##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AccIII AclI Alw44I
ApaLI AscI AseI AsnI Asp718I
AspI AvrII BbeI BbrPI BlnI
BsaAI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BssHII Bst1107I BstEII
BstPI BstZ17I ClaI CpoI EagI
EclXI Eco52I EcoO65I FseI HpaI
KasI KpnI KspI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SbfI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 9 GGCGTC G[GA]^CG[CT]C
AflII 1004 CTTAAG C^TTAAG
AhdI 5219 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4590 AGCGCT AGC^GCT
Asp700I 5352 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 5219 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 838 GCATAGAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1004 CTTAAG C^TTAAG
BglII 708 AGATCT A^GATCT
BsaBI 2536 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 9 GGCGTC G[GA]^CG[CT]C
BsrBI 4571 GAGCGG CCGCTC(-3/-3)
BssSI 846 CTCGTG CACGAG(-5/-1)
BstBI 5532 TTCGAA TT^CGAA
BstXI 1611 CCAGGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 2059 GACAGTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 5219 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4590 AGCGCT AGC^GCT
EcoRV 2609 GATATC GAT^ATC
FauI 3861 CCCGC CCCGC(4/6)
Hin1I 9 GGCGTC G[GA]^CG[CT]C
HindIII 4411 AAGCTT A^AGCTT
MamI 2536 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 4540 CATATG CA^TATG
NspV 5532 TTCGAA TT^CGAA
SacI 2653 GAGCTC GAGCT^C
SapI 217 GAAGAGC GCTCTTC(1/4)
ScaI 3521 AGTACT AGT^ACT
SexAI 2117 ACCAGGT A^CC[AT]GGT
SfuI 5532 TTCGAA TT^CGAA
SmaI 1548 CCCGGG CCC^GGG
XmaCI 1548 CCCGGG C^CCGGG
XmaI 1548 CCCGGG C^CCGGG
XmnI 5352 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 521 CGCG CG^CG
859 CGCG
AflIII 2315 ACATGT A^C[GA][CT]GT
5055 ACATGT
AocI 1748 CCTCAGG CC^T[ACGT]AGG
4628 CCTCAGG
AviII 3879 TGCGCA TGC^GCA
4215 TGCGCA
BamHI 1539 GGATCC G^GATCC
1852 GGATCC
BciVI 4724 GGATAC GTATCC(6/5)
2661 GTATCC
BglI 911 GCCTGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1807 GCCCATGGGGC
Bsp1407I 2216 TGTACA T^GTACA
4514 TGTACA
BspLU11I 2315 ACATGT A^CATGT
5055 ACATGT
BsrGI 2216 TGTACA T^GTACA
4514 TGTACA
BstAPI 1580 GCAACCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4634 GCACGCCCTGC
BstUI 521 CGCG CG^CG
859 CGCG
Bsu36I 1748 CCTCAGG CC^T[ACGT]AGG
4628 CCTCAGG
DraI 4859 TTTAAA TTT^AAA
5617 TTTAAA
DraIII 3477 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
5657 CACTTTGTG
Eco81I 1748 CCTCAGG CC^T[ACGT]AGG
4628 CCTCAGG
EcoRI 3303 GAATTC G^AATTC
4837 GAATTC
EcoT22I 2746 ATGCAT ATGCA^T
4657 ATGCAT
FspI 3879 TGCGCA TGC^GCA
4215 TGCGCA
MvnI 521 CGCG CG^CG
859 CGCG
NsiI 2746 ATGCAT ATGCA^T
4657 ATGCAT
SalI 1013 GTCGAC G^TCGAC
4089 GTCGAC
SspBI 2216 TGTACA T^GTACA
4514 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccI AciI AcsI AfaI AgeI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BclI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PinAI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XcmI
XhoII