##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI Aor51HI AscI AseI AsnI
Asp718I AspI AviII BamHI BanI
BbeI BbsI BbvCI BcgI BciVI
BpuAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI ClaI CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HaeII HincII HindII HpaI
KasI KpnI KspI MamI MluI
MroI MvnI NaeI NarI NgoMIV
NotI NruI NsiI NspI NspV
PacI PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 518 GTATAC GT^[AC][GT]AC
AcyI 1067 GACGCC G[GA]^CG[CT]C
AflII 1830 CTTAAG C^TTAAG
AflIII 774 ACGTGT A^C[GA][CT]GT
AhdI 1150 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 2279 GGATC GGATC(4/5)
AocI 2557 CCTTAGG CC^T[ACGT]AGG
ApaI 2085 GGGCCC GGGCC^C
Asp700I 2162 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1150 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BfrI 1830 CTTAAG C^TTAAG
BglII 1256 AGATCT A^GATCT
BlpI 2121 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2121 GCTCAGC GC^T[ACGT]AGC
BsaHI 1067 GACGCC G[GA]^CG[CT]C
BspHI 1508 TCATGA T^CATGA
BspMI 2260 ACCTGC ACCTGC(4/8)
Bst1107I 518 GTATAC GTA^TAC
BstYI 1256 AGATCT [GA]^GATC[CT]
BstZ17I 518 GTATAC GTA^TAC
Bsu36I 2557 CCTTAGG CC^T[ACGT]AGG
CelII 2121 GCTCAGC GC^T[ACGT]AGC
CfoI 1330 GCGC GCG^C
Eam1105I 1150 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2369 GAAGAG CTCTTC(1/4)
Eco81I 2557 CCTTAGG CC^T[ACGT]AGG
EcoNI 1625 CCTTGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HhaI 1330 GCGC GCG^C
Hin1I 1067 GACGCC G[GA]^CG[CT]C
HinP1I 1330 GCGC G^CGC
HindIII 1099 AAGCTT A^AGCTT
Ksp632I 2369 GAAGAG CTCTTC(1/4)
MfeI 388 CAATTG C^AATTG
MflI 1256 AGATCT [GA]^GATC[CT]
MunI 388 CAATTG C^AATTG
NdeI 1378 CATATG CA^TATG
NheI 568 GCTAGC G^CTAGC
PaeR7I 799 CTCGAG C^TCGAG
PflMI 1352 CCATGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2257 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 1508 TCATGA T^CATGA
SfaNI 769 GATGC GCATC(5/9)
SmaI 750 CCCGGG CCC^GGG
Van91I 1352 CCATGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 799 CTCGAG C^TCGAG
XhoII 1256 AGATCT [GA]^GATC[CT]
XmaCI 750 CCCGGG C^CCGGG
XmaI 750 CCCGGG C^CCGGG
XmnI 2162 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 1044 AAATTT [GA]^AATT[CT]
2689 GAATTT
Alw44I 892 GTGCAC G^TGCAC
1787 GTGCAC
AlwNI 1466 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
2448 CAGGGGCTG
ApaLI 892 GTGCAC G^TGCAC
1787 GTGCAC
ApoI 1044 AAATTT [GA]^AATT[CT]
2689 GAATTT
AvrII 1557 CCTAGG C^CTAGG
2504 CCTAGG
BbrPI 468 CACGTG CAC^GTG
773 CACGTG
BclI 167 TGATCA T^GATCA
862 TGATCA
BcnI 750 CCCGG CC^[GC]GG
751 CCGGG
BglI 1171 GCCCAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1366 GCCAGTGAGGC
BlnI 1557 CCTAGG C^CTAGG
2504 CCTAGG
BsaAI 468 CACGTG [CT]AC^GT[GA]
773 CACGTG
BsgI 2262 CTGCAC GTGCAG(16/14)
1755 GTGCAG
BsmI 1362 GAATGC GAATGC(1/-1)
1925 GAATGC
BstXI 336 CCATGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2173 CCAGAGCCCTGG
FauI 649 CCCGC CCCGC(4/6)
930 GCGGG
FbaI 167 TGATCA T^GATCA
862 TGATCA
HgaI 1067 GACGC GACGC(5/10)
2444 GACGC
MslI 337 CATGGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2678 CATTGAGGTG
MspA1I 1138 CAGCTG C[AC]G^C[GT]G
1427 CAGCTG
NciI 750 CCCGG CC^[GC]GG
751 CCGGG
PmaCI 468 CACGTG CAC^GTG
773 CACGTG
PmlI 468 CACGTG CAC^GTG
773 CACGTG
PstI 2114 CTGCAG CTGCA^G
2652 CTGCAG
PvuII 1138 CAGCTG CAG^CTG
1427 CAGCTG
SacI 536 GAGCTC GAGCT^C
2119 GAGCTC
SmlI 799 CTCGAG C^T[CT][GA]AG
1830 CTTAAG
SpeI 426 ACTAGT A^CTAGT
2136 ACTAGT
TaqI 665 TCGA T^CGA
800 TCGA
TthHB8I 665 TCGA T^CGA
800 TCGA
XcmI 1343 CCAAAGTGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2614 CCATGAGGGGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaI
AvaII BalI BanII BbvI BfaI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI Cac8I Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfcI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI