##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccII AcyI AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BciVI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseRI BsgI BsiWI BsmBI
BsoBI BspDI BspMI BsrFI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FauI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AclI 2434 AACGTT AA^CGTT
AflII 1969 CTTAAG C^TTAAG
AlwNI 350 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 736 TGGCCA TGG^CCA
BanI 1757 GGTGCC G^G[CT][GA]CC
BcgI 1318 GCACACAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2730 TGATCA T^GATCA
BfrI 1969 CTTAAG C^TTAAG
BsiEI 1620 CGGTCG CG[GA][CT]^CG
BsmFI 898 GTCCC GGGAC(10/14)
BsmI 698 GAATGC GAATGC(1/-1)
Bsp1407I 2395 TGTACA T^GTACA
BsrBI 2299 CCGCTC CCGCTC(-3/-3)
BsrDI 1457 GCAATG GCAATG(2/0)
BsrGI 2395 TGTACA T^GTACA
BstBI 2520 TTCGAA TT^CGAA
BstXI 196 CCAGGTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 848 GCGC GCG^C
DraII 2494 GGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 1471 GACTGCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 928 CCATGG C^C[GA][CT]GG
EcoO109I 2494 GGGTCCT [GA]G^G[ACGT]CC[CT]
FbaI 2730 TGATCA T^GATCA
HaeII 847 GGCGCT [GA]GCGC^[CT]
HhaI 848 GCGC GCG^C
HinP1I 848 GCGC G^CGC
MluNI 736 TGGCCA TGG^CCA
MscI 736 TGGCCA TGG^CCA
NcoI 928 CCATGG C^CATGG
NspV 2520 TTCGAA TT^CGAA
PflMI 324 CCACAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2494 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 2434 AACGTT AA^CGTT
SacI 585 GAGCTC GAGCT^C
SapI 2523 GAAGAGC GCTCTTC(1/4)
ScaI 1065 AGTACT AGT^ACT
SfuI 2520 TTCGAA TT^CGAA
SspBI 2395 TGTACA T^GTACA
Van91I 324 CCACAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1428 CCAAGGTATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 181 GTCTAC GT^[AC][GT]AC
1932 GTAGAC
AccIII 642 TCCGGA T^CCGGA
2446 TCCGGA
BanII 585 GAGCTC G[GA]GC[CT]^C
1870 GGGCTC
BbvCI 1448 CCTCAGC CCTCAGC(-5/-2)
76 GCTGAGG
BcnI 934 CCGGG CC^[GC]GG
2664 CCGGG
BglII 1154 AGATCT A^GATCT
2285 AGATCT
BsaI 2083 GAGACC GGTCTC(1/5)
2346 GAGACC
BsaWI 642 TCCGGA [AT]^CCGG[AT]
2446 TCCGGA
BseAI 642 TCCGGA T^CCGGA
2446 TCCGGA
BspEI 642 TCCGGA T^CCGGA
2446 TCCGGA
DraI 1661 TTTAAA TTT^AAA
1864 TTTAAA
EcoRI 607 GAATTC G^AATTC
1943 GAATTC
MroI 642 TCCGGA T^CCGGA
2446 TCCGGA
MspA1I 176 CAGCTG C[AC]G^C[GT]G
438 CAGCTG
NciI 934 CCGGG CC^[GC]GG
2664 CCGGG
NdeI 1541 CATATG CA^TATG
2016 CATATG
PstI 1718 CTGCAG CTGCA^G
2307 CTGCAG
PvuII 176 CAGCTG CAG^CTG
438 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI AvaII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BspHI BspLU11I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EaeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI