##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
Acc65I AccIII AflII AgeI AscI
Asp718I BbeI BfrI BlpI Bpu1102I
BsaBI BseAI BsiWI BspDI BspEI
BssHII BssSI BstBI CelII ClaI
CpoI DrdI EcoRI EcoRV EcoT22I
FseI KasI KpnI KspI MamI
MroI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PinAI PmeI PvuI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SnaBI SpeI Sse8387I
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 4946 GACGTC GACGT^C
AhdI 4017 GACTCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3357 AGCGCT AGC^GCT
AseI 3417 ATTAAT AT^TAAT
AsnI 3417 ATTAAT AT^TAAT
Asp700I 90 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4017 GACTCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2060 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 70 TGCGCA TGC^GCA
AvrII 609 CCTAGG C^CTAGG
BamHI 1796 GGATCC G^GATCC
BbvCI 2512 CCTCAGC CCTCAGC(-5/-2)
BcgI 5390 GCATCCATCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 58 GCCGTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4255 AGATCT A^GATCT
BlnI 609 CCTAGG C^CTAGG
BsiEI 848 CGGCCG CG[GA][CT]^CG
BsmBI 2841 CGTCTC CGTCTC(1/5)
BspHI 1946 TCATGA T^CATGA
BsrDI 3119 CATTGC GCAATG(2/0)
DraI 5258 TTTAAA TTT^AAA
DsaI 4941 CCACGG C^C[GA][CT]GG
EagI 848 CGGCCG C^GGCCG
Eam1105I 4017 GACTCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 848 CGGCCG C^GGCCG
Eco47III 3357 AGCGCT AGC^GCT
Eco52I 848 CGGCCG C^GGCCG
EcoNI 3910 CCTTCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 70 TGCGCA TGC^GCA
MfeI 4362 CAATTG C^AATTG
MluI 5410 ACGCGT A^CGCGT
MunI 4362 CAATTG C^AATTG
PacI 3389 TTAATTAA TTAAT^TAA
PflFI 2060 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1086 GACCCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3417 ATTAAT AT^TAAT
RcaI 1946 TCATGA T^CATGA
ScaI 5564 AGTACT AGT^ACT
SexAI 4489 ACCTGGT A^CC[AT]GGT
SphI 1005 GCATGC GCATG^C
SwaI 5257 ATTTAAAT ATTT^AAAT
Tth111I 2060 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1463 CCAGCAGCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 90 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AclI 214 AACGTT AA^CGTT
5529 AACGTT
AcyI 4937 GACGCC G[GA]^CG[CT]C
4946 GACGTC
Alw44I 1966 GTGCAC G^TGCAC
5383 GTGCAC
AocI 4470 CCTGAGG CC^T[ACGT]AGG
4826 CCTAAGG
ApaI 3324 GGGCCC GGGCC^C
3919 GGGCCC
ApaLI 1966 GTGCAC G^TGCAC
5383 GTGCAC
BalI 1301 TGGCCA TGG^CCA
4885 TGGCCA
BbrPI 1315 CACGTG CAC^GTG
1342 CACGTG
BciVI 3949 GGATAC GTATCC(6/5)
2651 GTATCC
BclI 209 TGATCA T^GATCA
4323 TGATCA
BsaHI 4937 GACGCC G[GA]^CG[CT]C
4946 GACGTC
BsaWI 1562 TCCGGT [AT]^CCGG[AT]
4410 TCCGGT
BsmI 1884 GCATTC GAATGC(1/-1)
3023 GCATTC
Bsp1407I 2015 TGTACA T^GTACA
4727 TGTACA
BspLU11I 4354 ACATGT A^CATGT
4908 ACATGT
BspMI 830 ACCTGC ACCTGC(4/8)
3094 ACCTGC
BsrGI 2015 TGTACA T^GTACA
4727 TGTACA
BstEII 1083 GGTGACC G^GT[ACGT]ACC
5059 GGTGACC
BstPI 1083 GGTGACC G^GT[ACGT]ACC
5059 GGTGACC
BstXI 20 CCATCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
381 CCACCCCCTTGG
Bsu36I 4470 CCTGAGG CC^T[ACGT]AGG
4826 CCTAAGG
Eco81I 4470 CCTGAGG CC^T[ACGT]AGG
4826 CCTAAGG
EcoO65I 1083 GGTGACC G^GT[ACGT]ACC
5059 GGTGACC
FbaI 209 TGATCA T^GATCA
4323 TGATCA
Hin1I 4937 GACGCC G[GA]^CG[CT]C
4946 GACGTC
HindIII 780 AAGCTT A^AGCTT
2642 AAGCTT
HpaI 2320 GTTAAC GTT^AAC
4503 GTTAAC
MluNI 1301 TGGCCA TGG^CCA
4885 TGGCCA
MscI 1301 TGGCCA TGG^CCA
4885 TGGCCA
PaeR7I 1336 CTCGAG C^TCGAG
3936 CTCGAG
PmaCI 1315 CACGTG CAC^GTG
1342 CACGTG
PmlI 1315 CACGTG CAC^GTG
1342 CACGTG
Psp1406I 214 AACGTT AA^CGTT
5529 AACGTT
SgrAI 1174 CACCGGCG C[GA]^CCGG[CT]G
1789 CACCGGCG
SmaI 398 CCCGGG CCC^GGG
1079 CCCGGG
SspBI 2015 TGTACA T^GTACA
4727 TGTACA
SspI 3420 AATATT AAT^ATT
5578 AATATT
TfiI 4566 GATTC G^A[AT]TC
5668 GATTC
XhoI 1336 CTCGAG C^TCGAG
3936 CTCGAG
XmaCI 398 CCCGGG C^CCGGG
1079 CCCGGG
XmaI 398 CCCGGG C^CCGGG
1079 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssKI Bst1107I BstAPI
BstF5I BstNI BstUI BstYI BstZ17I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XhoII