################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII AflII AflIII AgeI AhdI ApaI AscI AspEI AviII AvrII BalI BbrPI BbsI BcgI BfrI BglII BlnI BpuAI BsiEI BsiWI BsmBI BspDI BspHI BspLU11I BsrBI BssHII BssSI BstBI ClaI DrdI EagI Eam1105I EclXI Eco52I EcoNI FauI FspI KspI MluI MluNI MscI NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PvuI RcaI SacII SalI SbfI SexAI SfuI SgrAI SphI Sse8387I SwaI Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 68 enzymes) # ################################### Acc65I 427 GGTACC G^GTACC AccI 1295 GTATAC GT^[AC][GT]AC AccII 335 CGCG CG^CG AccIII 7 TCCGGA T^CCGGA AclI 1170 AACGTT AA^CGTT AcyI 79 GGCGCC G[GA]^CG[CT]C Alw44I 2008 GTGCAC G^TGCAC ApaLI 2008 GTGCAC G^TGCAC AseI 3336 ATTAAT AT^TAAT AsnI 3336 ATTAAT AT^TAAT Asp718I 427 GGTACC G^GTACC AspI 248 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 498 GGATCC G^GATCC BbeI 79 GGCGCC GGCGC^C BbvCI 526 GCTGAGG CCTCAGC(-5/-2) BglI 228 GCCGAGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 3526 TACGTA [CT]AC^GT[GA] BsaBI 1187 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 79 GGCGCC G[GA]^CG[CT]C BsaI 740 GAGACC GGTCTC(1/5) BseAI 7 TCCGGA T^CCGGA BspEI 7 TCCGGA T^CCGGA BspMI 50 GCAGGT ACCTGC(4/8) BsrFI 214 GCCGGC [GA]^CCGG[CT] Bst1107I 1295 GTATAC GTA^TAC BstEII 1240 GGTTACC G^GT[ACGT]ACC BstPI 1240 GGTTACC G^GT[ACGT]ACC BstUI 335 CGCG CG^CG BstXI 1960 CCATCTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1295 GTATAC GTA^TAC Cfr10I 214 GCCGGC [GA]^CCGG[CT] CpoI 9 CGGACCG CG^G[AT]CCG EcoO65I 1240 GGTTACC G^GT[ACGT]ACC EcoRV 840 GATATC GAT^ATC FseI 213 GGCCGGCC GGCCGG^CC HgaI 277 GACGC GACGC(5/10) Hin1I 79 GGCGCC G[GA]^CG[CT]C HincII 3392 GTTAAC GT[CT]^[GA]AC HindII 3392 GTTAAC GT[CT]^[GA]AC HpaI 3392 GTTAAC GTT^AAC KasI 79 GGCGCC G^GCGCC KpnI 427 GGTACC GGTAC^C MamI 1187 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3637 CAATTG C^AATTG MroI 7 TCCGGA T^CCGGA MunI 3637 CAATTG C^AATTG MvnI 335 CGCG CG^CG NaeI 214 GCCGGC GCC^GGC NarI 79 GGCGCC GG^CGCC NdeI 958 CATATG CA^TATG NgoMIV 214 GCCGGC G^CCGGC NspI 2058 ACATGC [GA]CATG^[CT] PflFI 248 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 3336 ATTAAT AT^TAAT Psp1406I 1170 AACGTT AA^CGTT PstI 1653 CTGCAG CTGCA^G PvuII 2200 CAGCTG CAG^CTG RsrII 9 CGGACCG CG^G[AT]CCG SfiI 227 GGCCGAGTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 79 GGCGCC GGC^GCC SmaI 176 CCCGGG CCC^GGG SmlI 2647 CTTGAG C^T[CT][GA]AG SnaBI 3526 TACGTA TAC^GTA SpeI 3352 ACTAGT A^CTAGT Tth111I 248 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 412 CCATGGCCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 176 CCCGGG C^CCGGG XmaI 176 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AlwNI 458 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG 3266 CAGATTCTG AocI 389 CCTCAGG CC^T[ACGT]AGG 493 CCTCAGG Aor51HI 523 AGCGCT AGC^GCT 889 AGCGCT Asp700I 1351 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1855 GAAAGCCTTC AvaI 176 CCCGGG C^[CT]CG[GA]G 295 CCCGAG BciVI 3632 GGATAC GTATCC(6/5) 3924 GTATCC BclI 1381 TGATCA T^GATCA 3384 TGATCA BlpI 304 GCTCAGC GC^T[ACGT]AGC 779 GCTAAGC Bpu1102I 304 GCTCAGC GC^T[ACGT]AGC 779 GCTAAGC BseRI 3802 CTCCTC GAGGAG(10/8) 529 GAGGAG BsgI 260 GTGCAG GTGCAG(16/14) 285 GTGCAG BsoBI 176 CCCGGG C^[CT]CG[GA]G 295 CCCGAG Bsp1407I 1885 TGTACA T^GTACA 2641 TGTACA BsrGI 1885 TGTACA T^GTACA 2641 TGTACA BstYI 498 GGATCC [GA]^GATC[CT] 3422 AGATCC Bsu36I 389 CCTCAGG CC^T[ACGT]AGG 493 CCTCAGG CelII 304 GCTCAGC GC^T[ACGT]AGC 779 GCTAAGC DraI 2087 TTTAAA TTT^AAA 3818 TTTAAA DraII 172 AGGTCCC [GA]G^G[ACGT]CC[CT] 370 AGGACCC DraIII 903 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG 3787 CACCATGTG Eco47III 523 AGCGCT AGC^GCT 889 AGCGCT Eco81I 389 CCTCAGG CC^T[ACGT]AGG 493 CCTCAGG EcoO109I 172 AGGTCCC [GA]G^G[ACGT]CC[CT] 370 AGGACCC EcoT22I 2066 ATGCAT ATGCA^T 2738 ATGCAT FbaI 1381 TGATCA T^GATCA 3384 TGATCA MflI 498 GGATCC [GA]^GATC[CT] 3422 AGATCC MspA1I 710 CAGCGG C[AC]G^C[GT]G 2200 CAGCTG NsiI 2066 ATGCAT ATGCA^T 2738 ATGCAT PpuMI 172 AGGTCCC [GA]G^G[AT]CC[CT] 370 AGGACCC SacI 1702 GAGCTC GAGCT^C 2206 GAGCTC SapI 815 GAAGAGC GCTCTTC(1/4) 2778 GAAGAGC SspBI 1885 TGTACA T^GTACA 2641 TGTACA StuI 240 AGGCCT AGG^CCT 3798 AGGCCT TaqI 162 TCGA T^CGA 572 TCGA TthHB8I 162 TCGA T^CGA 572 TCGA XhoII 498 GGATCC [GA]^GATC[CT] 3422 AGATCC XmnI 1351 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1855 GAAAGCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BsaWI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrDI BsrI BssKI BstAPI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoRI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI