################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccII AcyI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BciVI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseRI BsgI BsiWI BsmBI BsoBI BspDI BspMI BsrFI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FauI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AclI 2434 AACGTT AA^CGTT AflII 1969 CTTAAG C^TTAAG AlwNI 350 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG BalI 736 TGGCCA TGG^CCA BanI 1757 GGTGCC G^G[CT][GA]CC BcgI 1318 GCACACAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2730 TGATCA T^GATCA BfrI 1969 CTTAAG C^TTAAG BsiEI 1620 CGGTCG CG[GA][CT]^CG BsmFI 898 GTCCC GGGAC(10/14) BsmI 698 GAATGC GAATGC(1/-1) Bsp1407I 2395 TGTACA T^GTACA BsrBI 2299 CCGCTC CCGCTC(-3/-3) BsrDI 1457 GCAATG GCAATG(2/0) BsrGI 2395 TGTACA T^GTACA BstBI 2520 TTCGAA TT^CGAA BstXI 196 CCAGGTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 848 GCGC GCG^C DraII 2494 GGGTCCT [GA]G^G[ACGT]CC[CT] DrdI 1471 GACTGCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 928 CCATGG C^C[GA][CT]GG EcoO109I 2494 GGGTCCT [GA]G^G[ACGT]CC[CT] FbaI 2730 TGATCA T^GATCA HaeII 847 GGCGCT [GA]GCGC^[CT] HhaI 848 GCGC GCG^C HinP1I 848 GCGC G^CGC MluNI 736 TGGCCA TGG^CCA MscI 736 TGGCCA TGG^CCA NcoI 928 CCATGG C^CATGG NspV 2520 TTCGAA TT^CGAA PflMI 324 CCACAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 2494 GGGTCCT [GA]G^G[AT]CC[CT] Psp1406I 2434 AACGTT AA^CGTT SacI 585 GAGCTC GAGCT^C SapI 2523 GAAGAGC GCTCTTC(1/4) ScaI 1065 AGTACT AGT^ACT SfuI 2520 TTCGAA TT^CGAA SspBI 2395 TGTACA T^GTACA Van91I 324 CCACAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1428 CCAAGGTATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccI 181 GTCTAC GT^[AC][GT]AC 1932 GTAGAC AccIII 642 TCCGGA T^CCGGA 2446 TCCGGA BanII 585 GAGCTC G[GA]GC[CT]^C 1870 GGGCTC BbvCI 1448 CCTCAGC CCTCAGC(-5/-2) 76 GCTGAGG BcnI 934 CCGGG CC^[GC]GG 2664 CCGGG BglII 1154 AGATCT A^GATCT 2285 AGATCT BsaI 2083 GAGACC GGTCTC(1/5) 2346 GAGACC BsaWI 642 TCCGGA [AT]^CCGG[AT] 2446 TCCGGA BseAI 642 TCCGGA T^CCGGA 2446 TCCGGA BspEI 642 TCCGGA T^CCGGA 2446 TCCGGA DraI 1661 TTTAAA TTT^AAA 1864 TTTAAA EcoRI 607 GAATTC G^AATTC 1943 GAATTC MroI 642 TCCGGA T^CCGGA 2446 TCCGGA MspA1I 176 CAGCTG C[AC]G^C[GT]G 438 CAGCTG NciI 934 CCGGG CC^[GC]GG 2664 CCGGG NdeI 1541 CATATG CA^TATG 2016 CATATG PstI 1718 CTGCAG CTGCA^G 2307 CTGCAG PvuII 176 CAGCTG CAG^CTG 438 CAGCTG ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI Asp700I AspHI AvaII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspHI BspLU11I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI