##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

AclI        AflIII      AgeI        Alw44I      ApaLI       
AscI        AseI        AsnI        AvrII       BbeI        
BbvCI       BcgI        BciVI       BglI        BglII       
BlnI        BsaBI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspHI       BspLU11I    BsrBI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     ClaI        CpoI        DraIII      EcoO65I     
EcoRV       FseI        HindIII     HpaI        KasI        
KspI        MamI        MfeI        MluI        MunI        
NarI        NcoI        NdeI        NheI        NotI        
NruI        NspI        NspV        PacI        PaeR7I      
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AatII         483  GACGTC               GACGT^C
Acc65I       1549  GGTACC               G^GTACC
AccII         871  CGCG                 CG^CG
AccIII       2188  TCCGGA               T^CCGGA
AflII         831  CTTAAG               C^TTAAG
AhdI         2905  GACCTTAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI           87  GGGCCC               GGGCC^C
Asp718I      1549  GGTACC               G^GTACC
AspEI        2905  GACCTTAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1746  GAGCTC               G[AT]GC[AT]^C
AspI         1254  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1135  TGCGCA               TGC^GCA
BamHI        1129  GGATCC               G^GATCC
BbrPI        1056  CACGTG               CAC^GTG
BfrI          831  CTTAAG               C^TTAAG
BsaI         1269  GAGACC               GGTCTC(1/5)
BsaWI        2188  TCCGGA               [AT]^CCGG[AT]
BseAI        2188  TCCGGA               T^CCGGA
BsiHKAI      1746  GAGCTC               G[AT]GC[AT]^C
BsmI         2048  GAATGC               GAATGC(1/-1)
BspEI        2188  TCCGGA               T^CCGGA
BssSI         531  CACGAG               CACGAG(-5/-1)
BstAPI        530  GCACGAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         871  CGCG                 CG^CG
BstXI         328  CCATGCACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI         2554  TTTAAA               TTT^AAA
DsaI         1690  CCGTGG               C^C[GA][CT]GG
Eam1105I     2905  GACCTTAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         683  CCTATGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I      2660  ATGCAT               ATGCA^T
FspI         1135  TGCGCA               TGC^GCA
KpnI         1549  GGTACC               GGTAC^C
MroI         2188  TCCGGA               T^CCGGA
MvnI          871  CGCG                 CG^CG
NsiI         2660  ATGCAT               ATGCA^T
PflFI        1254  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1056  CACGTG               CAC^GTG
PmlI         1056  CACGTG               CAC^GTG
SacI         1746  GAGCTC               GAGCT^C
SalI         1260  GTCGAC               G^TCGAC
SapI         1725  GAAGAGC              GCTCTTC(1/4)
Tth111I      1254  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         2270  CCATCCTTTCCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   38 enzymes) #
###################################

AccI         1260  GTCGAC               GT^[AC][GT]AC
             2855  GTCTAC              
AcsI         1061  GAATTC               [GA]^AATT[CT]
             2573  GAATTC              
AcyI          234  GACGCC               G[GA]^CG[CT]C
              483  GACGTC              
Aor51HI       379  AGCGCT               AGC^GCT
             1771  AGCGCT              
ApoI         1061  GAATTC               [GA]^AATT[CT]
             2573  GAATTC              
AvaI           54  CCCGAG               C^[CT]CG[GA]G
              102  CCCGAG              
BbsI         1985  GAAGAC               GAAGAC(2/6)
             2763  GTCTTC              
BclI         2370  TGATCA               T^GATCA
             2595  TGATCA              
BlpI          825  GCTGAGC              GC^T[ACGT]AGC
             2870  GCTAAGC             
Bpu1102I      825  GCTGAGC              GC^T[ACGT]AGC
             2870  GCTAAGC             
BpuAI        1985  GAAGAC               GAAGAC(2/6)
             2763  GTCTTC              
BsaAI        1056  CACGTG               [CT]AC^GT[GA]
             2257  CACGTA              
BsaHI         234  GACGCC               G[GA]^CG[CT]C
              483  GACGTC              
BsiEI         776  CGGCCG               CG[GA][CT]^CG
              857  CGGCCG              
BsoBI          54  CCCGAG               C^[CT]CG[GA]G
              102  CCCGAG              
BsrDI        1864  GCAATG               GCAATG(2/0)
             2247  GCAATG              
BsrFI         631  GCCGGC               [GA]^CCGG[CT]
              855  GCCGGC              
BstYI         669  AGATCC               [GA]^GATC[CT]
             1129  GGATCC              
CelII         825  GCTGAGC              GC^T[ACGT]AGC
             2870  GCTAAGC             
Cfr10I        631  GCCGGC               [GA]^CCGG[CT]
              855  GCCGGC              
DrdI         1464  GACCCGCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2194  GACAGAATCGTC        
EagI          776  CGGCCG               C^GGCCG
              857  CGGCCG              
EclXI         776  CGGCCG               C^GGCCG
              857  CGGCCG              
Eco47III      379  AGCGCT               AGC^GCT
             1771  AGCGCT              
Eco52I        776  CGGCCG               C^GGCCG
              857  CGGCCG              
EcoRI        1061  GAATTC               G^AATTC
             2573  GAATTC              
FbaI         2370  TGATCA               T^GATCA
             2595  TGATCA              
Hin1I         234  GACGCC               G[GA]^CG[CT]C
              483  GACGTC              
HincII       1260  GTCGAC               GT[CT]^[GA]AC
             1890  GTCAAC              
HindII       1260  GTCGAC               GT[CT]^[GA]AC
             1890  GTCAAC              
MflI          669  AGATCC               [GA]^GATC[CT]
             1129  GGATCC              
NaeI          631  GCCGGC               GCC^GGC
              855  GCCGGC              
NgoMIV        631  GCCGGC               G^CCGGC
              855  GCCGGC              
SexAI         334  ACCTGGT              A^CC[AT]GGT
             1552  ACCTGGT             
SmlI          831  CTTAAG               C^T[CT][GA]AG
             1749  CTCAAG              
StuI          322  AGGCCT               AGG^CCT
             1675  AGGCCT              
Tsp509I      1062  AATT                 ^AATT
             2574  AATT                
XhoII         669  AGATCC               [GA]^GATC[CT]
             1129  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AocI        Asp700I     AvaII       BalI        BanI        
BanII       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaJI       BseRI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        Eco81I      EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      TspRI       TthHB8I     Van91I      XmnI