##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AclI AcyI AflIII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BclI BglII BlnI BsaAI BsaBI
BsaHI BsiWI BsmI BspDI BspLU11I
BsrFI BssHII BssSI Bst1107I BstBI
BstYI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoRI FbaI
FseI FspI HaeII HgaI Hin1I
HpaI KasI KspI MamI MfeI
MflI MluI MunI NaeI NarI
NdeI NgoMIV NheI NotI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI Tth111I XbaI
XhoII XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 1206 GGTACC G^GTACC
AccI 1339 GTCTAC GT^[AC][GT]AC
AccII 436 CGCG CG^CG
AcsI 1909 AAATTT [GA]^AATT[CT]
AhdI 2292 GACTGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 158 GGATC GGATC(4/5)
ApoI 1909 AAATTT [GA]^AATT[CT]
Asp718I 1206 GGTACC G^GTACC
AspEI 2292 GACTGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 746 TGGCCA TGG^CCA
BcgI 152 GCACCAGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1307 GTATCC GTATCC(6/5)
BglI 2526 GCCACTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 490 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 490 GCTCAGC GC^T[ACGT]AGC
BsiEI 1597 CGGTCG CG[GA][CT]^CG
BsmBI 1605 GAGACG CGTCTC(1/5)
Bsp1407I 1381 TGTACA T^GTACA
BspHI 228 TCATGA T^CATGA
BsrDI 2521 GCAATG GCAATG(2/0)
BsrGI 1381 TGTACA T^GTACA
BstAPI 2543 GCAAGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1244 GGTTACC G^GT[ACGT]ACC
BstPI 1244 GGTTACC G^GT[ACGT]ACC
BstUI 436 CGCG CG^CG
CelII 490 GCTCAGC GC^T[ACGT]AGC
CfoI 365 GCGC GCG^C
DsaI 2606 CCATGG C^C[GA][CT]GG
Eam1105I 2292 GACTGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2040 CCTGTGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1244 GGTTACC G^GT[ACGT]ACC
EcoT22I 1623 ATGCAT ATGCA^T
HhaI 365 GCGC GCG^C
HinP1I 365 GCGC G^CGC
HincII 2497 GTTGAC GT[CT]^[GA]AC
HindII 2497 GTTGAC GT[CT]^[GA]AC
KpnI 1206 GGTACC GGTAC^C
MluNI 746 TGGCCA TGG^CCA
MscI 746 TGGCCA TGG^CCA
MvnI 436 CGCG CG^CG
NcoI 2606 CCATGG C^CATGG
NruI 435 TCGCGA TCG^CGA
NsiI 1623 ATGCAT ATGCA^T
NspI 854 GCATGC [GA]CATG^[CT]
PaeR7I 940 CTCGAG C^TCGAG
RcaI 228 TCATGA T^CATGA
SexAI 1202 ACCTGGT A^CC[AT]GGT
SphI 854 GCATGC GCATG^C
SspBI 1381 TGTACA T^GTACA
SspI 3241 AATATT AAT^ATT
StuI 1835 AGGCCT AGG^CCT
TaqI 941 TCGA T^CGA
TthHB8I 941 TCGA T^CGA
XhoI 940 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccIII 45 TCCGGA T^CCGGA
524 TCCGGA
AflII 136 CTTAAG C^TTAAG
2441 CTTAAG
ApaI 182 GGGCCC GGGCC^C
2459 GGGCCC
BanI 1206 GGTACC G^G[CT][GA]CC
1390 GGCACC
BfrI 136 CTTAAG C^TTAAG
2441 CTTAAG
BsaI 2233 GAGACC GGTCTC(1/5)
2738 GGTCTC
BseAI 45 TCCGGA T^CCGGA
524 TCCGGA
BspEI 45 TCCGGA T^CCGGA
524 TCCGGA
BsrBI 23 GAGCGG CCGCTC(-3/-3)
1449 GAGCGG
HindIII 1521 AAGCTT A^AGCTT
3205 AAGCTT
MroI 45 TCCGGA T^CCGGA
524 TCCGGA
SapI 2015 GAAGAGC GCTCTTC(1/4)
3000 GCTCTTC
SmaI 120 CCCGGG CCC^GGG
2269 CCCGGG
XcmI 2116 CCACTGATTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2890 CCACCTACAATGTGG
XmaCI 120 CCCGGG C^CCGGG
2269 CCCGGG
XmaI 120 CCCGGG C^CCGGG
2269 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI Cac8I Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoO109I EcoRII EcoRV EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I