##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AgeI AscI Asp718I BbeI BbrPI
BciVI BfrI BsaBI BsiEI BsiWI
BsmI BspDI BsrFI BssHII Bst1107I
BstEII BstPI BstUI BstZ17I Cfr10I
ClaI CpoI EagI EclXI Eco52I
EcoNI EcoO65I EcoRV FseI HindIII
KasI KpnI KspI MamI MfeI
MluI MunI MvnI NaeI NarI
NgoMIV NotI NruI PacI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SbfI ScaI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccIII 295 TCCGGA T^CCGGA
AcyI 229 GGCGTC G[GA]^CG[CT]C
AflIII 3556 ACATGT A^C[GA][CT]GT
AhdI 4531 GACTGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4301 AGCGCT AGC^GCT
AseI 5426 ATTAAT AT^TAAT
AsnI 5426 ATTAAT AT^TAAT
AspEI 4531 GACTGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2402 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 655 TGCGCA TGC^GCA
AvrII 2528 CCTAGG C^CTAGG
BanI 2912 GGTGCC G^G[CT][GA]CC
BbvCI 3680 CCTCAGC CCTCAGC(-5/-2)
BcgI 3650 CGATTGGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1638 TGATCA T^GATCA
BglI 1870 GCCTCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2528 CCTAGG C^CTAGG
BlpI 4993 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4993 GCTCAGC GC^T[ACGT]AGC
BsaAI 3173 TACGTG [CT]AC^GT[GA]
BsaHI 229 GGCGTC G[GA]^CG[CT]C
BseAI 295 TCCGGA T^CCGGA
BsmBI 1756 GAGACG CGTCTC(1/5)
Bsp1407I 1098 TGTACA T^GTACA
BspEI 295 TCCGGA T^CCGGA
BspHI 1578 TCATGA T^CATGA
BspLU11I 3556 ACATGT A^CATGT
BsrDI 403 CATTGC GCAATG(2/0)
BsrGI 1098 TGTACA T^GTACA
BssSI 1342 CACGAG CACGAG(-5/-1)
CelII 4993 GCTCAGC GC^T[ACGT]AGC
Eam1105I 4531 GACTGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4301 AGCGCT AGC^GCT
FbaI 1638 TGATCA T^GATCA
FspI 655 TGCGCA TGC^GCA
Hin1I 229 GGCGTC G[GA]^CG[CT]C
HpaI 2275 GTTAAC GTT^AAC
MroI 295 TCCGGA T^CCGGA
NdeI 659 CATATG CA^TATG
NheI 3822 GCTAGC G^CTAGC
PaeR7I 522 CTCGAG C^TCGAG
PflFI 2402 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1437 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5426 ATTAAT AT^TAAT
RcaI 1578 TCATGA T^CATGA
SacI 1076 GAGCTC GAGCT^C
SapI 439 GCTCTTC GCTCTTC(1/4)
SexAI 1745 ACCTGGT A^CC[AT]GGT
SspBI 1098 TGTACA T^GTACA
SspI 2391 AATATT AAT^ATT
Tth111I 2402 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 757 CCAGATGCCCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 522 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AocI 200 CCTCAGG CC^T[ACGT]AGG
4697 CCTCAGG
ApaI 3413 GGGCCC GGGCC^C
4702 GGGCCC
BamHI 1409 GGATCC G^GATCC
4935 GGATCC
BsaWI 89 ACCGGA [AT]^CCGG[AT]
295 TCCGGA
BstAPI 17 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
109 GCAGGAGGTGC
BstBI 615 TTCGAA TT^CGAA
2036 TTCGAA
Bsu36I 200 CCTCAGG CC^T[ACGT]AGG
4697 CCTCAGG
DraIII 845 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
3342 CACTCAGTG
DrdI 2153 GACTGGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4535 GACAGTCAGGTC
Eco81I 200 CCTCAGG CC^T[ACGT]AGG
4697 CCTCAGG
EcoRI 1789 GAATTC G^AATTC
5414 GAATTC
HaeII 208 GGCGCT [GA]GCGC^[CT]
4301 AGCGCT
HgaI 1758 GACGC GACGC(5/10)
230 GCGTC
NcoI 490 CCATGG C^CATGG
594 CCATGG
NspV 615 TTCGAA TT^CGAA
2036 TTCGAA
SalI 2224 GTCGAC G^TCGAC
2408 GTCGAC
SfuI 615 TTCGAA TT^CGAA
2036 TTCGAA
SphI 1352 GCATGC GCATG^C
3577 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
Asp700I AspHI AvaI AvaII BalI
BanII BbsI BbvI BcnI BfaI
BglII BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI