##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I BbeI BbrPI BglII
BsaAI BsaBI BseAI BsiEI BsiWI
BspDI BspEI BspHI BssHII Bst1107I
BstBI BstZ17I ClaI DraI EagI
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FseI HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NgoMIV NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI SacII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI SwaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AflII 3814 CTTAAG C^TTAAG
AflIII 2791 ACATGT A^C[GA][CT]GT
AhdI 2622 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 3544 GGGCCC GGGCC^C
AspEI 2622 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2946 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3163 TGCGCA TGC^GCA
AvrII 4222 CCTAGG C^CTAGG
BamHI 2164 GGATCC G^GATCC
BcgI 1323 CGACTCTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2690 GGATAC GTATCC(6/5)
BclI 295 TGATCA T^GATCA
BfrI 3814 CTTAAG C^TTAAG
BlnI 4222 CCTAGG C^CTAGG
Bsp1407I 2392 TGTACA T^GTACA
BspLU11I 2791 ACATGT A^CATGT
BsrBI 1187 CCGCTC CCGCTC(-3/-3)
BsrGI 2392 TGTACA T^GTACA
BssSI 1050 CACGAG CACGAG(-5/-1)
BstAPI 333 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1154 GGTCACC G^GT[ACGT]ACC
BstPI 1154 GGTCACC G^GT[ACGT]ACC
CpoI 2950 CGGTCCG CG^G[AT]CCG
DrdI 2430 GACCAGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2622 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1154 GGTCACC G^GT[ACGT]ACC
FauI 343 CCCGC CCCGC(4/6)
FbaI 295 TGATCA T^GATCA
FspI 3163 TGCGCA TGC^GCA
HincII 957 GTTGAC GT[CT]^[GA]AC
HindII 957 GTTGAC GT[CT]^[GA]AC
HindIII 1608 AAGCTT A^AGCTT
MaeII 685 ACGT A^CGT
NdeI 125 CATATG CA^TATG
PaeR7I 1007 CTCGAG C^TCGAG
PflFI 2946 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1005 GACTCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2031 CTGCAG CTGCA^G
RsrII 2950 CGGTCCG CG^G[AT]CCG
SapI 798 GAAGAGC GCTCTTC(1/4)
SexAI 3781 ACCTGGT A^CC[AT]GGT
SspBI 2392 TGTACA T^GTACA
StuI 3701 AGGCCT AGG^CCT
Tth111I 2946 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4472 TCTAGA T^CTAGA
XhoI 1007 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 579 GTCTAC GT^[AC][GT]AC
3371 GTAGAC
AcyI 1498 GACGCC G[GA]^CG[CT]C
2421 GGCGTC
Asp700I 740 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4396 GAATCTCTTC
AvaI 1007 CTCGAG C^[CT]CG[GA]G
2596 CTCGGG
BsaHI 1498 GACGCC G[GA]^CG[CT]C
2421 GGCGTC
BsaWI 2809 TCCGGT [AT]^CCGG[AT]
4148 TCCGGT
BsmI 2822 GAATGC GAATGC(1/-1)
110 GCATTC
BsoBI 1007 CTCGAG C^[CT]CG[GA]G
2596 CTCGGG
BsrDI 1634 CATTGC GCAATG(2/0)
2776 GCAATG
BsrFI 3276 GCCGGT [GA]^CCGG[CT]
3389 GCCGGT
BstXI 890 CCACAGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2650 CCACATCGCTGG
Cfr10I 3276 GCCGGT [GA]^CCGG[CT]
3389 GCCGGT
DraIII 1830 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2849 CACCGTGTG
EcoNI 2030 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3249 CCTGCGTCAGG
Hin1I 1498 GACGCC G[GA]^CG[CT]C
2421 GGCGTC
NcoI 2305 CCATGG C^CATGG
2416 CCATGG
ScaI 1474 AGTACT AGT^ACT
2365 AGTACT
SfcI 2031 CTGCAG C^T[GA][CT]AG
3849 CTACAG
SphI 312 GCATGC GCATG^C
3001 GCATGC
XmnI 740 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4396 GAATCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NheI NlaIII
NlaIV NspI PflMI PleI PpuMI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII