##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AflII AflIII AgeI AhdI
ApaI AscI AspEI AviII AvrII
BalI BbrPI BbsI BcgI BfrI
BglII BlnI BpuAI BsiEI BsiWI
BsmBI BspDI BspHI BspLU11I BsrBI
BssHII BssSI BstBI ClaI DrdI
EagI Eam1105I EclXI Eco52I EcoNI
FauI FspI KspI MluI MluNI
MscI NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PvuI RcaI
SacII SalI SbfI SexAI SfuI
SgrAI SphI Sse8387I SwaI Van91I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 68 enzymes) #
###################################
Acc65I 427 GGTACC G^GTACC
AccI 1295 GTATAC GT^[AC][GT]AC
AccII 335 CGCG CG^CG
AccIII 7 TCCGGA T^CCGGA
AclI 1170 AACGTT AA^CGTT
AcyI 79 GGCGCC G[GA]^CG[CT]C
Alw44I 2008 GTGCAC G^TGCAC
ApaLI 2008 GTGCAC G^TGCAC
AseI 3336 ATTAAT AT^TAAT
AsnI 3336 ATTAAT AT^TAAT
Asp718I 427 GGTACC G^GTACC
AspI 248 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 498 GGATCC G^GATCC
BbeI 79 GGCGCC GGCGC^C
BbvCI 526 GCTGAGG CCTCAGC(-5/-2)
BglI 228 GCCGAGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 3526 TACGTA [CT]AC^GT[GA]
BsaBI 1187 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 79 GGCGCC G[GA]^CG[CT]C
BsaI 740 GAGACC GGTCTC(1/5)
BseAI 7 TCCGGA T^CCGGA
BspEI 7 TCCGGA T^CCGGA
BspMI 50 GCAGGT ACCTGC(4/8)
BsrFI 214 GCCGGC [GA]^CCGG[CT]
Bst1107I 1295 GTATAC GTA^TAC
BstEII 1240 GGTTACC G^GT[ACGT]ACC
BstPI 1240 GGTTACC G^GT[ACGT]ACC
BstUI 335 CGCG CG^CG
BstXI 1960 CCATCTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1295 GTATAC GTA^TAC
Cfr10I 214 GCCGGC [GA]^CCGG[CT]
CpoI 9 CGGACCG CG^G[AT]CCG
EcoO65I 1240 GGTTACC G^GT[ACGT]ACC
EcoRV 840 GATATC GAT^ATC
FseI 213 GGCCGGCC GGCCGG^CC
HgaI 277 GACGC GACGC(5/10)
Hin1I 79 GGCGCC G[GA]^CG[CT]C
HincII 3392 GTTAAC GT[CT]^[GA]AC
HindII 3392 GTTAAC GT[CT]^[GA]AC
HpaI 3392 GTTAAC GTT^AAC
KasI 79 GGCGCC G^GCGCC
KpnI 427 GGTACC GGTAC^C
MamI 1187 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3637 CAATTG C^AATTG
MroI 7 TCCGGA T^CCGGA
MunI 3637 CAATTG C^AATTG
MvnI 335 CGCG CG^CG
NaeI 214 GCCGGC GCC^GGC
NarI 79 GGCGCC GG^CGCC
NdeI 958 CATATG CA^TATG
NgoMIV 214 GCCGGC G^CCGGC
NspI 2058 ACATGC [GA]CATG^[CT]
PflFI 248 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3336 ATTAAT AT^TAAT
Psp1406I 1170 AACGTT AA^CGTT
PstI 1653 CTGCAG CTGCA^G
PvuII 2200 CAGCTG CAG^CTG
RsrII 9 CGGACCG CG^G[AT]CCG
SfiI 227 GGCCGAGTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 79 GGCGCC GGC^GCC
SmaI 176 CCCGGG CCC^GGG
SmlI 2647 CTTGAG C^T[CT][GA]AG
SnaBI 3526 TACGTA TAC^GTA
SpeI 3352 ACTAGT A^CTAGT
Tth111I 248 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 412 CCATGGCCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 176 CCCGGG C^CCGGG
XmaI 176 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AlwNI 458 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
3266 CAGATTCTG
AocI 389 CCTCAGG CC^T[ACGT]AGG
493 CCTCAGG
Aor51HI 523 AGCGCT AGC^GCT
889 AGCGCT
Asp700I 1351 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1855 GAAAGCCTTC
AvaI 176 CCCGGG C^[CT]CG[GA]G
295 CCCGAG
BciVI 3632 GGATAC GTATCC(6/5)
3924 GTATCC
BclI 1381 TGATCA T^GATCA
3384 TGATCA
BlpI 304 GCTCAGC GC^T[ACGT]AGC
779 GCTAAGC
Bpu1102I 304 GCTCAGC GC^T[ACGT]AGC
779 GCTAAGC
BseRI 3802 CTCCTC GAGGAG(10/8)
529 GAGGAG
BsgI 260 GTGCAG GTGCAG(16/14)
285 GTGCAG
BsoBI 176 CCCGGG C^[CT]CG[GA]G
295 CCCGAG
Bsp1407I 1885 TGTACA T^GTACA
2641 TGTACA
BsrGI 1885 TGTACA T^GTACA
2641 TGTACA
BstYI 498 GGATCC [GA]^GATC[CT]
3422 AGATCC
Bsu36I 389 CCTCAGG CC^T[ACGT]AGG
493 CCTCAGG
CelII 304 GCTCAGC GC^T[ACGT]AGC
779 GCTAAGC
DraI 2087 TTTAAA TTT^AAA
3818 TTTAAA
DraII 172 AGGTCCC [GA]G^G[ACGT]CC[CT]
370 AGGACCC
DraIII 903 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
3787 CACCATGTG
Eco47III 523 AGCGCT AGC^GCT
889 AGCGCT
Eco81I 389 CCTCAGG CC^T[ACGT]AGG
493 CCTCAGG
EcoO109I 172 AGGTCCC [GA]G^G[ACGT]CC[CT]
370 AGGACCC
EcoT22I 2066 ATGCAT ATGCA^T
2738 ATGCAT
FbaI 1381 TGATCA T^GATCA
3384 TGATCA
MflI 498 GGATCC [GA]^GATC[CT]
3422 AGATCC
MspA1I 710 CAGCGG C[AC]G^C[GT]G
2200 CAGCTG
NsiI 2066 ATGCAT ATGCA^T
2738 ATGCAT
PpuMI 172 AGGTCCC [GA]G^G[AT]CC[CT]
370 AGGACCC
SacI 1702 GAGCTC GAGCT^C
2206 GAGCTC
SapI 815 GAAGAGC GCTCTTC(1/4)
2778 GAAGAGC
SspBI 1885 TGTACA T^GTACA
2641 TGTACA
StuI 240 AGGCCT AGG^CCT
3798 AGGCCT
TaqI 162 TCGA T^CGA
572 TCGA
TthHB8I 162 TCGA T^CGA
572 TCGA
XhoII 498 GGATCC [GA]^GATC[CT]
3422 AGATCC
XmnI 1351 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1855 GAAAGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BsaWI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrDI
BsrI BssKI BstAPI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EarI EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI