##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AccI AclI AhdI Alw44I
AocI ApaLI AscI AseI AsnI
AspEI BciVI BclI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BsrGI
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco52I Eco81I EcoT22I FbaI FseI
KspI MluI NcoI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SfuI SgrAI
Sse8387I SspBI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 2277 GGTACC G^GTACC
AccIII 3729 TCCGGA T^CCGGA
AflII 1911 CTTAAG C^TTAAG
AflIII 1347 ACACGT A^C[GA][CT]GT
AgeI 1178 ACCGGT A^CCGGT
Aor51HI 803 AGCGCT AGC^GCT
Asp718I 2277 GGTACC G^GTACC
AvrII 5110 CCTAGG C^CTAGG
BamHI 3771 GGATCC G^GATCC
BbrPI 1348 CACGTG CAC^GTG
BfrI 1911 CTTAAG C^TTAAG
BglII 1520 AGATCT A^GATCT
BlnI 5110 CCTAGG C^CTAGG
BsaBI 2514 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3729 TCCGGA T^CCGGA
BspEI 3729 TCCGGA T^CCGGA
BspHI 1892 TCATGA T^CATGA
BssHII 2350 GCGCGC G^CGCGC
DraIII 1583 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2812 GACCTGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 803 AGCGCT AGC^GCT
EcoRI 560 GAATTC G^AATTC
EcoRV 258 GATATC GAT^ATC
HincII 4268 GTTAAC GT[CT]^[GA]AC
HindII 4268 GTTAAC GT[CT]^[GA]AC
HindIII 2032 AAGCTT A^AGCTT
HpaI 4268 GTTAAC GTT^AAC
KpnI 2277 GGTACC GGTAC^C
MamI 2514 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4526 CAATTG C^AATTG
MroI 3729 TCCGGA T^CCGGA
MunI 4526 CAATTG C^AATTG
NheI 5043 GCTAGC G^CTAGC
PinAI 1178 ACCGGT A^CCGGT
PmaCI 1348 CACGTG CAC^GTG
PmlI 1348 CACGTG CAC^GTG
RcaI 1892 TCATGA T^CATGA
SapI 643 GAAGAGC GCTCTTC(1/4)
ScaI 5305 AGTACT AGT^ACT
SmaI 3537 CCCGGG CCC^GGG
SnaBI 5383 TACGTA TAC^GTA
SpeI 4935 ACTAGT A^CTAGT
SphI 2786 GCATGC GCATG^C
StuI 4892 AGGCCT AGG^CCT
XbaI 483 TCTAGA T^CTAGA
XmaCI 3537 CCCGGG C^CCGGG
XmaI 3537 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 193 GAATTT [GA]^AATT[CT]
560 GAATTC
ApaI 1 GGGCCC GGGCC^C
4122 GGGCCC
ApoI 193 GAATTT [GA]^AATT[CT]
560 GAATTC
Asp700I 3204 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3234 GAAACGCTTC
AspI 3821 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5087 GACCCTGTC
AviII 968 TGCGCA TGC^GCA
1760 TGCGCA
BcgI 4775 CGAGCCTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1562 GCACTCAGGTCG
BsrDI 341 CATTGC GCAATG(2/0)
2525 GCAATG
BstEII 705 GGTCACC G^GT[ACGT]ACC
2631 GGTGACC
BstPI 705 GGTCACC G^GT[ACGT]ACC
2631 GGTGACC
EcoO65I 705 GGTCACC G^GT[ACGT]ACC
2631 GGTGACC
FspI 968 TGCGCA TGC^GCA
1760 TGCGCA
HgaI 1418 GACGC GACGC(5/10)
2016 GACGC
NaeI 125 GCCGGC GCC^GGC
3464 GCCGGC
NgoMIV 125 GCCGGC G^CCGGC
3464 GCCGGC
PflFI 3821 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5087 GACCCTGTC
PshAI 3628 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4321 GACTCATGTC
SexAI 2972 ACCAGGT A^CC[AT]GGT
5290 ACCAGGT
SfiI 2038 GGCCACCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2445 GGCCTGCCGGGCC
Tth111I 3821 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5087 GACCCTGTC
XmnI 3204 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3234 GAAACGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII