##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
AhdI Alw44I AocI ApaLI AscI
AseI AsnI Asp700I AspEI AviII
AvrII BbeI BbrPI BcgI BglII
BlnI BsaWI BseAI BsiWI BspEI
BspLU11I BsrFI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
Cfr10I CpoI DraI DrdI Eam1105I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KasI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI XbaI XcmI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 2235 GGTACC G^GTACC
AcsI 2493 AAATTC [GA]^AATT[CT]
AcyI 1937 GGCGTC G[GA]^CG[CT]C
AflII 2240 CTTAAG C^TTAAG
AflIII 2321 ACACGT A^C[GA][CT]GT
Aor51HI 2447 AGCGCT AGC^GCT
ApaI 1033 GGGCCC GGGCC^C
ApoI 2493 AAATTC [GA]^AATT[CT]
Asp718I 2235 GGTACC G^GTACC
AspI 149 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1915 CCCGGG C^[CT]CG[GA]G
BamHI 815 GGATCC G^GATCC
BclI 967 TGATCA T^GATCA
BfrI 2240 CTTAAG C^TTAAG
BsaBI 307 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1937 GGCGTC G[GA]^CG[CT]C
BsiEI 25 CGGCCG CG[GA][CT]^CG
BsmI 1950 GAATGC GAATGC(1/-1)
BsoBI 1915 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2544 TGTACA T^GTACA
BspDI 167 ATCGAT AT^CGAT
BsrBI 778 CCGCTC CCGCTC(-3/-3)
BsrGI 2544 TGTACA T^GTACA
BssHII 76 GCGCGC G^CGCGC
ClaI 167 ATCGAT AT^CGAT
DraIII 852 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 25 CGGCCG C^GGCCG
EclXI 25 CGGCCG C^GGCCG
Eco47III 2447 AGCGCT AGC^GCT
Eco52I 25 CGGCCG C^GGCCG
FbaI 967 TGATCA T^GATCA
Hin1I 1937 GGCGTC G[GA]^CG[CT]C
KpnI 2235 GGTACC GGTAC^C
KspI 47 CCGCGG CCGC^GG
MamI 307 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1686 CCATGG C^CATGG
PflFI 149 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1072 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 944 CAGCTG CAG^CTG
SacII 47 CCGCGG CCGC^GG
SapI 2168 GAAGAGC GCTCTTC(1/4)
SexAI 2367 ACCTGGT A^CC[AT]GGT
SmaI 1915 CCCGGG CCC^GGG
SspBI 2544 TGTACA T^GTACA
SspI 2101 AATATT AAT^ATT
StuI 1906 AGGCCT AGG^CCT
Tth111I 149 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1072 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1915 CCCGGG C^CCGGG
XmaI 1915 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BbsI 18 GAAGAC GAAGAC(2/6)
632 GAAGAC
BfaI 126 CTAG C^TAG
396 CTAG
BpuAI 18 GAAGAC GAAGAC(2/6)
632 GAAGAC
BsaAI 1062 TACGTG [CT]AC^GT[GA]
2322 CACGTA
BsaI 2460 GAGACC GGTCTC(1/5)
1492 GGTCTC
BsmBI 35 CGTCTC CGTCTC(1/5)
1939 CGTCTC
BspHI 867 TCATGA T^CATGA
1020 TCATGA
BsrDI 954 CATTGC GCAATG(2/0)
924 GCAATG
BstAPI 1384 GCAGCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2086 GCAGGTTTTGC
BstYI 815 GGATCC [GA]^GATC[CT]
2357 GGATCT
DsaI 47 CCGCGG C^C[GA][CT]GG
1686 CCATGG
EcoNI 553 CCTTGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1633 CCTGAGTGAGG
HphI 494 GGTGA GGTGA(8/7)
851 TCACC
MaeI 126 CTAG C^TAG
396 CTAG
MflI 815 GGATCC [GA]^GATC[CT]
2357 GGATCT
PpuMI 51 GGGTCCC [GA]G^G[AT]CC[CT]
978 AGGTCCT
RcaI 867 TCATGA T^CATGA
1020 TCATGA
SacI 497 GAGCTC GAGCT^C
839 GAGCTC
SphI 727 GCATGC GCATG^C
1158 GCATGC
XhoII 815 GGATCC [GA]^GATC[CT]
2357 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BalI BanI
BanII BbvCI BbvI BciVI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII ItaI Ksp632I
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I