##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccII AccIII AclI AflIII
AgeI Alw44I AocI ApaLI AscI
AviII AvrII BbrPI BbvCI BcgI
BclI BlnI BlpI Bpu1102I BsaAI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI BstAPI BstBI
BstUI Bsu36I CelII ClaI CpoI
EagI EclXI Eco52I Eco81I FbaI
FseI FspI HindIII KspI MluI
MroI MvnI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
Psp1406I RcaI RsrII SacII SexAI
SfiI SfuI SgrAI SmaI SnaBI
SspBI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 115 GGTACC G^GTACC
AflII 931 CTTAAG C^TTAAG
AhdI 1270 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 4040 GGGCCC GGGCC^C
Asp718I 115 GGTACC G^GTACC
AspEI 1270 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3967 GGATCC G^GATCC
BfrI 931 CTTAAG C^TTAAG
BglI 2378 GCCCACTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI 895 CGATCG CG[GA][CT]^CG
BsmBI 3208 GAGACG CGTCTC(1/5)
BsrFI 3317 GCCGGC [GA]^CCGG[CT]
Cfr10I 3317 GCCGGC [GA]^CCGG[CT]
Eam1105I 1270 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 3687 GATATC GAT^ATC
FauI 525 GCGGG CCCGC(4/6)
HpaI 3163 GTTAAC GTT^AAC
KpnI 115 GGTACC GGTAC^C
MspA1I 155 CAGCTG C[AC]G^C[GT]G
NaeI 3317 GCCGGC GCC^GGC
NgoMIV 3317 GCCGGC G^CCGGC
NheI 2338 GCTAGC G^CTAGC
PshAI 1267 GACGACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 895 CGATCG CGAT^CG
PvuII 155 CAGCTG CAG^CTG
SalI 2393 GTCGAC G^TCGAC
SbfI 4977 CCTGCAGG CCTGCA^GG
ScaI 4357 AGTACT AGT^ACT
Sse8387I 4977 CCTGCAGG CCTGCA^GG
StuI 462 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 45 enzymes) #
###################################
Aor51HI 482 AGCGCT AGC^GCT
662 AGCGCT
AseI 2724 ATTAAT AT^TAAT
4809 ATTAAT
AsnI 2724 ATTAAT AT^TAAT
4809 ATTAAT
Asp700I 614 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3131 GAAGAGATTC
AspI 2289 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2421 GACTGGGTC
AvaI 2620 CTCGAG C^[CT]CG[GA]G
3515 CTCGAG
BbeI 2665 GGCGCC GGCGC^C
2840 GGCGCC
BciVI 1038 GGATAC GTATCC(6/5)
20 GTATCC
BcnI 3055 CCGGG CC^[GC]GG
4395 CCGGG
BsaBI 4147 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5238 GATATACATC
BsgI 3866 CTGCAC GTGCAG(16/14)
4533 GTGCAG
BsoBI 2620 CTCGAG C^[CT]CG[GA]G
3515 CTCGAG
BsrDI 306 CATTGC GCAATG(2/0)
3266 GCAATG
Bst1107I 5183 GTATAC GTA^TAC
5444 GTATAC
BstEII 4173 GGTTACC G^GT[ACGT]ACC
4542 GGTGACC
BstPI 4173 GGTTACC G^GT[ACGT]ACC
4542 GGTGACC
BstZ17I 5183 GTATAC GTA^TAC
5444 GTATAC
DraIII 1053 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1229 CACCAAGTG
DrdI 3115 GACTCTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4679 GACAAAAATGTC
Eco47III 482 AGCGCT AGC^GCT
662 AGCGCT
EcoNI 1542 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2816 CCTGGTATAGG
EcoO65I 4173 GGTTACC G^GT[ACGT]ACC
4542 GGTGACC
EcoRI 3840 GAATTC G^AATTC
4371 GAATTC
EcoT22I 2202 ATGCAT ATGCA^T
2463 ATGCAT
HgaI 3210 GACGC GACGC(5/10)
4233 GCGTC
KasI 2665 GGCGCC G^GCGCC
2840 GGCGCC
MaeII 2132 ACGT A^CGT
3248 ACGT
MamI 4147 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5238 GATATACATC
MfeI 1145 CAATTG C^AATTG
3853 CAATTG
MunI 1145 CAATTG C^AATTG
3853 CAATTG
NarI 2665 GGCGCC GG^CGCC
2840 GGCGCC
NciI 3055 CCGGG CC^[GC]GG
4395 CCGGG
NdeI 2998 CATATG CA^TATG
3991 CATATG
NsiI 2202 ATGCAT ATGCA^T
2463 ATGCAT
PaeR7I 2620 CTCGAG C^TCGAG
3515 CTCGAG
PflFI 2289 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2421 GACTGGGTC
PpuMI 2959 AGGACCT [GA]G^G[AT]CC[CT]
3789 GGGACCT
PshBI 2724 ATTAAT AT^TAAT
4809 ATTAAT
SfoI 2665 GGCGCC GGC^GCC
2840 GGCGCC
SspI 1474 AATATT AAT^ATT
4066 AATATT
SwaI 4461 ATTTAAAT ATTT^AAAT
5094 ATTTAAAT
Tth111I 2289 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2421 GACTGGGTC
XcmI 1245 CCAGTCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1662 CCATTTAATGACTGG
XhoI 2620 CTCGAG C^TCGAG
3515 CTCGAG
XmnI 614 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3131 GAAGAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BalI BanI BanII BbsI
BbvI BfaI BglII BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SpeI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII