##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AccI AclI AflII AgeI AhdI
AscI AseI AsnI AspEI AspI
AvrII BbeI BciVI BclI BfrI
BlnI BsaBI BsiEI BsiWI BspDI
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI HpaI KasI MamI MfeI
MluI MunI NarI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacI SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AatII 3804 GACGTC GACGT^C
Acc65I 408 GGTACC G^GTACC
AccIII 3890 TCCGGA T^CCGGA
AcsI 5279 AAATTT [GA]^AATT[CT]
Alw44I 4214 GTGCAC G^TGCAC
Aor51HI 541 AGCGCT AGC^GCT
ApaLI 4214 GTGCAC G^TGCAC
ApoI 5279 AAATTT [GA]^AATT[CT]
Asp718I 408 GGTACC G^GTACC
AviII 2563 TGCGCA TGC^GCA
BamHI 3887 GGATCC G^GATCC
BseAI 3890 TCCGGA T^CCGGA
BsmBI 3063 GAGACG CGTCTC(1/5)
BspEI 3890 TCCGGA T^CCGGA
BspHI 2645 TCATGA T^CATGA
BssHII 3784 GCGCGC G^CGCGC
DrdI 3282 GACAAGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 541 AGCGCT AGC^GCT
FspI 2563 TGCGCA TGC^GCA
HindIII 3458 AAGCTT A^AGCTT
KpnI 408 GGTACC GGTAC^C
MroI 3890 TCCGGA T^CCGGA
NaeI 1045 GCCGGC GCC^GGC
NdeI 3450 CATATG CA^TATG
NgoMIV 1045 GCCGGC G^CCGGC
NheI 2549 GCTAGC G^CTAGC
PshAI 2984 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2645 TCATGA T^CATGA
ScaI 760 AGTACT AGT^ACT
SpeI 5458 ACTAGT A^CTAGT
SspI 1608 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AflIII 2034 ACATGT A^C[GA][CT]GT
4774 ACATGT
AocI 1698 CCTGAGG CC^T[ACGT]AGG
4660 CCTGAGG
Asp700I 470 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1166 GAATGCGTTC
BbrPI 4109 CACGTG CAC^GTG
4211 CACGTG
BglII 2975 AGATCT A^GATCT
3502 AGATCT
BlpI 7 GCTCAGC GC^T[ACGT]AGC
2496 GCTGAGC
Bpu1102I 7 GCTCAGC GC^T[ACGT]AGC
2496 GCTGAGC
BsaWI 3890 TCCGGA [AT]^CCGG[AT]
4247 ACCGGA
BsmI 1000 GAATGC GAATGC(1/-1)
1166 GAATGC
BspLU11I 2034 ACATGT A^CATGT
4774 ACATGT
BsrFI 1045 GCCGGC [GA]^CCGG[CT]
2125 ACCGGC
BstEII 485 GGTGACC G^GT[ACGT]ACC
2276 GGTCACC
BstPI 485 GGTGACC G^GT[ACGT]ACC
2276 GGTCACC
Bsu36I 1698 CCTGAGG CC^T[ACGT]AGG
4660 CCTGAGG
CelII 7 GCTCAGC GC^T[ACGT]AGC
2496 GCTGAGC
Cfr10I 1045 GCCGGC [GA]^CCGG[CT]
2125 ACCGGC
Eco81I 1698 CCTGAGG CC^T[ACGT]AGG
4660 CCTGAGG
EcoO65I 485 GGTGACC G^GT[ACGT]ACC
2276 GGTCACC
HincII 417 GTCAAC GT[CT]^[GA]AC
4134 GTCAAC
HindII 417 GTCAAC GT[CT]^[GA]AC
4134 GTCAAC
KspI 1727 CCGCGG CCGC^GG
3270 CCGCGG
PaeR7I 642 CTCGAG C^TCGAG
5158 CTCGAG
PflMI 1217 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3143 CCACACCCTGG
PmaCI 4109 CACGTG CAC^GTG
4211 CACGTG
PmlI 4109 CACGTG CAC^GTG
4211 CACGTG
SacII 1727 CCGCGG CCGC^GG
3270 CCGCGG
SapI 170 GCTCTTC GCTCTTC(1/4)
4151 GCTCTTC
SexAI 3047 ACCTGGT A^CC[AT]GGT
4824 ACCAGGT
Van91I 1217 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3143 CCACACCCTGG
XbaI 1715 TCTAGA T^CTAGA
5134 TCTAGA
XhoI 642 CTCGAG C^TCGAG
5158 CTCGAG
XmnI 470 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1166 GAATGCGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrBI
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII