CCTGAGG CC^T[ACGT]AGG
1684 CCTGAGG
DraII 97 GGGCCCT [GA]G^G[ACGT]CC[CT]
2952 AGGTCCC
DsaI 321 CCATGG C^C[GA][CT]GG
1245 CCGTGG
EaeI 222 TGGCCA [CT]^GGCC[GA]
2129 CGGCCA
Eco81I 559 CCTGAGG CC^T[ACGT]AGG
1684 CCTGAGG
EcoO109I 97 GGGCCCT [GA]G^G[ACGT]CC[CT]
2952 AGGTCCC
EcoO65I 1236 GGTTACC G^GT[ACGT]ACC
3082 GGTTACC
EcoT22I 743 ATGCAT ATGCA^T
2726 ATGCAT
FauI 640 CCCGC CCCGC(4/6)
341 GCGGG
HgaI 15 GACGC GACGC(5/10)
23 GACGC
MspA1I 1331 CAGCTG C[AC]G^C[GT]G
1337 CAGCTG
NsiI 743 ATGCAT ATGCA^T
2726 ATGCAT
NspI 302 ACATGC [GA]CATG^[CT]
3095 GCATGT
PflMI 291 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2092 CCAAGTGCTGG
PvuII 1331 CAGCTG CAG^CTG
1337 CAGCTG
SmlI 904 CTCAAG C^T[CT][GA]AG
2948 CTTAAG
SspBI 866 TGTACA T^GTACA
3056 TGTACA
Van91I 291 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2092 CCAAGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII