##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AflIII AgeI ApoI AscI AseI
AsnI AvrII BbrPI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsiEI BsiWI BspDI
BspHI BspLU11I BspMI BsrDI BssHII
Bst1107I BstBI BstXI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco52I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI MamI MfeI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 1261 GGTACC G^GTACC
AccII 25 CGCG CG^CG
AccIII 2471 TCCGGA T^CCGGA
AhdI 2742 GACTGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 403 AGCGCT AGC^GCT
Asp700I 1792 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1261 GGTACC G^GTACC
AspEI 2742 GACTGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1533 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2352 TGCGCA TGC^GCA
BbeI 94 GGCGCC GGCGC^C
BcgI 2007 GCATCGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaWI 2471 TCCGGA [AT]^CCGG[AT]
BseAI 2471 TCCGGA T^CCGGA
BsmBI 1055 GAGACG CGTCTC(1/5)
BsmI 124 GCATTC GAATGC(1/-1)
Bsp1407I 2086 TGTACA T^GTACA
BspEI 2471 TCCGGA T^CCGGA
BsrFI 1504 ACCGGC [GA]^CCGG[CT]
BsrGI 2086 TGTACA T^GTACA
BssSI 102 CTCGTG CACGAG(-5/-1)
BstAPI 1870 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2131 GGTGACC G^GT[ACGT]ACC
BstPI 2131 GGTGACC G^GT[ACGT]ACC
BstUI 25 CGCG CG^CG
Cfr10I 1504 ACCGGC [GA]^CCGG[CT]
Eam1105I 2742 GACTGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 403 AGCGCT AGC^GCT
EcoO65I 2131 GGTGACC G^GT[ACGT]ACC
FspI 2352 TGCGCA TGC^GCA
KasI 94 GGCGCC G^GCGCC
KpnI 1261 GGTACC GGTAC^C
KspI 24 CCGCGG CCGC^GG
MaeII 2490 ACGT A^CGT
MroI 2471 TCCGGA T^CCGGA
MseI 2800 TTAA T^TAA
MvnI 25 CGCG CG^CG
NarI 94 GGCGCC GG^CGCC
PflFI 1533 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 24 CCGCGG CCGC^GG
SapI 1492 GAAGAGC GCTCTTC(1/4)
SfiI 2284 GGCCATCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 94 GGCGCC GGC^GCC
SmaI 1824 CCCGGG CCC^GGG
SspBI 2086 TGTACA T^GTACA
Tru9I 2800 TTAA T^TAA
Tsp509I 2039 AATT ^AATT
Tth111I 1533 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2286 CCATCAAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1824 CCCGGG C^CCGGG
XmaI 1824 CCCGGG C^CCGGG
XmnI 1792 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 94 GGCGCC G[GA]^CG[CT]C
1454 GGCGTC
Alw44I 1217 GTGCAC G^TGCAC
1782 GTGCAC
AocI 1418 CCTAAGG CC^T[ACGT]AGG
1523 CCTAAGG
ApaI 441 GGGCCC GGGCC^C
479 GGGCCC
ApaLI 1217 GTGCAC G^TGCAC
1782 GTGCAC
BalI 327 TGGCCA TGG^CCA
2683 TGGCCA
BamHI 69 GGATCC G^GATCC
1207 GGATCC
BfaI 531 CTAG C^TAG
875 CTAG
BglI 339 GCCCTCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2285 GCCATCAAGGC
BsaHI 94 GGCGCC G[GA]^CG[CT]C
1454 GGCGTC
BsrBI 2467 CCGCTC CCGCTC(-3/-3)
1922 GAGCGG
Bsu36I 1418 CCTAAGG CC^T[ACGT]AGG
1523 CCTAAGG
EaeI 327 TGGCCA [CT]^GGCC[GA]
2683 TGGCCA
EarI 1492 GAAGAG CTCTTC(1/4)
2597 GAAGAG
Eco81I 1418 CCTAAGG CC^T[ACGT]AGG
1523 CCTAAGG
EcoNI 1481 CCTAACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2274 CCTGGGCGAGG
HgaI 453 GACGC GACGC(5/10)
1455 GCGTC
Hin1I 94 GGCGCC G[GA]^CG[CT]C
1454 GGCGTC
Ksp632I 1492 GAAGAG CTCTTC(1/4)
2597 GAAGAG
MaeI 531 CTAG C^TAG
875 CTAG
MluNI 327 TGGCCA TGG^CCA
2683 TGGCCA
MscI 327 TGGCCA TGG^CCA
2683 TGGCCA
MslI 607 CATGATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1330 CATCCGAATG
PflMI 263 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
973 CCAGAAGATGG
SacI 1276 GAGCTC GAGCT^C
1495 GAGCTC
Van91I 263 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
973 CCAGAAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I XhoII