##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AclI AflII AhdI Aor51HI AscI
AseI AsnI AspEI AviII AvrII
BbrPI BfrI BglI BglII BlnI
BsaBI BsiEI BsiWI BsmI BspDI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HpaI MamI
MfeI MluI MunI NaeI NgoMIV
NotI NruI NsiI NspV PacI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SalI SbfI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 670 GACGTC GACGT^C
AcsI 34 GAATTT [GA]^AATT[CT]
AgeI 2888 ACCGGT A^CCGGT
ApoI 34 GAATTT [GA]^AATT[CT]
BamHI 2896 GGATCC G^GATCC
BbeI 72 GGCGCC GGCGC^C
BclI 1776 TGATCA T^GATCA
BlpI 2760 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2760 GCTCAGC GC^T[ACGT]AGC
BsaAI 1058 CACGTA [CT]AC^GT[GA]
BsmBI 378 CGTCTC CGTCTC(1/5)
Bsp1407I 2582 TGTACA T^GTACA
BsrDI 2288 CATTGC GCAATG(2/0)
BsrGI 2582 TGTACA T^GTACA
BssSI 381 CTCGTG CACGAG(-5/-1)
BstEII 1414 GGTTACC G^GT[ACGT]ACC
BstPI 1414 GGTTACC G^GT[ACGT]ACC
CelII 2760 GCTCAGC GC^T[ACGT]AGC
DraI 637 TTTAAA TTT^AAA
DrdI 1047 GACTCAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1414 GGTTACC G^GT[ACGT]ACC
FbaI 1776 TGATCA T^GATCA
HindIII 344 AAGCTT A^AGCTT
KasI 72 GGCGCC G^GCGCC
KspI 55 CCGCGG CCGC^GG
NarI 72 GGCGCC GG^CGCC
NdeI 1150 CATATG CA^TATG
NheI 2745 GCTAGC G^CTAGC
PaeR7I 2500 CTCGAG C^TCGAG
PinAI 2888 ACCGGT A^CCGGT
PpuMI 2588 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 802 GACGCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 55 CCGCGG CCGC^GG
SapI 924 GAAGAGC GCTCTTC(1/4)
ScaI 2841 AGTACT AGT^ACT
SexAI 11 ACCTGGT A^CC[AT]GGT
SfoI 72 GGCGCC GGC^GCC
SphI 1022 GCATGC GCATG^C
SspBI 2582 TGTACA T^GTACA
XbaI 2700 TCTAGA T^CTAGA
XhoI 2500 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 764 GGTACC G^GTACC
885 GGTACC
AccII 56 CGCG CG^CG
1300 CGCG
AccIII 143 TCCGGA T^CCGGA
1253 TCCGGA
AflIII 642 ACATGT A^C[GA][CT]GT
1232 ACATGT
Alw44I 2926 GTGCAC G^TGCAC
3373 GTGCAC
ApaI 1441 GGGCCC GGGCC^C
2463 GGGCCC
ApaLI 2926 GTGCAC G^TGCAC
3373 GTGCAC
Asp700I 290 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2026 GAACCAGTTC
Asp718I 764 GGTACC G^GTACC
885 GGTACC
AspI 1593 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2827 GACAGGGTC
AvaI 2500 CTCGAG C^[CT]CG[GA]G
2611 CTCGGG
BbsI 667 GAAGAC GAAGAC(2/6)
1590 GAAGAC
BbvCI 1740 CCTCAGC CCTCAGC(-5/-2)
2296 CCTCAGC
BciVI 3363 GGATAC GTATCC(6/5)
422 GTATCC
BpuAI 667 GAAGAC GAAGAC(2/6)
1590 GAAGAC
BseAI 143 TCCGGA T^CCGGA
1253 TCCGGA
BsoBI 2500 CTCGAG C^[CT]CG[GA]G
2611 CTCGGG
BspEI 143 TCCGGA T^CCGGA
1253 TCCGGA
BspLU11I 642 ACATGT A^CATGT
1232 ACATGT
BsrBI 276 CCGCTC CCGCTC(-3/-3)
2704 GAGCGG
BsrFI 1834 ACCGGC [GA]^CCGG[CT]
2888 ACCGGT
BstAPI 2283 GCACTCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2868 GCAGCCCATGC
BstUI 56 CGCG CG^CG
1300 CGCG
Cfr10I 1834 ACCGGC [GA]^CCGG[CT]
2888 ACCGGT
HaeII 72 GGCGCC [GA]GCGC^[CT]
3330 AGCGCC
KpnI 764 GGTACC GGTAC^C
885 GGTACC
MaeII 671 ACGT A^CGT
1059 ACGT
MroI 143 TCCGGA T^CCGGA
1253 TCCGGA
MvnI 56 CGCG CG^CG
1300 CGCG
NcoI 1644 CCATGG C^CATGG
2326 CCATGG
PflFI 1593 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2827 GACAGGGTC
PflMI 851 CCATTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1170 CCACCCAGTGG
SacI 1531 GAGCTC GAGCT^C
1828 GAGCTC
SmlI 2005 CTTGAG C^T[CT][GA]AG
2500 CTCGAG
StuI 2920 AGGCCT AGG^CCT
3230 AGGCCT
Tth111I 1593 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2827 GACAGGGTC
Van91I 851 CCATTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1170 CCACCCAGTGG
XmnI 290 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2026 GAACCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AciI AcyI AfaI AluI
AlwI AlwNI AocI AspHI AvaII
BalI BanI BanII BbvI BcgI
BcnI BfaI BmyI BpmI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BspMI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII