##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AhdI AocI AscI AseI AsnI
Asp718I AspEI AvrII BamHI BbrPI
BclI BfrI BlnI BsaBI BsiEI
BsiWI Bsp1407I BspDI BspLU11I BsrDI
BsrGI Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco52I Eco81I EcoRV
EcoT22I FbaI FseI HpaI KpnI
MamI MfeI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SalI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI Tru9I XbaI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AatII 4975 GACGTC GACGT^C
AccIII 1199 TCCGGA T^CCGGA
AflIII 4240 ACGCGT A^C[GA][CT]GT
Alw44I 3829 GTGCAC G^TGCAC
ApaLI 3829 GTGCAC G^TGCAC
AspI 4846 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 296 TGCGCA TGC^GCA
BcgI 1857 GCAGAGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1370 GTATCC GTATCC(6/5)
BsaAI 2702 TACGTG [CT]AC^GT[GA]
BsaWI 1199 TCCGGA [AT]^CCGG[AT]
BseAI 1199 TCCGGA T^CCGGA
BsmI 91 GAATGC GAATGC(1/-1)
BspEI 1199 TCCGGA T^CCGGA
BspHI 1296 TCATGA T^CATGA
EcoRI 2229 GAATTC G^AATTC
FspI 296 TGCGCA TGC^GCA
HincII 1006 GTTGAC GT[CT]^[GA]AC
HindII 1006 GTTGAC GT[CT]^[GA]AC
HindIII 394 AAGCTT A^AGCTT
MluI 4240 ACGCGT A^CGCGT
MroI 1199 TCCGGA T^CCGGA
PflFI 4846 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1296 TCATGA T^CATGA
SapI 4828 GAAGAGC GCTCTTC(1/4)
SbfI 3987 CCTGCAGG CCTGCA^GG
SexAI 4847 ACCAGGT A^CC[AT]GGT
Sse8387I 3987 CCTGCAGG CCTGCA^GG
Tth111I 4846 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
Aor51HI 1427 AGCGCT AGC^GCT
1861 AGCGCT
Asp700I 660 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3214 GAACTGCTTC
BssHII 1816 GCGCGC G^CGCGC
1818 GCGCGC
BstEII 2169 GGTGACC G^GT[ACGT]ACC
5010 GGTGACC
BstPI 2169 GGTGACC G^GT[ACGT]ACC
5010 GGTGACC
BstXI 3158 CCATCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5338 CCAAGGAGCTGG
Eco47III 1427 AGCGCT AGC^GCT
1861 AGCGCT
EcoO65I 2169 GGTGACC G^GT[ACGT]ACC
5010 GGTGACC
KspI 598 CCGCGG CCGC^GG
3401 CCGCGG
NaeI 997 GCCGGC GCC^GGC
2063 GCCGGC
NgoMIV 997 GCCGGC G^CCGGC
2063 GCCGGC
SacII 598 CCGCGG CCGC^GG
3401 CCGCGG
StuI 1484 AGGCCT AGG^CCT
5439 AGGCCT
XmnI 660 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3214 GAACTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PaeR7I PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoI XhoII XmaCI XmaI