##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
Acc65I AccII AcsI AflII AgeI
AhdI Alw44I AocI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbeI
BbrPI BciVI BfrI BglII BlnI
BsaBI BsiWI BsmI Bsp1407I BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I Bsu36I DraI DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoRI
EcoRV FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MunI MvnI
NarI NdeI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI SacI SacII
SalI SapI SbfI ScaI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 1746 GACGTC GACGT^C
AccI 54 GTAGAC GT^[AC][GT]AC
AccIII 1848 TCCGGA T^CCGGA
AclI 103 AACGTT AA^CGTT
Aor51HI 922 AGCGCT AGC^GCT
AspHI 2505 GAGCAC G[AT]GC[AT]^C
BbvCI 2311 GCTGAGG CCTCAGC(-5/-2)
BcgI 1145 CGAATGGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1563 TGATCA T^GATCA
BsaAI 1319 TACGTG [CT]AC^GT[GA]
BsaI 1707 GAGACC GGTCTC(1/5)
BsaWI 1848 TCCGGA [AT]^CCGG[AT]
BseAI 1848 TCCGGA T^CCGGA
BsiEI 521 CGATCG CG[GA][CT]^CG
BsiHKAI 2505 GAGCAC G[AT]GC[AT]^C
BsmBI 1748 CGTCTC CGTCTC(1/5)
BspDI 519 ATCGAT AT^CGAT
BspEI 1848 TCCGGA T^CCGGA
BspHI 2284 TCATGA T^CATGA
BsrBI 1250 GAGCGG CCGCTC(-3/-3)
BstEII 2360 GGTCACC G^GT[ACGT]ACC
BstPI 2360 GGTCACC G^GT[ACGT]ACC
BstYI 414 GGATCT [GA]^GATC[CT]
ClaI 519 ATCGAT AT^CGAT
CpoI 1210 CGGACCG CG^G[AT]CCG
DraIII 253 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 922 AGCGCT AGC^GCT
EcoO65I 2360 GGTCACC G^GT[ACGT]ACC
EcoT14I 1075 CCATGG C^C[AT][AT]GG
EcoT22I 1153 ATGCAT ATGCA^T
FbaI 1563 TGATCA T^GATCA
MflI 414 GGATCT [GA]^GATC[CT]
MroI 1848 TCCGGA T^CCGGA
MseI 2501 TTAA T^TAA
NaeI 508 GCCGGC GCC^GGC
NcoI 1075 CCATGG C^CATGG
NgoMIV 508 GCCGGC G^CCGGC
NheI 677 GCTAGC G^CTAGC
NsiI 1153 ATGCAT ATGCA^T
PpuMI 2526 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 103 AACGTT AA^CGTT
PvuI 521 CGATCG CGAT^CG
RcaI 2284 TCATGA T^CATGA
RsrII 1210 CGGACCG CG^G[AT]CCG
SexAI 616 ACCTGGT A^CC[AT]GGT
SfiI 1627 GGCCCAACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 432 CTTGAG C^T[CT][GA]AG
StuI 1137 AGGCCT AGG^CCT
StyI 1075 CCATGG C^C[AT][AT]GG
Tru9I 2501 TTAA T^TAA
Tsp45I 2361 GTCAC ^GT[GC]AC
Tsp509I 2613 AATT ^AATT
XhoII 414 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 144 GACGCC G[GA]^CG[CT]C
1746 GACGTC
BalI 1269 TGGCCA TGG^CCA
1608 TGGCCA
BbsI 2429 GAAGAC GAAGAC(2/6)
2468 GTCTTC
BpuAI 2429 GAAGAC GAAGAC(2/6)
2468 GTCTTC
BsaHI 144 GACGCC G[GA]^CG[CT]C
1746 GACGTC
BsrDI 266 CATTGC GCAATG(2/0)
1493 GCAATG
BsrFI 508 GCCGGC [GA]^CCGG[CT]
1725 ACCGGC
Cfr10I 508 GCCGGC [GA]^CCGG[CT]
1725 ACCGGC
EarI 126 CTCTTC CTCTTC(1/4)
820 CTCTTC
HgaI 144 GACGC GACGC(5/10)
1525 GACGC
Hin1I 144 GACGCC G[GA]^CG[CT]C
1746 GACGTC
Ksp632I 126 CTCTTC CTCTTC(1/4)
820 CTCTTC
MaeIII 869 GTTAC ^GT[ACGT]AC
2361 GTCAC
MluNI 1269 TGGCCA TGG^CCA
1608 TGGCCA
MscI 1269 TGGCCA TGG^CCA
1608 TGGCCA
PflMI 752 CCACGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1465 CCACGCACTGG
PleI 1766 GAGTC GAGTC(4/5)
1943 GAGTC
TaqI 142 TCGA T^CGA
520 TCGA
TthHB8I 142 TCGA T^CGA
520 TCGA
Van91I 752 CCACGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1465 CCACGCACTGG
XcmI 1845 CCATCCGGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2544 CCAGCATCCACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI ApaI AspI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspLU11I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstXI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI TfiI TseI TspRI Tth111I
XmaCI XmaI