##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspI AviII
BbeI BbvCI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BsrBI
BssHII BstBI BstEII BstPI BstUI
ClaI CpoI EagI EclXI Eco47III
Eco52I EcoO65I FseI FspI HaeII
HindIII HpaI KasI KpnI KspI
MluI MroI MvnI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AhdI 1189 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 446 CCTGAGG CC^T[ACGT]AGG
AspEI 1189 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 2094 GCACATCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4228 GGATAC GTATCC(6/5)
BglI 5358 GCCAACTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1416 AGATCT A^GATCT
BsaBI 3539 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4967 ACCGGA [AT]^CCGG[AT]
BsrFI 3642 GCCGGT [GA]^CCGG[CT]
BssSI 863 CACGAG CACGAG(-5/-1)
BstAPI 1770 GCATTTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 446 CCTGAGG CC^T[ACGT]AGG
Cfr10I 3642 GCCGGT [GA]^CCGG[CT]
DrdI 3506 GACCTGCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1189 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 446 CCTGAGG CC^T[ACGT]AGG
EcoRI 2012 GAATTC G^AATTC
EcoRV 3488 GATATC GAT^ATC
FauI 1270 CCCGC CCCGC(4/6)
MamI 3539 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 4696 CCATGG C^CATGG
NdeI 847 CATATG CA^TATG
PaeR7I 3160 CTCGAG C^TCGAG
PshAI 2345 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1653 GCTCTTC GCTCTTC(1/4)
ScaI 5028 AGTACT AGT^ACT
SmaI 4188 CCCGGG CCC^GGG
SspI 1934 AATATT AAT^ATT
XhoI 3160 CTCGAG C^TCGAG
XmaCI 4188 CCCGGG C^CCGGG
XmaI 4188 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 3372 GGCGTC G[GA]^CG[CT]C
4136 GACGCC
AflII 4571 CTTAAG C^TTAAG
4666 CTTAAG
AflIII 2436 ACGTGT A^C[GA][CT]GT
5409 ACACGT
ApaI 963 GGGCCC GGGCC^C
4183 GGGCCC
AvaI 3160 CTCGAG C^[CT]CG[GA]G
4188 CCCGGG
AvrII 798 CCTAGG C^CTAGG
3382 CCTAGG
BalI 2431 TGGCCA TGG^CCA
4699 TGGCCA
BamHI 1055 GGATCC G^GATCC
3877 GGATCC
BbrPI 2435 CACGTG CAC^GTG
3259 CACGTG
BclI 2191 TGATCA T^GATCA
3928 TGATCA
BfrI 4571 CTTAAG C^TTAAG
4666 CTTAAG
BlnI 798 CCTAGG C^CTAGG
3382 CCTAGG
BsaHI 3372 GGCGTC G[GA]^CG[CT]C
4136 GACGCC
BsoBI 3160 CTCGAG C^[CT]CG[GA]G
4188 CCCGGG
BsrDI 2963 CATTGC GCAATG(2/0)
3982 CATTGC
Bst1107I 3695 GTATAC GTA^TAC
4819 GTATAC
BstZ17I 3695 GTATAC GTA^TAC
4819 GTATAC
DraIII 2732 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
3017 CACATCGTG
EcoNI 3070 CCTCCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4117 CCTGAATGAGG
FbaI 2191 TGATCA T^GATCA
3928 TGATCA
HgaI 4136 GACGC GACGC(5/10)
3373 GCGTC
Hin1I 3372 GGCGTC G[GA]^CG[CT]C
4136 GACGCC
MluNI 2431 TGGCCA TGG^CCA
4699 TGGCCA
MscI 2431 TGGCCA TGG^CCA
4699 TGGCCA
NspI 1789 ACATGC [GA]CATG^[CT]
3511 GCATGT
PmaCI 2435 CACGTG CAC^GTG
3259 CACGTG
PmlI 2435 CACGTG CAC^GTG
3259 CACGTG
SexAI 524 ACCTGGT A^CC[AT]GGT
1626 ACCTGGT
TaqI 3161 TCGA T^CGA
3906 TCGA
TthHB8I 3161 TCGA T^CGA
3906 TCGA
XcmI 4532 CCAAGCTGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5359 CCAACTTGGCCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspHI BspMI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MspI MunI
MvaI MwoI NciI NdeII NlaIII
NlaIV NsiI PflMI PleI PpuMI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XbaI XhoII XmnI