################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BalI BamHI BbeI BbvCI BcgI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BssHII Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoRI EcoT22I FspI Hin1I HpaI KasI KpnI KspI MamI MluI MluNI MroI MscI MvnI NarI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AlwNI 2043 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 1941 AGCGCT AGC^GCT Asp700I 688 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 224 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 648 CCCGAG C^[CT]CG[GA]G BanI 293 GGCACC G^G[CT][GA]CC BbrPI 1808 CACGTG CAC^GTG BciVI 2341 GTATCC GTATCC(6/5) BcnI 267 CCGGG CC^[GC]GG BglI 50 GCCAGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 1808 CACGTG [CT]AC^GT[GA] BsaI 1466 GAGACC GGTCTC(1/5) BsgI 714 GTGCAG GTGCAG(16/14) BsoBI 648 CCCGAG C^[CT]CG[GA]G Bsp1407I 1252 TGTACA T^GTACA BspMI 366 ACCTGC ACCTGC(4/8) BsrGI 1252 TGTACA T^GTACA BssSI 459 CTCGTG CACGAG(-5/-1) BstEII 2094 GGTCACC G^GT[ACGT]ACC BstPI 2094 GGTCACC G^GT[ACGT]ACC EaeI 991 CGGCCA [CT]^GGCC[GA] Eco47III 1941 AGCGCT AGC^GCT EcoO65I 2094 GGTCACC G^GT[ACGT]ACC EcoRV 262 GATATC GAT^ATC FseI 54 GGCCGGCC GGCCGG^CC HaeII 1941 AGCGCT [GA]GCGC^[CT] HindIII 1795 AAGCTT A^AGCTT MfeI 79 CAATTG C^AATTG MspA1I 1166 CAGCGG C[AC]G^C[GT]G MunI 79 CAATTG C^AATTG NciI 267 CCGGG CC^[GC]GG NdeI 282 CATATG CA^TATG PflFI 224 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1808 CACGTG CAC^GTG PmlI 1808 CACGTG CAC^GTG SfiI 49 GGCCAGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspBI 1252 TGTACA T^GTACA TaqI 603 TCGA T^CGA Tth111I 224 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 603 TCGA T^CGA XmnI 688 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 672 AAATTC [GA]^AATT[CT] 1091 AAATTC ApaI 1987 GGGCCC GGGCC^C 2069 GGGCCC ApoI 672 AAATTC [GA]^AATT[CT] 1091 AAATTC BbsI 33 GAAGAC GAAGAC(2/6) 1502 GTCTTC BclI 589 TGATCA T^GATCA 1804 TGATCA BpuAI 33 GAAGAC GAAGAC(2/6) 1502 GTCTTC BspHI 247 TCATGA T^CATGA 1629 TCATGA BsrFI 55 GCCGGC [GA]^CCGG[CT] 2020 GCCGGC CfoI 1546 GCGC GCG^C 1942 GCGC Cfr10I 55 GCCGGC [GA]^CCGG[CT] 2020 GCCGGC FbaI 589 TGATCA T^GATCA 1804 TGATCA HgaI 816 GACGC GACGC(5/10) 228 GCGTC HhaI 1546 GCGC GCG^C 1942 GCGC HinP1I 1546 GCGC G^CGC 1942 GCGC NaeI 55 GCCGGC GCC^GGC 2020 GCCGGC NgoMIV 55 GCCGGC G^CCGGC 2020 GCCGGC PpuMI 269 GGGACCC [GA]G^G[AT]CC[CT] 2182 GGGACCC PstI 596 CTGCAG CTGCA^G 1388 CTGCAG RcaI 247 TCATGA T^CATGA 1629 TCATGA SfcI 596 CTGCAG C^T[GA][CT]AG 1388 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaII BanII BbvI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrBI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NcoI NdeII NlaIII NlaIV PflMI PleI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII