##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AclI AflIII AgeI Alw44I ApaLI
AscI AseI AsnI AvrII BbeI
BbvCI BcgI BciVI BglI BglII
BlnI BsaBI BsiWI BsmBI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraIII EcoO65I
EcoRV FseI HindIII HpaI KasI
KspI MamI MfeI MluI MunI
NarI NcoI NdeI NheI NotI
NruI NspI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 483 GACGTC GACGT^C
Acc65I 1549 GGTACC G^GTACC
AccII 871 CGCG CG^CG
AccIII 2188 TCCGGA T^CCGGA
AflII 831 CTTAAG C^TTAAG
AhdI 2905 GACCTTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 87 GGGCCC GGGCC^C
Asp718I 1549 GGTACC G^GTACC
AspEI 2905 GACCTTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1746 GAGCTC G[AT]GC[AT]^C
AspI 1254 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1135 TGCGCA TGC^GCA
BamHI 1129 GGATCC G^GATCC
BbrPI 1056 CACGTG CAC^GTG
BfrI 831 CTTAAG C^TTAAG
BsaI 1269 GAGACC GGTCTC(1/5)
BsaWI 2188 TCCGGA [AT]^CCGG[AT]
BseAI 2188 TCCGGA T^CCGGA
BsiHKAI 1746 GAGCTC G[AT]GC[AT]^C
BsmI 2048 GAATGC GAATGC(1/-1)
BspEI 2188 TCCGGA T^CCGGA
BssSI 531 CACGAG CACGAG(-5/-1)
BstAPI 530 GCACGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 871 CGCG CG^CG
BstXI 328 CCATGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 2554 TTTAAA TTT^AAA
DsaI 1690 CCGTGG C^C[GA][CT]GG
Eam1105I 2905 GACCTTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 683 CCTATGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2660 ATGCAT ATGCA^T
FspI 1135 TGCGCA TGC^GCA
KpnI 1549 GGTACC GGTAC^C
MroI 2188 TCCGGA T^CCGGA
MvnI 871 CGCG CG^CG
NsiI 2660 ATGCAT ATGCA^T
PflFI 1254 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1056 CACGTG CAC^GTG
PmlI 1056 CACGTG CAC^GTG
SacI 1746 GAGCTC GAGCT^C
SalI 1260 GTCGAC G^TCGAC
SapI 1725 GAAGAGC GCTCTTC(1/4)
Tth111I 1254 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2270 CCATCCTTTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccI 1260 GTCGAC GT^[AC][GT]AC
2855 GTCTAC
AcsI 1061 GAATTC [GA]^AATT[CT]
2573 GAATTC
AcyI 234 GACGCC G[GA]^CG[CT]C
483 GACGTC
Aor51HI 379 AGCGCT AGC^GCT
1771 AGCGCT
ApoI 1061 GAATTC [GA]^AATT[CT]
2573 GAATTC
AvaI 54 CCCGAG C^[CT]CG[GA]G
102 CCCGAG
BbsI 1985 GAAGAC GAAGAC(2/6)
2763 GTCTTC
BclI 2370 TGATCA T^GATCA
2595 TGATCA
BlpI 825 GCTGAGC GC^T[ACGT]AGC
2870 GCTAAGC
Bpu1102I 825 GCTGAGC GC^T[ACGT]AGC
2870 GCTAAGC
BpuAI 1985 GAAGAC GAAGAC(2/6)
2763 GTCTTC
BsaAI 1056 CACGTG [CT]AC^GT[GA]
2257 CACGTA
BsaHI 234 GACGCC G[GA]^CG[CT]C
483 GACGTC
BsiEI 776 CGGCCG CG[GA][CT]^CG
857 CGGCCG
BsoBI 54 CCCGAG C^[CT]CG[GA]G
102 CCCGAG
BsrDI 1864 GCAATG GCAATG(2/0)
2247 GCAATG
BsrFI 631 GCCGGC [GA]^CCGG[CT]
855 GCCGGC
BstYI 669 AGATCC [GA]^GATC[CT]
1129 GGATCC
CelII 825 GCTGAGC GC^T[ACGT]AGC
2870 GCTAAGC
Cfr10I 631 GCCGGC [GA]^CCGG[CT]
855 GCCGGC
DrdI 1464 GACCCGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2194 GACAGAATCGTC
EagI 776 CGGCCG C^GGCCG
857 CGGCCG
EclXI 776 CGGCCG C^GGCCG
857 CGGCCG
Eco47III 379 AGCGCT AGC^GCT
1771 AGCGCT
Eco52I 776 CGGCCG C^GGCCG
857 CGGCCG
EcoRI 1061 GAATTC G^AATTC
2573 GAATTC
FbaI 2370 TGATCA T^GATCA
2595 TGATCA
Hin1I 234 GACGCC G[GA]^CG[CT]C
483 GACGTC
HincII 1260 GTCGAC GT[CT]^[GA]AC
1890 GTCAAC
HindII 1260 GTCGAC GT[CT]^[GA]AC
1890 GTCAAC
MflI 669 AGATCC [GA]^GATC[CT]
1129 GGATCC
NaeI 631 GCCGGC GCC^GGC
855 GCCGGC
NgoMIV 631 GCCGGC G^CCGGC
855 GCCGGC
SexAI 334 ACCTGGT A^CC[AT]GGT
1552 ACCTGGT
SmlI 831 CTTAAG C^T[CT][GA]AG
1749 CTCAAG
StuI 322 AGGCCT AGG^CCT
1675 AGGCCT
Tsp509I 1062 AATT ^AATT
2574 AATT
XhoII 669 AGATCC [GA]^GATC[CT]
1129 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI Asp700I AvaII BalI BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I Van91I XmnI