################################## # NO CUT ENZYMES ( 54 enzymes) # ################################## Acc65I AccIII AflII AgeI AscI Asp718I BbeI BfrI BlpI Bpu1102I BsaBI BseAI BsiWI BspDI BspEI BssHII BssSI BstBI CelII ClaI CpoI DrdI EcoRI EcoRV EcoT22I FseI KasI KpnI KspI MamI MroI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PinAI PmeI PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SnaBI SpeI Sse8387I ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 4946 GACGTC GACGT^C AhdI 4017 GACTCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 3357 AGCGCT AGC^GCT AseI 3417 ATTAAT AT^TAAT AsnI 3417 ATTAAT AT^TAAT Asp700I 90 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 4017 GACTCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2060 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 70 TGCGCA TGC^GCA AvrII 609 CCTAGG C^CTAGG BamHI 1796 GGATCC G^GATCC BbvCI 2512 CCTCAGC CCTCAGC(-5/-2) BcgI 5390 GCATCCATCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 58 GCCGTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 4255 AGATCT A^GATCT BlnI 609 CCTAGG C^CTAGG BsiEI 848 CGGCCG CG[GA][CT]^CG BsmBI 2841 CGTCTC CGTCTC(1/5) BspHI 1946 TCATGA T^CATGA BsrDI 3119 CATTGC GCAATG(2/0) DraI 5258 TTTAAA TTT^AAA DsaI 4941 CCACGG C^C[GA][CT]GG EagI 848 CGGCCG C^GGCCG Eam1105I 4017 GACTCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 848 CGGCCG C^GGCCG Eco47III 3357 AGCGCT AGC^GCT Eco52I 848 CGGCCG C^GGCCG EcoNI 3910 CCTTCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 70 TGCGCA TGC^GCA MfeI 4362 CAATTG C^AATTG MluI 5410 ACGCGT A^CGCGT MunI 4362 CAATTG C^AATTG PacI 3389 TTAATTAA TTAAT^TAA PflFI 2060 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 1086 GACCCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3417 ATTAAT AT^TAAT RcaI 1946 TCATGA T^CATGA ScaI 5564 AGTACT AGT^ACT SexAI 4489 ACCTGGT A^CC[AT]GGT SphI 1005 GCATGC GCATG^C SwaI 5257 ATTTAAAT ATTT^AAAT Tth111I 2060 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1463 CCAGCAGCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 90 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 41 enzymes) # ################################### AclI 214 AACGTT AA^CGTT 5529 AACGTT AcyI 4937 GACGCC G[GA]^CG[CT]C 4946 GACGTC Alw44I 1966 GTGCAC G^TGCAC 5383 GTGCAC AocI 4470 CCTGAGG CC^T[ACGT]AGG 4826 CCTAAGG ApaI 3324 GGGCCC GGGCC^C 3919 GGGCCC ApaLI 1966 GTGCAC G^TGCAC 5383 GTGCAC BalI 1301 TGGCCA TGG^CCA 4885 TGGCCA BbrPI 1315 CACGTG CAC^GTG 1342 CACGTG BciVI 3949 GGATAC GTATCC(6/5) 2651 GTATCC BclI 209 TGATCA T^GATCA 4323 TGATCA BsaHI 4937 GACGCC G[GA]^CG[CT]C 4946 GACGTC BsaWI 1562 TCCGGT [AT]^CCGG[AT] 4410 TCCGGT BsmI 1884 GCATTC GAATGC(1/-1) 3023 GCATTC Bsp1407I 2015 TGTACA T^GTACA 4727 TGTACA BspLU11I 4354 ACATGT A^CATGT 4908 ACATGT BspMI 830 ACCTGC ACCTGC(4/8) 3094 ACCTGC BsrGI 2015 TGTACA T^GTACA 4727 TGTACA BstEII 1083 GGTGACC G^GT[ACGT]ACC 5059 GGTGACC BstPI 1083 GGTGACC G^GT[ACGT]ACC 5059 GGTGACC BstXI 20 CCATCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 381 CCACCCCCTTGG Bsu36I 4470 CCTGAGG CC^T[ACGT]AGG 4826 CCTAAGG Eco81I 4470 CCTGAGG CC^T[ACGT]AGG 4826 CCTAAGG EcoO65I 1083 GGTGACC G^GT[ACGT]ACC 5059 GGTGACC FbaI 209 TGATCA T^GATCA 4323 TGATCA Hin1I 4937 GACGCC G[GA]^CG[CT]C 4946 GACGTC HindIII 780 AAGCTT A^AGCTT 2642 AAGCTT HpaI 2320 GTTAAC GTT^AAC 4503 GTTAAC MluNI 1301 TGGCCA TGG^CCA 4885 TGGCCA MscI 1301 TGGCCA TGG^CCA 4885 TGGCCA PaeR7I 1336 CTCGAG C^TCGAG 3936 CTCGAG PmaCI 1315 CACGTG CAC^GTG 1342 CACGTG PmlI 1315 CACGTG CAC^GTG 1342 CACGTG Psp1406I 214 AACGTT AA^CGTT 5529 AACGTT SgrAI 1174 CACCGGCG C[GA]^CCGG[CT]G 1789 CACCGGCG SmaI 398 CCCGGG CCC^GGG 1079 CCCGGG SspBI 2015 TGTACA T^GTACA 4727 TGTACA SspI 3420 AATATT AAT^ATT 5578 AATATT TfiI 4566 GATTC G^A[AT]TC 5668 GATTC XhoI 1336 CTCGAG C^TCGAG 3936 CTCGAG XmaCI 398 CCCGGG C^CCGGG 1079 CCCGGG XmaI 398 CCCGGG C^CCGGG 1079 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI Bst1107I BstAPI BstF5I BstNI BstUI BstYI BstZ17I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XhoII