################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AclI AflIII AgeI Alw44I ApaLI AscI AseI AsnI AvrII BbeI BbvCI BcgI BciVI BglI BglII BlnI BsaBI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraIII EcoO65I EcoRV FseI HindIII HpaI KasI KspI MamI MfeI MluI MunI NarI NcoI NdeI NheI NotI NruI NspI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 483 GACGTC GACGT^C Acc65I 1549 GGTACC G^GTACC AccII 871 CGCG CG^CG AccIII 2188 TCCGGA T^CCGGA AflII 831 CTTAAG C^TTAAG AhdI 2905 GACCTTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 87 GGGCCC GGGCC^C Asp718I 1549 GGTACC G^GTACC AspEI 2905 GACCTTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1746 GAGCTC G[AT]GC[AT]^C AspI 1254 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1135 TGCGCA TGC^GCA BamHI 1129 GGATCC G^GATCC BbrPI 1056 CACGTG CAC^GTG BfrI 831 CTTAAG C^TTAAG BsaI 1269 GAGACC GGTCTC(1/5) BsaWI 2188 TCCGGA [AT]^CCGG[AT] BseAI 2188 TCCGGA T^CCGGA BsiHKAI 1746 GAGCTC G[AT]GC[AT]^C BsmI 2048 GAATGC GAATGC(1/-1) BspEI 2188 TCCGGA T^CCGGA BssSI 531 CACGAG CACGAG(-5/-1) BstAPI 530 GCACGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 871 CGCG CG^CG BstXI 328 CCATGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 2554 TTTAAA TTT^AAA DsaI 1690 CCGTGG C^C[GA][CT]GG Eam1105I 2905 GACCTTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 683 CCTATGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 2660 ATGCAT ATGCA^T FspI 1135 TGCGCA TGC^GCA KpnI 1549 GGTACC GGTAC^C MroI 2188 TCCGGA T^CCGGA MvnI 871 CGCG CG^CG NsiI 2660 ATGCAT ATGCA^T PflFI 1254 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1056 CACGTG CAC^GTG PmlI 1056 CACGTG CAC^GTG SacI 1746 GAGCTC GAGCT^C SalI 1260 GTCGAC G^TCGAC SapI 1725 GAAGAGC GCTCTTC(1/4) Tth111I 1254 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 2270 CCATCCTTTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AccI 1260 GTCGAC GT^[AC][GT]AC 2855 GTCTAC AcsI 1061 GAATTC [GA]^AATT[CT] 2573 GAATTC AcyI 234 GACGCC G[GA]^CG[CT]C 483 GACGTC Aor51HI 379 AGCGCT AGC^GCT 1771 AGCGCT ApoI 1061 GAATTC [GA]^AATT[CT] 2573 GAATTC AvaI 54 CCCGAG C^[CT]CG[GA]G 102 CCCGAG BbsI 1985 GAAGAC GAAGAC(2/6) 2763 GTCTTC BclI 2370 TGATCA T^GATCA 2595 TGATCA BlpI 825 GCTGAGC GC^T[ACGT]AGC 2870 GCTAAGC Bpu1102I 825 GCTGAGC GC^T[ACGT]AGC 2870 GCTAAGC BpuAI 1985 GAAGAC GAAGAC(2/6) 2763 GTCTTC BsaAI 1056 CACGTG [CT]AC^GT[GA] 2257 CACGTA BsaHI 234 GACGCC G[GA]^CG[CT]C 483 GACGTC BsiEI 776 CGGCCG CG[GA][CT]^CG 857 CGGCCG BsoBI 54 CCCGAG C^[CT]CG[GA]G 102 CCCGAG BsrDI 1864 GCAATG GCAATG(2/0) 2247 GCAATG BsrFI 631 GCCGGC [GA]^CCGG[CT] 855 GCCGGC BstYI 669 AGATCC [GA]^GATC[CT] 1129 GGATCC CelII 825 GCTGAGC GC^T[ACGT]AGC 2870 GCTAAGC Cfr10I 631 GCCGGC [GA]^CCGG[CT] 855 GCCGGC DrdI 1464 GACCCGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2194 GACAGAATCGTC EagI 776 CGGCCG C^GGCCG 857 CGGCCG EclXI 776 CGGCCG C^GGCCG 857 CGGCCG Eco47III 379 AGCGCT AGC^GCT 1771 AGCGCT Eco52I 776 CGGCCG C^GGCCG 857 CGGCCG EcoRI 1061 GAATTC G^AATTC 2573 GAATTC FbaI 2370 TGATCA T^GATCA 2595 TGATCA Hin1I 234 GACGCC G[GA]^CG[CT]C 483 GACGTC HincII 1260 GTCGAC GT[CT]^[GA]AC 1890 GTCAAC HindII 1260 GTCGAC GT[CT]^[GA]AC 1890 GTCAAC MflI 669 AGATCC [GA]^GATC[CT] 1129 GGATCC NaeI 631 GCCGGC GCC^GGC 855 GCCGGC NgoMIV 631 GCCGGC G^CCGGC 855 GCCGGC SexAI 334 ACCTGGT A^CC[AT]GGT 1552 ACCTGGT SmlI 831 CTTAAG C^T[CT][GA]AG 1749 CTCAAG StuI 322 AGGCCT AGG^CCT 1675 AGGCCT Tsp509I 1062 AATT ^AATT 2574 AATT XhoII 669 AGATCC [GA]^GATC[CT] 1129 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AocI Asp700I AvaII BalI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I Van91I XmnI