################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AclI AflII AhdI Aor51HI AscI AseI AsnI AspEI AviII AvrII BbrPI BfrI BglI BglII BlnI BsaBI BsiEI BsiWI BsmI BspDI BssHII Bst1107I BstBI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HpaI MamI MfeI MluI MunI NaeI NgoMIV NotI NruI NsiI NspV PacI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 670 GACGTC GACGT^C AcsI 34 GAATTT [GA]^AATT[CT] AgeI 2888 ACCGGT A^CCGGT ApoI 34 GAATTT [GA]^AATT[CT] BamHI 2896 GGATCC G^GATCC BbeI 72 GGCGCC GGCGC^C BclI 1776 TGATCA T^GATCA BlpI 2760 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2760 GCTCAGC GC^T[ACGT]AGC BsaAI 1058 CACGTA [CT]AC^GT[GA] BsmBI 378 CGTCTC CGTCTC(1/5) Bsp1407I 2582 TGTACA T^GTACA BsrDI 2288 CATTGC GCAATG(2/0) BsrGI 2582 TGTACA T^GTACA BssSI 381 CTCGTG CACGAG(-5/-1) BstEII 1414 GGTTACC G^GT[ACGT]ACC BstPI 1414 GGTTACC G^GT[ACGT]ACC CelII 2760 GCTCAGC GC^T[ACGT]AGC DraI 637 TTTAAA TTT^AAA DrdI 1047 GACTCAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1414 GGTTACC G^GT[ACGT]ACC FbaI 1776 TGATCA T^GATCA HindIII 344 AAGCTT A^AGCTT KasI 72 GGCGCC G^GCGCC KspI 55 CCGCGG CCGC^GG NarI 72 GGCGCC GG^CGCC NdeI 1150 CATATG CA^TATG NheI 2745 GCTAGC G^CTAGC PaeR7I 2500 CTCGAG C^TCGAG PinAI 2888 ACCGGT A^CCGGT PpuMI 2588 GGGACCT [GA]G^G[AT]CC[CT] PshAI 802 GACGCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 55 CCGCGG CCGC^GG SapI 924 GAAGAGC GCTCTTC(1/4) ScaI 2841 AGTACT AGT^ACT SexAI 11 ACCTGGT A^CC[AT]GGT SfoI 72 GGCGCC GGC^GCC SphI 1022 GCATGC GCATG^C SspBI 2582 TGTACA T^GTACA XbaI 2700 TCTAGA T^CTAGA XhoI 2500 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 764 GGTACC G^GTACC 885 GGTACC AccII 56 CGCG CG^CG 1300 CGCG AccIII 143 TCCGGA T^CCGGA 1253 TCCGGA AflIII 642 ACATGT A^C[GA][CT]GT 1232 ACATGT Alw44I 2926 GTGCAC G^TGCAC 3373 GTGCAC ApaI 1441 GGGCCC GGGCC^C 2463 GGGCCC ApaLI 2926 GTGCAC G^TGCAC 3373 GTGCAC Asp700I 290 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2026 GAACCAGTTC Asp718I 764 GGTACC G^GTACC 885 GGTACC AspI 1593 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2827 GACAGGGTC AvaI 2500 CTCGAG C^[CT]CG[GA]G 2611 CTCGGG BbsI 667 GAAGAC GAAGAC(2/6) 1590 GAAGAC BbvCI 1740 CCTCAGC CCTCAGC(-5/-2) 2296 CCTCAGC BciVI 3363 GGATAC GTATCC(6/5) 422 GTATCC BpuAI 667 GAAGAC GAAGAC(2/6) 1590 GAAGAC BseAI 143 TCCGGA T^CCGGA 1253 TCCGGA BsoBI 2500 CTCGAG C^[CT]CG[GA]G 2611 CTCGGG BspEI 143 TCCGGA T^CCGGA 1253 TCCGGA BspLU11I 642 ACATGT A^CATGT 1232 ACATGT BsrBI 276 CCGCTC CCGCTC(-3/-3) 2704 GAGCGG BsrFI 1834 ACCGGC [GA]^CCGG[CT] 2888 ACCGGT BstAPI 2283 GCACTCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2868 GCAGCCCATGC BstUI 56 CGCG CG^CG 1300 CGCG Cfr10I 1834 ACCGGC [GA]^CCGG[CT] 2888 ACCGGT HaeII 72 GGCGCC [GA]GCGC^[CT] 3330 AGCGCC KpnI 764 GGTACC GGTAC^C 885 GGTACC MaeII 671 ACGT A^CGT 1059 ACGT MroI 143 TCCGGA T^CCGGA 1253 TCCGGA MvnI 56 CGCG CG^CG 1300 CGCG NcoI 1644 CCATGG C^CATGG 2326 CCATGG PflFI 1593 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2827 GACAGGGTC PflMI 851 CCATTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1170 CCACCCAGTGG SacI 1531 GAGCTC GAGCT^C 1828 GAGCTC SmlI 2005 CTTGAG C^T[CT][GA]AG 2500 CTCGAG StuI 2920 AGGCCT AGG^CCT 3230 AGGCCT Tth111I 1593 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2827 GACAGGGTC Van91I 851 CCATTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1170 CCACCCAGTGG XmnI 290 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2026 GAACCAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AccI AciI AcyI AfaI AluI AlwI AlwNI AocI AspHI AvaII BalI BanI BanII BbvI BcgI BcnI BfaI BmyI BpmI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII