##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AccIII AclI AhdI AscI AseI
AsnI AspEI AspI AviII BalI
BamHI BbvCI BciVI BsaBI BseAI
BsiWI BspDI BspEI BsrBI BssHII
BstBI ClaI CpoI EagI Eam1105I
EclXI Eco52I FseI FspI KspI
MamI MluI MluNI MroI MscI
NaeI NcoI NgoMIV NotI NruI
NspV PaeR7I PflFI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SfiI SfuI SgrAI
SnaBI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 5418 GACGTC GACGT^C
AgeI 4409 ACCGGT A^CCGGT
Aor51HI 4369 AGCGCT AGC^GCT
AvrII 4398 CCTAGG C^CTAGG
BbeI 926 GGCGCC GGCGC^C
BbrPI 6214 CACGTG CAC^GTG
BclI 4209 TGATCA T^GATCA
BlnI 4398 CCTAGG C^CTAGG
BsaAI 6214 CACGTG [CT]AC^GT[GA]
BsaWI 4409 ACCGGT [AT]^CCGG[AT]
BsiEI 6062 CGACCG CG[GA][CT]^CG
BsmBI 504 GAGACG CGTCTC(1/5)
BsmI 74 GAATGC GAATGC(1/-1)
BspHI 4212 TCATGA T^CATGA
BssSI 764 CACGAG CACGAG(-5/-1)
BstXI 4948 CCAGTCAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 206 GACATCATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1060 CCGTGG C^C[GA][CT]GG
EaeI 912 TGGCCG [CT]^GGCC[GA]
Eco47III 4369 AGCGCT AGC^GCT
EcoNI 1604 CCTGACTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3848 GATATC GAT^ATC
FbaI 4209 TGATCA T^GATCA
KasI 926 GGCGCC G^GCGCC
MfeI 4953 CAATTG C^AATTG
MunI 4953 CAATTG C^AATTG
NarI 926 GGCGCC GG^CGCC
NdeI 1289 CATATG CA^TATG
PacI 3812 TTAATTAA TTAAT^TAA
PinAI 4409 ACCGGT A^CCGGT
PmaCI 6214 CACGTG CAC^GTG
PmlI 6214 CACGTG CAC^GTG
RcaI 4212 TCATGA T^CATGA
SapI 855 GCTCTTC GCTCTTC(1/4)
SbfI 1108 CCTGCAGG CCTGCA^GG
SexAI 2534 ACCAGGT A^CC[AT]GGT
SfoI 926 GGCGCC GGC^GCC
Sse8387I 1108 CCTGCAGG CCTGCA^GG
SspI 6356 AATATT AAT^ATT
XbaI 2970 TCTAGA T^CTAGA
XcmI 5764 CCAGATTTGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 1203 GGTACC G^GTACC
1430 GGTACC
AccII 1338 CGCG CG^CG
1642 CGCG
AcyI 926 GGCGCC G[GA]^CG[CT]C
5418 GACGTC
AflII 3364 CTTAAG C^TTAAG
3731 CTTAAG
Alw44I 460 GTGCAC G^TGCAC
2680 GTGCAC
AocI 614 CCTCAGG CC^T[ACGT]AGG
5984 CCTCAGG
ApaLI 460 GTGCAC G^TGCAC
2680 GTGCAC
Asp718I 1203 GGTACC G^GTACC
1430 GGTACC
BcgI 1534 CGAAAGTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
703 GCACTCAGATCG
BfrI 3364 CTTAAG C^TTAAG
3731 CTTAAG
BglI 2813 GCCCCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3479 GCCCCGGTGGC
BglII 2894 AGATCT A^GATCT
5263 AGATCT
BsaHI 926 GGCGCC G[GA]^CG[CT]C
5418 GACGTC
BsaI 5996 GAGACC GGTCTC(1/5)
4589 GGTCTC
Bsp1407I 4142 TGTACA T^GTACA
4572 TGTACA
BsrDI 4130 CATTGC GCAATG(2/0)
4769 CATTGC
BsrFI 834 ACCGGC [GA]^CCGG[CT]
4409 ACCGGT
BsrGI 4142 TGTACA T^GTACA
4572 TGTACA
Bst1107I 3608 GTATAC GTA^TAC
5194 GTATAC
BstAPI 3590 GCAGCTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5894 GCAAGCTCTGC
BstEII 1468 GGTCACC G^GT[ACGT]ACC
6187 GGTAACC
BstPI 1468 GGTCACC G^GT[ACGT]ACC
6187 GGTAACC
BstUI 1338 CGCG CG^CG
1642 CGCG
BstZ17I 3608 GTATAC GTA^TAC
5194 GTATAC
Bsu36I 614 CCTCAGG CC^T[ACGT]AGG
5984 CCTCAGG
Cfr10I 834 ACCGGC [GA]^CCGG[CT]
4409 ACCGGT
Eco81I 614 CCTCAGG CC^T[ACGT]AGG
5984 CCTCAGG
EcoO65I 1468 GGTCACC G^GT[ACGT]ACC
6187 GGTAACC
EcoRI 4298 GAATTC G^AATTC
6385 GAATTC
HgaI 5402 GACGC GACGC(5/10)
1643 GCGTC
Hin1I 926 GGCGCC G[GA]^CG[CT]C
5418 GACGTC
HpaI 2943 GTTAAC GTT^AAC
3552 GTTAAC
KpnI 1203 GGTACC GGTAC^C
1430 GGTACC
MvnI 1338 CGCG CG^CG
1642 CGCG
PflMI 1683 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4001 CCATGTCCTGG
PstI 1109 CTGCAG CTGCA^G
2718 CTGCAG
SmaI 1176 CCCGGG CCC^GGG
1462 CCCGGG
SpeI 6114 ACTAGT A^CTAGT
6254 ACTAGT
SspBI 4142 TGTACA T^GTACA
4572 TGTACA
Van91I 1683 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4001 CCATGTCCTGG
XmaCI 1176 CCCGGG C^CCGGG
1462 CCCGGG
XmaI 1176 CCCGGG C^CCGGG
1462 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspLU11I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII EarI EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NheI NlaIII NlaIV
NsiI NspI PleI PvuII RsaI
SacI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI