##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AccII AccIII AclI AflII AgeI
Aor51HI AscI Asp700I AviII BbeI
BcgI BfrI BsaBI BsaWI BseAI
BsiWI Bsp1407I BspDI BspEI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CfoI ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoO65I EcoRV FseI FspI HaeII
HhaI HinP1I HincII HindII HpaI
KasI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmeI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 5634 GACGTC GACGT^C
AseI 174 ATTAAT AT^TAAT
AsnI 174 ATTAAT AT^TAAT
AspI 4753 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1516 GGATCC G^GATCC
BbsI 4759 GTCTTC GAAGAC(2/6)
BpuAI 4759 GTCTTC GAAGAC(2/6)
BsiEI 2868 CGGTCG CG[GA][CT]^CG
BspHI 4415 TCATGA T^CATGA
BspLU11I 2316 ACATGT A^CATGT
BsrBI 999 GAGCGG CCGCTC(-3/-3)
BsrFI 2866 GCCGGT [GA]^CCGG[CT]
BssSI 983 CACGAG CACGAG(-5/-1)
Cfr10I 2866 GCCGGT [GA]^CCGG[CT]
DraIII 3926 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 693 GACGCCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3515 GAATTC G^AATTC
HgaI 693 GACGC GACGC(5/10)
HindIII 1245 AAGCTT A^AGCTT
NdeI 3251 CATATG CA^TATG
PaeR7I 1457 CTCGAG C^TCGAG
PflFI 4753 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 174 ATTAAT AT^TAAT
RcaI 4415 TCATGA T^CATGA
SapI 1022 GCTCTTC GCTCTTC(1/4)
SexAI 4754 ACCTGGT A^CC[AT]GGT
SfiI 3214 GGCCCACGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 3954 CCCGGG CCC^GGG
SphI 2545 GCATGC GCATG^C
StuI 5530 AGGCCT AGG^CCT
Tth111I 4753 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1457 CTCGAG C^TCGAG
XmaCI 3954 CCCGGG C^CCGGG
XmaI 3954 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 1281 GGTACC G^GTACC
5589 GGTACC
AccI 338 GTCTAC GT^[AC][GT]AC
965 GTAGAC
AcyI 693 GACGCC G[GA]^CG[CT]C
5634 GACGTC
Alw44I 1100 GTGCAC G^TGCAC
2320 GTGCAC
ApaI 3222 GGGCCC GGGCC^C
3897 GGGCCC
ApaLI 1100 GTGCAC G^TGCAC
2320 GTGCAC
Asp718I 1281 GGTACC G^GTACC
5589 GGTACC
AvrII 3350 CCTAGG C^CTAGG
4562 CCTAGG
BbvCI 3736 CCTCAGC CCTCAGC(-5/-2)
4702 GCTGAGG
BclI 3711 TGATCA T^GATCA
4665 TGATCA
BglI 3215 GCCCACGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3618 GCCTGGAAGGC
BlnI 3350 CCTAGG C^CTAGG
4562 CCTAGG
BlpI 1839 GCTCAGC GC^T[ACGT]AGC
5181 GCTCAGC
Bpu1102I 1839 GCTCAGC GC^T[ACGT]AGC
5181 GCTCAGC
BsaHI 693 GACGCC G[GA]^CG[CT]C
5634 GACGTC
BsmBI 5636 CGTCTC CGTCTC(1/5)
1672 GAGACG
BsmI 3308 GAATGC GAATGC(1/-1)
5264 GAATGC
BsrDI 3312 GCAATG GCAATG(2/0)
3469 GCAATG
CelII 1839 GCTCAGC GC^T[ACGT]AGC
5181 GCTCAGC
EcoNI 4126 CCTCAGATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5141 CCTTACCCAGG
EcoT22I 2300 ATGCAT ATGCA^T
3800 ATGCAT
FbaI 3711 TGATCA T^GATCA
4665 TGATCA
Hin1I 693 GACGCC G[GA]^CG[CT]C
5634 GACGTC
KpnI 1281 GGTACC GGTAC^C
5589 GGTACC
NsiI 2300 ATGCAT ATGCA^T
3800 ATGCAT
ScaI 588 AGTACT AGT^ACT
2974 AGTACT
XbaI 3304 TCTAGA T^CTAGA
5355 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AfaI AflIII AhdI
AluI AlwI AlwNI AocI ApoI
AspEI AspHI AvaI AvaII BalI
BanI BanII BbrPI BbvI BciVI
BcnI BfaI BglII BmyI BpmI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI Eam1105I EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII