##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI Aor51HI AscI AseI AsnI
Asp718I AspI AviII AvrII BbrPI
BcgI BciVI BclI BglI BlnI
BsaAI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspMI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoT22I FbaI FseI FspI HpaI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcsI 1223 GAATTT [GA]^AATT[CT]
AflII 1896 CTTAAG C^TTAAG
AhdI 1754 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1247 GTGCAC G^TGCAC
ApaI 1357 GGGCCC GGGCC^C
ApaLI 1247 GTGCAC G^TGCAC
ApoI 1223 GAATTT [GA]^AATT[CT]
Asp700I 1386 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1754 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 62 GGATCC G^GATCC
BbeI 50 GGCGCC GGCGC^C
BfrI 1896 CTTAAG C^TTAAG
BglII 586 AGATCT A^GATCT
BlpI 177 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 177 GCTCAGC GC^T[ACGT]AGC
BsaBI 2114 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1944 GCATTC GAATGC(1/-1)
BspLU11I 44 ACATGT A^CATGT
BsrBI 166 CCGCTC CCGCTC(-3/-3)
BsrDI 1190 GCAATG GCAATG(2/0)
CelII 177 GCTCAGC GC^T[ACGT]AGC
Eam1105I 1754 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1970 GATATC GAT^ATC
HaeII 50 GGCGCC [GA]GCGC^[CT]
HgaI 295 GACGC GACGC(5/10)
HindIII 1109 AAGCTT A^AGCTT
KasI 50 GGCGCC G^GCGCC
MamI 2114 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 386 CAGCTG C[AC]G^C[GT]G
NarI 50 GGCGCC GG^CGCC
NheI 643 GCTAGC G^CTAGC
PaeR7I 907 CTCGAG C^TCGAG
PvuII 386 CAGCTG CAG^CTG
SacI 74 GAGCTC GAGCT^C
SalI 1783 GTCGAC G^TCGAC
SexAI 2001 ACCAGGT A^CC[AT]GGT
SfoI 50 GGCGCC GGC^GCC
SphI 639 GCATGC GCATG^C
StuI 1443 AGGCCT AGG^CCT
XhoI 907 CTCGAG C^TCGAG
XmnI 1386 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 50 GGCGCC G[GA]^CG[CT]C
295 GACGCC
AflIII 44 ACATGT A^C[GA][CT]GT
1709 ACGTGT
AocI 510 CCTCAGG CC^T[ACGT]AGG
1439 CCTCAGG
BbsI 535 GAAGAC GAAGAC(2/6)
1865 GAAGAC
BpuAI 535 GAAGAC GAAGAC(2/6)
1865 GAAGAC
BsaHI 50 GGCGCC G[GA]^CG[CT]C
295 GACGCC
BsaI 2253 GAGACC GGTCTC(1/5)
140 GGTCTC
Bsu36I 510 CCTCAGG CC^T[ACGT]AGG
1439 CCTCAGG
CfoI 51 GCGC GCG^C
1736 GCGC
Eco81I 510 CCTCAGG CC^T[ACGT]AGG
1439 CCTCAGG
HhaI 51 GCGC GCG^C
1736 GCGC
Hin1I 50 GGCGCC G[GA]^CG[CT]C
295 GACGCC
HinP1I 51 GCGC G^CGC
1736 GCGC
HincII 1762 GTCAAC GT[CT]^[GA]AC
1783 GTCGAC
HindII 1762 GTCAAC GT[CT]^[GA]AC
1783 GTCGAC
MaeII 1343 ACGT A^CGT
1709 ACGT
MslI 554 CATCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1144 CACCTGGATG
NcoI 1512 CCATGG C^CATGG
1889 CCATGG
PshAI 1382 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2150 GACTTCAGTC
SmaI 1316 CCCGGG CCC^GGG
1722 CCCGGG
Tsp509I 1224 AATT ^AATT
1625 AATT
XcmI 1107 CCAAGCTTTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1904 CCACCAGGCTGCTGG
XmaCI 1316 CCCGGG C^CCGGG
1722 CCCGGG
XmaI 1316 CCCGGG C^CCGGG
1722 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
TspRI TthHB8I Van91I XhoII