##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AccIII AcsI AflIII AgeI AhdI
Aor51HI ApoI AscI AseI AsnI
AspEI BamHI BbeI BbrPI BclI
BglII BsaAI BsaBI BseAI BsiWI
BsmI BspEI BspHI BspLU11I BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI HpaI KasI MamI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
RcaI RsrII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 516 GTAGAC GT^[AC][GT]AC
AclI 2945 AACGTT AA^CGTT
AflII 2844 CTTAAG C^TTAAG
Alw44I 1398 GTGCAC G^TGCAC
ApaLI 1398 GTGCAC G^TGCAC
Asp700I 2942 GAAAACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2409 TGCGCA TGC^GCA
AvrII 2921 CCTAGG C^CTAGG
BbvCI 2457 CCTCAGC CCTCAGC(-5/-2)
BcgI 1988 GCAGCAATATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1967 GTATCC GTATCC(6/5)
BfrI 2844 CTTAAG C^TTAAG
BlnI 2921 CCTAGG C^CTAGG
BlpI 1427 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1427 GCTGAGC GC^T[ACGT]AGC
Bsp1407I 2810 TGTACA T^GTACA
BsrDI 845 CATTGC GCAATG(2/0)
BsrGI 2810 TGTACA T^GTACA
BstEII 1329 GGTGACC G^GT[ACGT]ACC
BstPI 1329 GGTGACC G^GT[ACGT]ACC
CelII 1427 GCTGAGC GC^T[ACGT]AGC
DraI 3364 TTTAAA TTT^AAA
EcoO65I 1329 GGTGACC G^GT[ACGT]ACC
FspI 2409 TGCGCA TGC^GCA
HindIII 3350 AAGCTT A^AGCTT
PshAI 359 GACTCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2945 AACGTT AA^CGTT
PvuI 1628 CGATCG CGAT^CG
SacI 3153 GAGCTC GAGCT^C
SapI 1188 GAAGAGC GCTCTTC(1/4)
SmaI 807 CCCGGG CCC^GGG
SmlI 2844 CTTAAG C^T[CT][GA]AG
SspBI 2810 TGTACA T^GTACA
StuI 2404 AGGCCT AGG^CCT
Tsp509I 2006 AATT ^AATT
XbaI 2822 TCTAGA T^CTAGA
XmaCI 807 CCCGGG C^CCGGG
XmaI 807 CCCGGG C^CCGGG
XmnI 2942 GAAAACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AatII 18 GACGTC GACGT^C
2558 GACGTC
AocI 1914 CCTGAGG CC^T[ACGT]AGG
3064 CCTCAGG
AspI 1374 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1416 GACCGTGTC
BbsI 3305 GAAGAC GAAGAC(2/6)
678 GTCTTC
BglI 709 GCCCACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2296 GCCCCAATGGC
BpuAI 3305 GAAGAC GAAGAC(2/6)
678 GTCTTC
BsaWI 955 ACCGGA [AT]^CCGG[AT]
2647 ACCGGA
BsgI 1179 CTGCAC GTGCAG(16/14)
399 GTGCAG
BsiEI 980 CGACCG CG[GA][CT]^CG
1628 CGATCG
BsmBI 1878 CGTCTC CGTCTC(1/5)
550 GAGACG
BspDI 2055 ATCGAT AT^CGAT
2079 ATCGAT
BsrBI 243 GAGCGG CCGCTC(-3/-3)
3099 GAGCGG
BsrFI 183 ACCGGC [GA]^CCGG[CT]
1731 ACCGGC
BstYI 472 GGATCT [GA]^GATC[CT]
2819 GGATCT
Bsu36I 1914 CCTGAGG CC^T[ACGT]AGG
3064 CCTCAGG
Cfr10I 183 ACCGGC [GA]^CCGG[CT]
1731 ACCGGC
ClaI 2055 ATCGAT AT^CGAT
2079 ATCGAT
DraIII 1401 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
1674 CACATTGTG
DrdI 519 GACGTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1371 GACGACCCTGTC
Eco81I 1914 CCTGAGG CC^T[ACGT]AGG
3064 CCTCAGG
HaeII 1543 GGCGCT [GA]GCGC^[CT]
2260 AGCGCC
HincII 947 GTTGAC GT[CT]^[GA]AC
1535 GTTGAC
HindII 947 GTTGAC GT[CT]^[GA]AC
1535 GTTGAC
KspI 79 CCGCGG CCGC^GG
106 CCGCGG
MflI 472 GGATCT [GA]^GATC[CT]
2819 GGATCT
NcoI 686 CCATGG C^CATGG
2483 CCATGG
PflFI 1374 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1416 GACCGTGTC
SacII 79 CCGCGG CCGC^GG
106 CCGCGG
Tth111I 1374 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1416 GACCGTGTC
XhoII 472 GGATCT [GA]^GATC[CT]
2819 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI Asp718I
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
Van91I