################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AccIII AcsI AflIII AgeI AhdI Aor51HI ApoI AscI AseI AsnI AspEI BamHI BbeI BbrPI BclI BglII BsaAI BsaBI BseAI BsiWI BsmI BspEI BspHI BspLU11I BssHII BssSI Bst1107I BstAPI BstBI BstZ17I CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI HpaI KasI MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI RcaI RsrII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 516 GTAGAC GT^[AC][GT]AC AclI 2945 AACGTT AA^CGTT AflII 2844 CTTAAG C^TTAAG Alw44I 1398 GTGCAC G^TGCAC ApaLI 1398 GTGCAC G^TGCAC Asp700I 2942 GAAAACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 2409 TGCGCA TGC^GCA AvrII 2921 CCTAGG C^CTAGG BbvCI 2457 CCTCAGC CCTCAGC(-5/-2) BcgI 1988 GCAGCAATATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1967 GTATCC GTATCC(6/5) BfrI 2844 CTTAAG C^TTAAG BlnI 2921 CCTAGG C^CTAGG BlpI 1427 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1427 GCTGAGC GC^T[ACGT]AGC Bsp1407I 2810 TGTACA T^GTACA BsrDI 845 CATTGC GCAATG(2/0) BsrGI 2810 TGTACA T^GTACA BstEII 1329 GGTGACC G^GT[ACGT]ACC BstPI 1329 GGTGACC G^GT[ACGT]ACC CelII 1427 GCTGAGC GC^T[ACGT]AGC DraI 3364 TTTAAA TTT^AAA EcoO65I 1329 GGTGACC G^GT[ACGT]ACC FspI 2409 TGCGCA TGC^GCA HindIII 3350 AAGCTT A^AGCTT PshAI 359 GACTCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 2945 AACGTT AA^CGTT PvuI 1628 CGATCG CGAT^CG SacI 3153 GAGCTC GAGCT^C SapI 1188 GAAGAGC GCTCTTC(1/4) SmaI 807 CCCGGG CCC^GGG SmlI 2844 CTTAAG C^T[CT][GA]AG SspBI 2810 TGTACA T^GTACA StuI 2404 AGGCCT AGG^CCT Tsp509I 2006 AATT ^AATT XbaI 2822 TCTAGA T^CTAGA XmaCI 807 CCCGGG C^CCGGG XmaI 807 CCCGGG C^CCGGG XmnI 2942 GAAAACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AatII 18 GACGTC GACGT^C 2558 GACGTC AocI 1914 CCTGAGG CC^T[ACGT]AGG 3064 CCTCAGG AspI 1374 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1416 GACCGTGTC BbsI 3305 GAAGAC GAAGAC(2/6) 678 GTCTTC BglI 709 GCCCACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2296 GCCCCAATGGC BpuAI 3305 GAAGAC GAAGAC(2/6) 678 GTCTTC BsaWI 955 ACCGGA [AT]^CCGG[AT] 2647 ACCGGA BsgI 1179 CTGCAC GTGCAG(16/14) 399 GTGCAG BsiEI 980 CGACCG CG[GA][CT]^CG 1628 CGATCG BsmBI 1878 CGTCTC CGTCTC(1/5) 550 GAGACG BspDI 2055 ATCGAT AT^CGAT 2079 ATCGAT BsrBI 243 GAGCGG CCGCTC(-3/-3) 3099 GAGCGG BsrFI 183 ACCGGC [GA]^CCGG[CT] 1731 ACCGGC BstYI 472 GGATCT [GA]^GATC[CT] 2819 GGATCT Bsu36I 1914 CCTGAGG CC^T[ACGT]AGG 3064 CCTCAGG Cfr10I 183 ACCGGC [GA]^CCGG[CT] 1731 ACCGGC ClaI 2055 ATCGAT AT^CGAT 2079 ATCGAT DraIII 1401 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG 1674 CACATTGTG DrdI 519 GACGTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1371 GACGACCCTGTC Eco81I 1914 CCTGAGG CC^T[ACGT]AGG 3064 CCTCAGG HaeII 1543 GGCGCT [GA]GCGC^[CT] 2260 AGCGCC HincII 947 GTTGAC GT[CT]^[GA]AC 1535 GTTGAC HindII 947 GTTGAC GT[CT]^[GA]AC 1535 GTTGAC KspI 79 CCGCGG CCGC^GG 106 CCGCGG MflI 472 GGATCT [GA]^GATC[CT] 2819 GGATCT NcoI 686 CCATGG C^CATGG 2483 CCATGG PflFI 1374 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1416 GACCGTGTC SacII 79 CCGCGG CCGC^GG 106 CCGCGG Tth111I 1374 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1416 GACCGTGTC XhoII 472 GGATCT [GA]^GATC[CT] 2819 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### Acc65I AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI Asp718I AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I Van91I