##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
AseI AsnI Asp700I AspEI AviII
AvrII BclI BfrI BlnI BsaBI
BsiEI BsiWI BsmBI BspDI ClaI
DraI DrdI EagI Eam1105I EclXI
Eco52I EcoRV EcoT22I FbaI FseI
FspI HindIII MamI MfeI MluI
MunI NaeI NgoMIV NotI NruI
NsiI PacI PinAI PmeI PshBI
Psp1406I PvuI SalI SbfI ScaI
SfiI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 4356 GGTACC G^GTACC
AccIII 17 TCCGGA T^CCGGA
AscI 599 GGCGCGCC GG^CGCGCC
Asp718I 4356 GGTACC G^GTACC
AspI 4187 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 477 GGCGCC GGCGC^C
BciVI 3101 GGATAC GTATCC(6/5)
BglI 947 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 17 TCCGGA T^CCGGA
BsmI 4428 GCATTC GAATGC(1/-1)
Bsp1407I 2536 TGTACA T^GTACA
BspEI 17 TCCGGA T^CCGGA
BspHI 1311 TCATGA T^CATGA
BsrGI 2536 TGTACA T^GTACA
BssHII 600 GCGCGC G^CGCGC
BssSI 1227 CACGAG CACGAG(-5/-1)
Bst1107I 1106 GTATAC GTA^TAC
BstAPI 4225 GCAGACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1701 TTCGAA TT^CGAA
BstXI 1813 CCATCTGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1106 GTATAC GTA^TAC
CpoI 19 CGGACCG CG^G[AT]CCG
EcoRI 3552 GAATTC G^AATTC
HpaI 4072 GTTAAC GTT^AAC
KasI 477 GGCGCC G^GCGCC
KpnI 4356 GGTACC GGTAC^C
KspI 28 CCGCGG CCGC^GG
MroI 17 TCCGGA T^CCGGA
NarI 477 GGCGCC GG^CGCC
NdeI 2403 CATATG CA^TATG
NheI 2613 GCTAGC G^CTAGC
NspV 1701 TTCGAA TT^CGAA
PaeR7I 1652 CTCGAG C^TCGAG
PflFI 4187 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1311 TCATGA T^CATGA
RsrII 19 CGGACCG CG^G[AT]CCG
SacII 28 CCGCGG CCGC^GG
SfoI 477 GGCGCC GGC^GCC
SfuI 1701 TTCGAA TT^CGAA
SspBI 2536 TGTACA T^GTACA
Tth111I 4187 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 799 TCTAGA T^CTAGA
XhoI 1652 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 2509 GAATTT [GA]^AATT[CT]
3552 GAATTC
Alw44I 4418 GTGCAC G^TGCAC
4570 GTGCAC
Aor51HI 568 AGCGCT AGC^GCT
3661 AGCGCT
ApaI 84 GGGCCC GGGCC^C
4248 GGGCCC
ApaLI 4418 GTGCAC G^TGCAC
4570 GTGCAC
ApoI 2509 GAATTT [GA]^AATT[CT]
3552 GAATTC
BlpI 2462 GCTGAGC GC^T[ACGT]AGC
4884 GCTGAGC
Bpu1102I 2462 GCTGAGC GC^T[ACGT]AGC
4884 GCTGAGC
BsaI 93 GAGACC GGTCTC(1/5)
4679 GGTCTC
BsaWI 17 TCCGGA [AT]^CCGG[AT]
878 TCCGGT
BspLU11I 747 ACATGT A^CATGT
2917 ACATGT
BsrDI 2380 CATTGC GCAATG(2/0)
3970 GCAATG
BsrFI 340 ACCGGC [GA]^CCGG[CT]
870 GCCGGT
BstEII 2642 GGTGACC G^GT[ACGT]ACC
4556 GGTCACC
BstPI 2642 GGTGACC G^GT[ACGT]ACC
4556 GGTCACC
CelII 2462 GCTGAGC GC^T[ACGT]AGC
4884 GCTGAGC
Cfr10I 340 ACCGGC [GA]^CCGG[CT]
870 GCCGGT
DraIII 4151 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
4559 CACCAGGTG
Eco47III 568 AGCGCT AGC^GCT
3661 AGCGCT
EcoO65I 2642 GGTGACC G^GT[ACGT]ACC
4556 GGTCACC
PshAI 1376 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4712 GACACGTGTC
SacI 986 GAGCTC GAGCT^C
3653 GAGCTC
SapI 4932 GAAGAGC GCTCTTC(1/4)
4411 GCTCTTC
SexAI 4540 ACCAGGT A^CC[AT]GGT
4560 ACCAGGT
SmaI 69 CCCGGG CCC^GGG
145 CCCGGG
StuI 219 AGGCCT AGG^CCT
3603 AGGCCT
XmaCI 69 CCCGGG C^CCGGG
145 CCCGGG
XmaI 69 CCCGGG C^CCGGG
145 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbrPI BbsI BbvCI
BbvI BcgI BcnI BfaI BglII
BmyI BpmI BpuAI BsaAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII