##################################
# NO CUT ENZYMES ( 42 enzymes) #
##################################
AatII Acc65I AclI AflII AseI
AsnI Asp718I AspI AviII AvrII
BciVI BfrI BlnI BsaBI Bst1107I
BstBI BstZ17I DrdI EcoRI EcoRV
FspI KpnI MamI NotI NruI
NspV PacI PaeR7I PflFI PmeI
PshBI Psp1406I SalI SbfI SexAI
SfuI Sse8387I SspI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 5606 TCCGGA T^CCGGA
AgeI 10 ACCGGT A^CCGGT
Aor51HI 2318 AGCGCT AGC^GCT
ApaI 3870 GGGCCC GGGCC^C
AscI 641 GGCGCGCC GG^CGCGCC
Asp700I 2950 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 1653 CACGTG CAC^GTG
BseAI 5606 TCCGGA T^CCGGA
Bsp1407I 4002 TGTACA T^GTACA
BspEI 5606 TCCGGA T^CCGGA
BspHI 1306 TCATGA T^CATGA
BspLU11I 4564 ACATGT A^CATGT
BsrDI 3486 GCAATG GCAATG(2/0)
BsrGI 4002 TGTACA T^GTACA
CpoI 140 CGGACCG CG^G[AT]CCG
DraIII 3285 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 676 CGGCCG C^GGCCG
EclXI 676 CGGCCG C^GGCCG
Eco47III 2318 AGCGCT AGC^GCT
Eco52I 676 CGGCCG C^GGCCG
FseI 428 GGCCGGCC GGCCGG^CC
HpaI 1853 GTTAAC GTT^AAC
MfeI 3472 CAATTG C^AATTG
MluI 152 ACGCGT A^CGCGT
MroI 5606 TCCGGA T^CCGGA
MunI 3472 CAATTG C^AATTG
NcoI 4398 CCATGG C^CATGG
PinAI 10 ACCGGT A^CCGGT
PmaCI 1653 CACGTG CAC^GTG
PmlI 1653 CACGTG CAC^GTG
PshAI 5135 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1378 CGATCG CGAT^CG
RcaI 1306 TCATGA T^CATGA
RsrII 140 CGGACCG CG^G[AT]CCG
ScaI 4764 AGTACT AGT^ACT
SfiI 2922 GGCCAGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 3447 TACGTA TAC^GTA
SspBI 4002 TGTACA T^GTACA
XmnI 2950 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AflIII 152 ACGCGT A^C[GA][CT]GT
4564 ACATGT
AhdI 966 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2362 GACTGGATGTC
AocI 359 CCTGAGG CC^T[ACGT]AGG
2259 CCTAAGG
AspEI 966 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2362 GACTGGATGTC
BalI 2174 TGGCCA TGG^CCA
2921 TGGCCA
BamHI 836 GGATCC G^GATCC
2253 GGATCC
BcgI 4669 CGAGTCACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5124 GCAGTTGGCTCG
BclI 3258 TGATCA T^GATCA
5619 TGATCA
BglII 4946 AGATCT A^GATCT
4991 AGATCT
BsaWI 10 ACCGGT [AT]^CCGG[AT]
5606 TCCGGA
BsiEI 676 CGGCCG CG[GA][CT]^CG
1378 CGATCG
BsiWI 686 CGTACG C^GTACG
1237 CGTACG
BsmBI 412 GAGACG CGTCTC(1/5)
5179 GAGACG
BsmI 2622 GAATGC GAATGC(1/-1)
4275 GCATTC
BspDI 1218 ATCGAT AT^CGAT
2348 ATCGAT
BsrBI 2685 GAGCGG CCGCTC(-3/-3)
4696 GAGCGG
BstAPI 1544 GCAGGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4245 GCAGTAACTGC
BstEII 4976 GGTGACC G^GT[ACGT]ACC
5525 GGTGACC
BstPI 4976 GGTGACC G^GT[ACGT]ACC
5525 GGTGACC
Bsu36I 359 CCTGAGG CC^T[ACGT]AGG
2259 CCTAAGG
ClaI 1218 ATCGAT AT^CGAT
2348 ATCGAT
Eam1105I 966 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2362 GACTGGATGTC
Eco81I 359 CCTGAGG CC^T[ACGT]AGG
2259 CCTAAGG
EcoO65I 4976 GGTGACC G^GT[ACGT]ACC
5525 GGTGACC
FbaI 3258 TGATCA T^GATCA
5619 TGATCA
HindIII 3809 AAGCTT A^AGCTT
5688 AAGCTT
KspI 400 CCGCGG CCGC^GG
914 CCGCGG
MluNI 2174 TGGCCA TGG^CCA
2921 TGGCCA
MscI 2174 TGGCCA TGG^CCA
2921 TGGCCA
NdeI 3978 CATATG CA^TATG
5652 CATATG
NheI 23 GCTAGC G^CTAGC
1052 GCTAGC
PflMI 620 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2659 CCAGATCCTGG
SacII 400 CCGCGG CCGC^GG
914 CCGCGG
SapI 3180 GAAGAGC GCTCTTC(1/4)
704 GCTCTTC
SgrAI 499 CGCCGGCG C[GA]^CCGG[CT]G
578 CGCCGGCG
SpeI 3316 ACTAGT A^CTAGT
4110 ACTAGT
StuI 2930 AGGCCT AGG^CCT
3820 AGGCCT
Van91I 620 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2659 CCAGATCCTGG
XcmI 2177 CCAGGCCCACGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3203 CCATCTACGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
ApaLI ApoI AspHI AvaI AvaII
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssHII BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV NsiI
NspI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
SphI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmaCI XmaI