################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AccI AclI AflII AgeI AhdI AscI AseI AsnI AspEI AspI AvrII BbeI BciVI BclI BfrI BlnI BsaBI BsiEI BsiWI BspDI BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoRI EcoRV EcoT22I FbaI FseI HpaI KasI MamI MfeI MluI MunI NarI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacI SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AatII 3804 GACGTC GACGT^C Acc65I 408 GGTACC G^GTACC AccIII 3890 TCCGGA T^CCGGA AcsI 5279 AAATTT [GA]^AATT[CT] Alw44I 4214 GTGCAC G^TGCAC Aor51HI 541 AGCGCT AGC^GCT ApaLI 4214 GTGCAC G^TGCAC ApoI 5279 AAATTT [GA]^AATT[CT] Asp718I 408 GGTACC G^GTACC AviII 2563 TGCGCA TGC^GCA BamHI 3887 GGATCC G^GATCC BseAI 3890 TCCGGA T^CCGGA BsmBI 3063 GAGACG CGTCTC(1/5) BspEI 3890 TCCGGA T^CCGGA BspHI 2645 TCATGA T^CATGA BssHII 3784 GCGCGC G^CGCGC DrdI 3282 GACAAGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 541 AGCGCT AGC^GCT FspI 2563 TGCGCA TGC^GCA HindIII 3458 AAGCTT A^AGCTT KpnI 408 GGTACC GGTAC^C MroI 3890 TCCGGA T^CCGGA NaeI 1045 GCCGGC GCC^GGC NdeI 3450 CATATG CA^TATG NgoMIV 1045 GCCGGC G^CCGGC NheI 2549 GCTAGC G^CTAGC PshAI 2984 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 2645 TCATGA T^CATGA ScaI 760 AGTACT AGT^ACT SpeI 5458 ACTAGT A^CTAGT SspI 1608 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AflIII 2034 ACATGT A^C[GA][CT]GT 4774 ACATGT AocI 1698 CCTGAGG CC^T[ACGT]AGG 4660 CCTGAGG Asp700I 470 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1166 GAATGCGTTC BbrPI 4109 CACGTG CAC^GTG 4211 CACGTG BglII 2975 AGATCT A^GATCT 3502 AGATCT BlpI 7 GCTCAGC GC^T[ACGT]AGC 2496 GCTGAGC Bpu1102I 7 GCTCAGC GC^T[ACGT]AGC 2496 GCTGAGC BsaWI 3890 TCCGGA [AT]^CCGG[AT] 4247 ACCGGA BsmI 1000 GAATGC GAATGC(1/-1) 1166 GAATGC BspLU11I 2034 ACATGT A^CATGT 4774 ACATGT BsrFI 1045 GCCGGC [GA]^CCGG[CT] 2125 ACCGGC BstEII 485 GGTGACC G^GT[ACGT]ACC 2276 GGTCACC BstPI 485 GGTGACC G^GT[ACGT]ACC 2276 GGTCACC Bsu36I 1698 CCTGAGG CC^T[ACGT]AGG 4660 CCTGAGG CelII 7 GCTCAGC GC^T[ACGT]AGC 2496 GCTGAGC Cfr10I 1045 GCCGGC [GA]^CCGG[CT] 2125 ACCGGC Eco81I 1698 CCTGAGG CC^T[ACGT]AGG 4660 CCTGAGG EcoO65I 485 GGTGACC G^GT[ACGT]ACC 2276 GGTCACC HincII 417 GTCAAC GT[CT]^[GA]AC 4134 GTCAAC HindII 417 GTCAAC GT[CT]^[GA]AC 4134 GTCAAC KspI 1727 CCGCGG CCGC^GG 3270 CCGCGG PaeR7I 642 CTCGAG C^TCGAG 5158 CTCGAG PflMI 1217 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3143 CCACACCCTGG PmaCI 4109 CACGTG CAC^GTG 4211 CACGTG PmlI 4109 CACGTG CAC^GTG 4211 CACGTG SacII 1727 CCGCGG CCGC^GG 3270 CCGCGG SapI 170 GCTCTTC GCTCTTC(1/4) 4151 GCTCTTC SexAI 3047 ACCTGGT A^CC[AT]GGT 4824 ACCAGGT Van91I 1217 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3143 CCACACCCTGG XbaI 1715 TCTAGA T^CTAGA 5134 TCTAGA XhoI 642 CTCGAG C^TCGAG 5158 CTCGAG XmnI 470 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1166 GAATGCGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII