################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AatII Acc65I AccII AclI AflII AgeI AscI Asp718I BbeI BbrPI BciVI BfrI BsaBI BsiEI BsiWI BsmI BspDI BsrFI BssHII Bst1107I BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI EagI EclXI Eco52I EcoNI EcoO65I EcoRV FseI HindIII KasI KpnI KspI MamI MfeI MluI MunI MvnI NaeI NarI NgoMIV NotI NruI PacI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccIII 295 TCCGGA T^CCGGA AcyI 229 GGCGTC G[GA]^CG[CT]C AflIII 3556 ACATGT A^C[GA][CT]GT AhdI 4531 GACTGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 4301 AGCGCT AGC^GCT AseI 5426 ATTAAT AT^TAAT AsnI 5426 ATTAAT AT^TAAT AspEI 4531 GACTGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2402 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 655 TGCGCA TGC^GCA AvrII 2528 CCTAGG C^CTAGG BanI 2912 GGTGCC G^G[CT][GA]CC BbvCI 3680 CCTCAGC CCTCAGC(-5/-2) BcgI 3650 CGATTGGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1638 TGATCA T^GATCA BglI 1870 GCCTCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 2528 CCTAGG C^CTAGG BlpI 4993 GCTCAGC GC^T[ACGT]AGC Bpu1102I 4993 GCTCAGC GC^T[ACGT]AGC BsaAI 3173 TACGTG [CT]AC^GT[GA] BsaHI 229 GGCGTC G[GA]^CG[CT]C BseAI 295 TCCGGA T^CCGGA BsmBI 1756 GAGACG CGTCTC(1/5) Bsp1407I 1098 TGTACA T^GTACA BspEI 295 TCCGGA T^CCGGA BspHI 1578 TCATGA T^CATGA BspLU11I 3556 ACATGT A^CATGT BsrDI 403 CATTGC GCAATG(2/0) BsrGI 1098 TGTACA T^GTACA BssSI 1342 CACGAG CACGAG(-5/-1) CelII 4993 GCTCAGC GC^T[ACGT]AGC Eam1105I 4531 GACTGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 4301 AGCGCT AGC^GCT FbaI 1638 TGATCA T^GATCA FspI 655 TGCGCA TGC^GCA Hin1I 229 GGCGTC G[GA]^CG[CT]C HpaI 2275 GTTAAC GTT^AAC MroI 295 TCCGGA T^CCGGA NdeI 659 CATATG CA^TATG NheI 3822 GCTAGC G^CTAGC PaeR7I 522 CTCGAG C^TCGAG PflFI 2402 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 1437 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 5426 ATTAAT AT^TAAT RcaI 1578 TCATGA T^CATGA SacI 1076 GAGCTC GAGCT^C SapI 439 GCTCTTC GCTCTTC(1/4) SexAI 1745 ACCTGGT A^CC[AT]GGT SspBI 1098 TGTACA T^GTACA SspI 2391 AATATT AAT^ATT Tth111I 2402 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 757 CCAGATGCCCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 522 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AocI 200 CCTCAGG CC^T[ACGT]AGG 4697 CCTCAGG ApaI 3413 GGGCCC GGGCC^C 4702 GGGCCC BamHI 1409 GGATCC G^GATCC 4935 GGATCC BsaWI 89 ACCGGA [AT]^CCGG[AT] 295 TCCGGA BstAPI 17 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 109 GCAGGAGGTGC BstBI 615 TTCGAA TT^CGAA 2036 TTCGAA Bsu36I 200 CCTCAGG CC^T[ACGT]AGG 4697 CCTCAGG DraIII 845 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG 3342 CACTCAGTG DrdI 2153 GACTGGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4535 GACAGTCAGGTC Eco81I 200 CCTCAGG CC^T[ACGT]AGG 4697 CCTCAGG EcoRI 1789 GAATTC G^AATTC 5414 GAATTC HaeII 208 GGCGCT [GA]GCGC^[CT] 4301 AGCGCT HgaI 1758 GACGC GACGC(5/10) 230 GCGTC NcoI 490 CCATGG C^CATGG 594 CCATGG NspV 615 TTCGAA TT^CGAA 2036 TTCGAA SalI 2224 GTCGAC G^TCGAC 2408 GTCGAC SfuI 615 TTCGAA TT^CGAA 2036 TTCGAA SphI 1352 GCATGC GCATG^C 3577 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccI AciI AcsI AfaI AluI Alw44I AlwI AlwNI ApaLI ApoI Asp700I AspHI AvaI AvaII BalI BanII BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI