################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AatII Acc65I AclI AflII AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI BciVI BfrI BsgI BspDI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI Eam1105I Eco47III Eco81I EcoNI EcoO65I FseI KpnI KspI MluI NaeI NcoI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PmeI PshAI PshBI Psp1406I RsrII SacII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI Sse8387I StuI SwaI XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccIII 137 TCCGGA T^CCGGA AgeI 5488 ACCGGT A^CCGGT AlwNI 5656 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 115 GGGCCC GGGCC^C AspI 4576 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 6036 TGCGCA TGC^GCA AvrII 2449 CCTAGG C^CTAGG BamHI 247 GGATCC G^GATCC BbeI 5782 GGCGCC GGCGC^C BbrPI 509 CACGTG CAC^GTG BclI 816 TGATCA T^GATCA BglII 6065 AGATCT A^GATCT BlnI 2449 CCTAGG C^CTAGG BseAI 137 TCCGGA T^CCGGA BsiWI 225 CGTACG C^GTACG BspEI 137 TCCGGA T^CCGGA BspLU11I 6031 ACATGT A^CATGT BssHII 345 GCGCGC G^CGCGC BstAPI 5655 GCAGCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 378 CCACCGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 3652 GACCAAATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 408 CCGTGG C^C[GA][CT]GG EagI 3854 CGGCCG C^GGCCG EclXI 3854 CGGCCG C^GGCCG Eco52I 3854 CGGCCG C^GGCCG EcoRV 794 GATATC GAT^ATC EcoT22I 3116 ATGCAT ATGCA^T FbaI 816 TGATCA T^GATCA FspI 6036 TGCGCA TGC^GCA HindIII 3453 AAGCTT A^AGCTT HpaI 566 GTTAAC GTT^AAC KasI 5782 GGCGCC G^GCGCC MfeI 304 CAATTG C^AATTG MroI 137 TCCGGA T^CCGGA MunI 304 CAATTG C^AATTG NarI 5782 GGCGCC GG^CGCC NheI 3816 GCTAGC G^CTAGC NsiI 3116 ATGCAT ATGCA^T PflFI 4576 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 5488 ACCGGT A^CCGGT PmaCI 509 CACGTG CAC^GTG PmlI 509 CACGTG CAC^GTG PvuI 2967 CGATCG CGAT^CG SexAI 2159 ACCAGGT A^CC[AT]GGT SfoI 5782 GGCGCC GGC^GCC SmaI 251 CCCGGG CCC^GGG SphI 2565 GCATGC GCATG^C Tth111I 4576 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 5002 TCTAGA T^CTAGA XmaCI 251 CCCGGG C^CCGGG XmaI 251 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcyI 7 GGCGTC G[GA]^CG[CT]C 5782 GGCGCC AflIII 4435 ACGTGT A^C[GA][CT]GT 6031 ACATGT BalI 1561 TGGCCA TGG^CCA 5079 TGGCCA BglI 33 GCCATCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 376 GCCCACCGGGC BsaAI 509 CACGTG [CT]AC^GT[GA] 662 CACGTA BsaHI 7 GGCGTC G[GA]^CG[CT]C 5782 GGCGCC BsaI 4186 GAGACC GGTCTC(1/5) 5600 GGTCTC BsiEI 2967 CGATCG CG[GA][CT]^CG 3854 CGGCCG BsmBI 3056 CGTCTC CGTCTC(1/5) 1632 GAGACG Bsp1407I 1278 TGTACA T^GTACA 1996 TGTACA BsrDI 400 CATTGC GCAATG(2/0) 2177 CATTGC BsrGI 1278 TGTACA T^GTACA 1996 TGTACA BssSI 1457 CACGAG CACGAG(-5/-1) 125 CTCGTG DraIII 1962 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG 2753 CACCCAGTG Hin1I 7 GGCGTC G[GA]^CG[CT]C 5782 GGCGCC MluNI 1561 TGGCCA TGG^CCA 5079 TGGCCA MscI 1561 TGGCCA TGG^CCA 5079 TGGCCA PpuMI 5137 GGGTCCT [GA]G^G[AT]CC[CT] 5156 AGGTCCT PvuII 242 CAGCTG CAG^CTG 326 CAGCTG SpeI 4252 ACTAGT A^CTAGT 5902 ACTAGT SspBI 1278 TGTACA T^GTACA 1996 TGTACA XcmI 468 CCACGAAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3654 CCAAATCGTCTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI