################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AatII AclI AflII AgeI AhdI AseI AsnI Asp700I AspEI AviII AvrII BclI BfrI BlnI BsaBI BsiEI BsiWI BsmBI BspDI ClaI DraI DrdI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FbaI FseI FspI HindIII MamI MfeI MluI MunI NaeI NgoMIV NotI NruI NsiI PacI PinAI PmeI PshBI Psp1406I PvuI SalI SbfI ScaI SfiI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 4356 GGTACC G^GTACC AccIII 17 TCCGGA T^CCGGA AscI 599 GGCGCGCC GG^CGCGCC Asp718I 4356 GGTACC G^GTACC AspI 4187 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 477 GGCGCC GGCGC^C BciVI 3101 GGATAC GTATCC(6/5) BglI 947 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BseAI 17 TCCGGA T^CCGGA BsmI 4428 GCATTC GAATGC(1/-1) Bsp1407I 2536 TGTACA T^GTACA BspEI 17 TCCGGA T^CCGGA BspHI 1311 TCATGA T^CATGA BsrGI 2536 TGTACA T^GTACA BssHII 600 GCGCGC G^CGCGC BssSI 1227 CACGAG CACGAG(-5/-1) Bst1107I 1106 GTATAC GTA^TAC BstAPI 4225 GCAGACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1701 TTCGAA TT^CGAA BstXI 1813 CCATCTGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1106 GTATAC GTA^TAC CpoI 19 CGGACCG CG^G[AT]CCG EcoRI 3552 GAATTC G^AATTC HpaI 4072 GTTAAC GTT^AAC KasI 477 GGCGCC G^GCGCC KpnI 4356 GGTACC GGTAC^C KspI 28 CCGCGG CCGC^GG MroI 17 TCCGGA T^CCGGA NarI 477 GGCGCC GG^CGCC NdeI 2403 CATATG CA^TATG NheI 2613 GCTAGC G^CTAGC NspV 1701 TTCGAA TT^CGAA PaeR7I 1652 CTCGAG C^TCGAG PflFI 4187 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 1311 TCATGA T^CATGA RsrII 19 CGGACCG CG^G[AT]CCG SacII 28 CCGCGG CCGC^GG SfoI 477 GGCGCC GGC^GCC SfuI 1701 TTCGAA TT^CGAA SspBI 2536 TGTACA T^GTACA Tth111I 4187 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 799 TCTAGA T^CTAGA XhoI 1652 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 2509 GAATTT [GA]^AATT[CT] 3552 GAATTC Alw44I 4418 GTGCAC G^TGCAC 4570 GTGCAC Aor51HI 568 AGCGCT AGC^GCT 3661 AGCGCT ApaI 84 GGGCCC GGGCC^C 4248 GGGCCC ApaLI 4418 GTGCAC G^TGCAC 4570 GTGCAC ApoI 2509 GAATTT [GA]^AATT[CT] 3552 GAATTC BlpI 2462 GCTGAGC GC^T[ACGT]AGC 4884 GCTGAGC Bpu1102I 2462 GCTGAGC GC^T[ACGT]AGC 4884 GCTGAGC BsaI 93 GAGACC GGTCTC(1/5) 4679 GGTCTC BsaWI 17 TCCGGA [AT]^CCGG[AT] 878 TCCGGT BspLU11I 747 ACATGT A^CATGT 2917 ACATGT BsrDI 2380 CATTGC GCAATG(2/0) 3970 GCAATG BsrFI 340 ACCGGC [GA]^CCGG[CT] 870 GCCGGT BstEII 2642 GGTGACC G^GT[ACGT]ACC 4556 GGTCACC BstPI 2642 GGTGACC G^GT[ACGT]ACC 4556 GGTCACC CelII 2462 GCTGAGC GC^T[ACGT]AGC 4884 GCTGAGC Cfr10I 340 ACCGGC [GA]^CCGG[CT] 870 GCCGGT DraIII 4151 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG 4559 CACCAGGTG Eco47III 568 AGCGCT AGC^GCT 3661 AGCGCT EcoO65I 2642 GGTGACC G^GT[ACGT]ACC 4556 GGTCACC PshAI 1376 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4712 GACACGTGTC SacI 986 GAGCTC GAGCT^C 3653 GAGCTC SapI 4932 GAAGAGC GCTCTTC(1/4) 4411 GCTCTTC SexAI 4540 ACCAGGT A^CC[AT]GGT 4560 ACCAGGT SmaI 69 CCCGGG CCC^GGG 145 CCCGGG StuI 219 AGGCCT AGG^CCT 3603 AGGCCT XmaCI 69 CCCGGG C^CCGGG 145 CCCGGG XmaI 69 CCCGGG C^CCGGG 145 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 128 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI AocI AspHI AvaI AvaII BalI BamHI BanI BanII BbrPI BbsI BbvCI BbvI BcgI BcnI BfaI BglII BmyI BpmI BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII