##################################
# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI Alw44I
AlwNI ApaI ApaLI AscI AseI
AsnI Asp718I AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DraII DraIII DrdI
DsaI EaeI EagI EclXI Eco52I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FbaI FseI FspI HapII
HgaI Hin1I HpaII KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NlaIV NotI NruI NsiI NspI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacII
SalI SapI SbfI SexAI SfcI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI StyI
Tsp45I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AflII 2208 CTTAAG C^TTAAG
AhdI 210 GACACATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2633 CCTCAGG CC^T[ACGT]AGG
Aor51HI 629 AGCGCT AGC^GCT
AspEI 210 GACACATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2406 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 850 CTCGGG C^[CT]CG[GA]G
AvaII 2748 GGACC G^G[AT]CC
BanII 2537 GAGCTC G[GA]GC[CT]^C
BfrI 2208 CTTAAG C^TTAAG
BsaI 807 GGTCTC GGTCTC(1/5)
BsoBI 850 CTCGGG C^[CT]CG[GA]G
Bsp1407I 3001 TGTACA T^GTACA
BsrGI 3001 TGTACA T^GTACA
BstBI 1569 TTCGAA TT^CGAA
Bsu36I 2633 CCTCAGG CC^T[ACGT]AGG
Cfr13I 2748 GGACC G^G[ACGT]CC
Eam1105I 210 GACACATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 629 AGCGCT AGC^GCT
Eco81I 2633 CCTCAGG CC^T[ACGT]AGG
EcoRI 1347 GAATTC G^AATTC
FauI 503 GCGGG CCCGC(4/6)
HaeII 629 AGCGCT [GA]GCGC^[CT]
HindIII 1249 AAGCTT A^AGCTT
MspA1I 359 CCGCTG C[AC]G^C[GT]G
NheI 2545 GCTAGC G^CTAGC
NspV 1569 TTCGAA TT^CGAA
PflFI 2406 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 2537 GAGCTC GAGCT^C
Sau96I 2748 GGACC G^G[ACGT]CC
SfuI 1569 TTCGAA TT^CGAA
SspBI 3001 TGTACA T^GTACA
Tth111I 2406 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwI 1484 GATCC GGATC(4/5)
518 GGATC
Asp700I 347 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2265 GAACATTTTC
AspHI 2386 GTGCTC G[AT]GC[AT]^C
2537 GAGCTC
BglII 1455 AGATCT A^GATCT
1552 AGATCT
BmyI 2386 GTGCTC G[AGT]GC[ACT]^C
2537 GAGCTC
BsiHKAI 2386 GTGCTC G[AT]GC[AT]^C
2537 GAGCTC
BsmAI 2863 GAGAC GTCTC(1/5)
808 GTCTC
Bsp1286I 2386 GTGCTC G[AGT]GC[ACT]^C
2537 GAGCTC
BspHI 1004 TCATGA T^CATGA
2397 TCATGA
BspMI 2942 ACCTGC ACCTGC(4/8)
1814 GCAGGT
BstYI 1455 AGATCT [GA]^GATC[CT]
1552 AGATCT
CfoI 630 GCGC GCG^C
2980 GCGC
HhaI 630 GCGC GCG^C
2980 GCGC
HinP1I 630 GCGC G^CGC
2980 GCGC
HpaI 1443 GTTAAC GTT^AAC
1640 GTTAAC
MaeII 770 ACGT A^CGT
1188 ACGT
MflI 1455 AGATCT [GA]^GATC[CT]
1552 AGATCT
MslI 131 CACAGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
746 CATTTTAATG
MwoI 1985 GCGTGGTAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
2982 GCCACCACCGC
NdeI 786 CATATG CA^TATG
2997 CATATG
PflMI 2957 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2986 CCACCGCCTGG
RcaI 1004 TCATGA T^CATGA
2397 TCATGA
ScaI 1759 AGTACT AGT^ACT
1854 AGTACT
SfaNI 686 GATGC GCATC(5/9)
1029 GCATC
SwaI 997 ATTTAAAT ATTT^AAAT
1431 ATTTAAAT
Van91I 2957 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2986 CCACCGCCTGG
XhoII 1455 AGATCT [GA]^GATC[CT]
1552 AGATCT
XmnI 347 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2265 GAACATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbsI BbvI BfaI BpmI BpuAI
BsaJI BsiYI BslI BsmFI BsrDI
BsrI BssKI BstF5I BstNI Cac8I
DdeI DpnII DraI EarI EcoRII
Fnu4HI FokI HaeIII HincII HindII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MvaI NdeII NlaIII PleI RsaI
Sau3AI ScrFI SmlI SspI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I