##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BalI BamHI BbeI BbvCI
BcgI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspLU11I BssHII Bst1107I BstAPI
BstBI BstUI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoRI EcoT22I FspI Hin1I
HpaI KasI KpnI KspI MamI
MluI MluNI MroI MscI MvnI
NarI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AlwNI 2043 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1941 AGCGCT AGC^GCT
Asp700I 688 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 224 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 648 CCCGAG C^[CT]CG[GA]G
BanI 293 GGCACC G^G[CT][GA]CC
BbrPI 1808 CACGTG CAC^GTG
BciVI 2341 GTATCC GTATCC(6/5)
BcnI 267 CCGGG CC^[GC]GG
BglI 50 GCCAGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1808 CACGTG [CT]AC^GT[GA]
BsaI 1466 GAGACC GGTCTC(1/5)
BsgI 714 GTGCAG GTGCAG(16/14)
BsoBI 648 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1252 TGTACA T^GTACA
BspMI 366 ACCTGC ACCTGC(4/8)
BsrGI 1252 TGTACA T^GTACA
BssSI 459 CTCGTG CACGAG(-5/-1)
BstEII 2094 GGTCACC G^GT[ACGT]ACC
BstPI 2094 GGTCACC G^GT[ACGT]ACC
EaeI 991 CGGCCA [CT]^GGCC[GA]
Eco47III 1941 AGCGCT AGC^GCT
EcoO65I 2094 GGTCACC G^GT[ACGT]ACC
EcoRV 262 GATATC GAT^ATC
FseI 54 GGCCGGCC GGCCGG^CC
HaeII 1941 AGCGCT [GA]GCGC^[CT]
HindIII 1795 AAGCTT A^AGCTT
MfeI 79 CAATTG C^AATTG
MspA1I 1166 CAGCGG C[AC]G^C[GT]G
MunI 79 CAATTG C^AATTG
NciI 267 CCGGG CC^[GC]GG
NdeI 282 CATATG CA^TATG
PflFI 224 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1808 CACGTG CAC^GTG
PmlI 1808 CACGTG CAC^GTG
SfiI 49 GGCCAGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 1252 TGTACA T^GTACA
TaqI 603 TCGA T^CGA
Tth111I 224 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 603 TCGA T^CGA
XmnI 688 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 672 AAATTC [GA]^AATT[CT]
1091 AAATTC
ApaI 1987 GGGCCC GGGCC^C
2069 GGGCCC
ApoI 672 AAATTC [GA]^AATT[CT]
1091 AAATTC
BbsI 33 GAAGAC GAAGAC(2/6)
1502 GTCTTC
BclI 589 TGATCA T^GATCA
1804 TGATCA
BpuAI 33 GAAGAC GAAGAC(2/6)
1502 GTCTTC
BspHI 247 TCATGA T^CATGA
1629 TCATGA
BsrFI 55 GCCGGC [GA]^CCGG[CT]
2020 GCCGGC
CfoI 1546 GCGC GCG^C
1942 GCGC
Cfr10I 55 GCCGGC [GA]^CCGG[CT]
2020 GCCGGC
FbaI 589 TGATCA T^GATCA
1804 TGATCA
HgaI 816 GACGC GACGC(5/10)
228 GCGTC
HhaI 1546 GCGC GCG^C
1942 GCGC
HinP1I 1546 GCGC G^CGC
1942 GCGC
NaeI 55 GCCGGC GCC^GGC
2020 GCCGGC
NgoMIV 55 GCCGGC G^CCGGC
2020 GCCGGC
PpuMI 269 GGGACCC [GA]G^G[AT]CC[CT]
2182 GGGACCC
PstI 596 CTGCAG CTGCA^G
1388 CTGCAG
RcaI 247 TCATGA T^CATGA
1629 TCATGA
SfcI 596 CTGCAG C^T[GA][CT]AG
1388 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbvI BfaI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrBI
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
PflMI PleI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI XhoII