################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflIII AgeI Alw44I AlwNI ApaI ApaLI AscI AseI AsnI Asp718I AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI EclXI Eco52I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HapII HgaI Hin1I HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NlaIV NotI NruI NsiI NspI PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI StyI Tsp45I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AflII 2208 CTTAAG C^TTAAG AhdI 210 GACACATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2633 CCTCAGG CC^T[ACGT]AGG Aor51HI 629 AGCGCT AGC^GCT AspEI 210 GACACATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2406 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 850 CTCGGG C^[CT]CG[GA]G AvaII 2748 GGACC G^G[AT]CC BanII 2537 GAGCTC G[GA]GC[CT]^C BfrI 2208 CTTAAG C^TTAAG BsaI 807 GGTCTC GGTCTC(1/5) BsoBI 850 CTCGGG C^[CT]CG[GA]G Bsp1407I 3001 TGTACA T^GTACA BsrGI 3001 TGTACA T^GTACA BstBI 1569 TTCGAA TT^CGAA Bsu36I 2633 CCTCAGG CC^T[ACGT]AGG Cfr13I 2748 GGACC G^G[ACGT]CC Eam1105I 210 GACACATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 629 AGCGCT AGC^GCT Eco81I 2633 CCTCAGG CC^T[ACGT]AGG EcoRI 1347 GAATTC G^AATTC FauI 503 GCGGG CCCGC(4/6) HaeII 629 AGCGCT [GA]GCGC^[CT] HindIII 1249 AAGCTT A^AGCTT MspA1I 359 CCGCTG C[AC]G^C[GT]G NheI 2545 GCTAGC G^CTAGC NspV 1569 TTCGAA TT^CGAA PflFI 2406 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC SacI 2537 GAGCTC GAGCT^C Sau96I 2748 GGACC G^G[ACGT]CC SfuI 1569 TTCGAA TT^CGAA SspBI 3001 TGTACA T^GTACA Tth111I 2406 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AlwI 1484 GATCC GGATC(4/5) 518 GGATC Asp700I 347 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2265 GAACATTTTC AspHI 2386 GTGCTC G[AT]GC[AT]^C 2537 GAGCTC BglII 1455 AGATCT A^GATCT 1552 AGATCT BmyI 2386 GTGCTC G[AGT]GC[ACT]^C 2537 GAGCTC BsiHKAI 2386 GTGCTC G[AT]GC[AT]^C 2537 GAGCTC BsmAI 2863 GAGAC GTCTC(1/5) 808 GTCTC Bsp1286I 2386 GTGCTC G[AGT]GC[ACT]^C 2537 GAGCTC BspHI 1004 TCATGA T^CATGA 2397 TCATGA BspMI 2942 ACCTGC ACCTGC(4/8) 1814 GCAGGT BstYI 1455 AGATCT [GA]^GATC[CT] 1552 AGATCT CfoI 630 GCGC GCG^C 2980 GCGC HhaI 630 GCGC GCG^C 2980 GCGC HinP1I 630 GCGC G^CGC 2980 GCGC HpaI 1443 GTTAAC GTT^AAC 1640 GTTAAC MaeII 770 ACGT A^CGT 1188 ACGT MflI 1455 AGATCT [GA]^GATC[CT] 1552 AGATCT MslI 131 CACAGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 746 CATTTTAATG MwoI 1985 GCGTGGTAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 2982 GCCACCACCGC NdeI 786 CATATG CA^TATG 2997 CATATG PflMI 2957 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2986 CCACCGCCTGG RcaI 1004 TCATGA T^CATGA 2397 TCATGA ScaI 1759 AGTACT AGT^ACT 1854 AGTACT SfaNI 686 GATGC GCATC(5/9) 1029 GCATC SwaI 997 ATTTAAAT ATTT^AAAT 1431 ATTTAAAT Van91I 2957 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2986 CCACCGCCTGG XhoII 1455 AGATCT [GA]^GATC[CT] 1552 AGATCT XmnI 347 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2265 GAACATTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI BbsI BbvI BfaI BpmI BpuAI BsaJI BsiYI BslI BsmFI BsrDI BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII DraI EarI EcoRII Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MvaI NdeII NlaIII PleI RsaI Sau3AI ScrFI SmlI SspI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I