################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AatII AccI AclI AhdI Alw44I AocI ApaLI AscI AseI AsnI AspEI BciVI BclI BsiEI BsiWI BsmBI Bsp1407I BspDI BspLU11I BsrGI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I Eco81I EcoT22I FbaI FseI KspI MluI NcoI NdeI NotI NruI NsiI NspV PacI PaeR7I PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfuI SgrAI Sse8387I SspBI SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 2277 GGTACC G^GTACC AccIII 3729 TCCGGA T^CCGGA AflII 1911 CTTAAG C^TTAAG AflIII 1347 ACACGT A^C[GA][CT]GT AgeI 1178 ACCGGT A^CCGGT Aor51HI 803 AGCGCT AGC^GCT Asp718I 2277 GGTACC G^GTACC AvrII 5110 CCTAGG C^CTAGG BamHI 3771 GGATCC G^GATCC BbrPI 1348 CACGTG CAC^GTG BfrI 1911 CTTAAG C^TTAAG BglII 1520 AGATCT A^GATCT BlnI 5110 CCTAGG C^CTAGG BsaBI 2514 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 3729 TCCGGA T^CCGGA BspEI 3729 TCCGGA T^CCGGA BspHI 1892 TCATGA T^CATGA BssHII 2350 GCGCGC G^CGCGC DraIII 1583 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2812 GACCTGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 803 AGCGCT AGC^GCT EcoRI 560 GAATTC G^AATTC EcoRV 258 GATATC GAT^ATC HincII 4268 GTTAAC GT[CT]^[GA]AC HindII 4268 GTTAAC GT[CT]^[GA]AC HindIII 2032 AAGCTT A^AGCTT HpaI 4268 GTTAAC GTT^AAC KpnI 2277 GGTACC GGTAC^C MamI 2514 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 4526 CAATTG C^AATTG MroI 3729 TCCGGA T^CCGGA MunI 4526 CAATTG C^AATTG NheI 5043 GCTAGC G^CTAGC PinAI 1178 ACCGGT A^CCGGT PmaCI 1348 CACGTG CAC^GTG PmlI 1348 CACGTG CAC^GTG RcaI 1892 TCATGA T^CATGA SapI 643 GAAGAGC GCTCTTC(1/4) ScaI 5305 AGTACT AGT^ACT SmaI 3537 CCCGGG CCC^GGG SnaBI 5383 TACGTA TAC^GTA SpeI 4935 ACTAGT A^CTAGT SphI 2786 GCATGC GCATG^C StuI 4892 AGGCCT AGG^CCT XbaI 483 TCTAGA T^CTAGA XmaCI 3537 CCCGGG C^CCGGG XmaI 3537 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcsI 193 GAATTT [GA]^AATT[CT] 560 GAATTC ApaI 1 GGGCCC GGGCC^C 4122 GGGCCC ApoI 193 GAATTT [GA]^AATT[CT] 560 GAATTC Asp700I 3204 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3234 GAAACGCTTC AspI 3821 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 5087 GACCCTGTC AviII 968 TGCGCA TGC^GCA 1760 TGCGCA BcgI 4775 CGAGCCTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1562 GCACTCAGGTCG BsrDI 341 CATTGC GCAATG(2/0) 2525 GCAATG BstEII 705 GGTCACC G^GT[ACGT]ACC 2631 GGTGACC BstPI 705 GGTCACC G^GT[ACGT]ACC 2631 GGTGACC EcoO65I 705 GGTCACC G^GT[ACGT]ACC 2631 GGTGACC FspI 968 TGCGCA TGC^GCA 1760 TGCGCA HgaI 1418 GACGC GACGC(5/10) 2016 GACGC NaeI 125 GCCGGC GCC^GGC 3464 GCCGGC NgoMIV 125 GCCGGC G^CCGGC 3464 GCCGGC PflFI 3821 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 5087 GACCCTGTC PshAI 3628 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4321 GACTCATGTC SexAI 2972 ACCAGGT A^CC[AT]GGT 5290 ACCAGGT SfiI 2038 GGCCACCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 2445 GGCCTGCCGGGCC Tth111I 3821 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 5087 GACCCTGTC XmnI 3204 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3234 GAAACGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII