##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AclI AflII AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI BciVI
BfrI BsgI BspDI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI Eam1105I Eco47III Eco81I EcoNI
EcoO65I FseI KpnI KspI MluI
NaeI NcoI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PmeI
PshAI PshBI Psp1406I RsrII SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI Sse8387I StuI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccIII 137 TCCGGA T^CCGGA
AgeI 5488 ACCGGT A^CCGGT
AlwNI 5656 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 115 GGGCCC GGGCC^C
AspI 4576 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 6036 TGCGCA TGC^GCA
AvrII 2449 CCTAGG C^CTAGG
BamHI 247 GGATCC G^GATCC
BbeI 5782 GGCGCC GGCGC^C
BbrPI 509 CACGTG CAC^GTG
BclI 816 TGATCA T^GATCA
BglII 6065 AGATCT A^GATCT
BlnI 2449 CCTAGG C^CTAGG
BseAI 137 TCCGGA T^CCGGA
BsiWI 225 CGTACG C^GTACG
BspEI 137 TCCGGA T^CCGGA
BspLU11I 6031 ACATGT A^CATGT
BssHII 345 GCGCGC G^CGCGC
BstAPI 5655 GCAGCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 378 CCACCGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 3652 GACCAAATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 408 CCGTGG C^C[GA][CT]GG
EagI 3854 CGGCCG C^GGCCG
EclXI 3854 CGGCCG C^GGCCG
Eco52I 3854 CGGCCG C^GGCCG
EcoRV 794 GATATC GAT^ATC
EcoT22I 3116 ATGCAT ATGCA^T
FbaI 816 TGATCA T^GATCA
FspI 6036 TGCGCA TGC^GCA
HindIII 3453 AAGCTT A^AGCTT
HpaI 566 GTTAAC GTT^AAC
KasI 5782 GGCGCC G^GCGCC
MfeI 304 CAATTG C^AATTG
MroI 137 TCCGGA T^CCGGA
MunI 304 CAATTG C^AATTG
NarI 5782 GGCGCC GG^CGCC
NheI 3816 GCTAGC G^CTAGC
NsiI 3116 ATGCAT ATGCA^T
PflFI 4576 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 5488 ACCGGT A^CCGGT
PmaCI 509 CACGTG CAC^GTG
PmlI 509 CACGTG CAC^GTG
PvuI 2967 CGATCG CGAT^CG
SexAI 2159 ACCAGGT A^CC[AT]GGT
SfoI 5782 GGCGCC GGC^GCC
SmaI 251 CCCGGG CCC^GGG
SphI 2565 GCATGC GCATG^C
Tth111I 4576 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5002 TCTAGA T^CTAGA
XmaCI 251 CCCGGG C^CCGGG
XmaI 251 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 7 GGCGTC G[GA]^CG[CT]C
5782 GGCGCC
AflIII 4435 ACGTGT A^C[GA][CT]GT
6031 ACATGT
BalI 1561 TGGCCA TGG^CCA
5079 TGGCCA
BglI 33 GCCATCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
376 GCCCACCGGGC
BsaAI 509 CACGTG [CT]AC^GT[GA]
662 CACGTA
BsaHI 7 GGCGTC G[GA]^CG[CT]C
5782 GGCGCC
BsaI 4186 GAGACC GGTCTC(1/5)
5600 GGTCTC
BsiEI 2967 CGATCG CG[GA][CT]^CG
3854 CGGCCG
BsmBI 3056 CGTCTC CGTCTC(1/5)
1632 GAGACG
Bsp1407I 1278 TGTACA T^GTACA
1996 TGTACA
BsrDI 400 CATTGC GCAATG(2/0)
2177 CATTGC
BsrGI 1278 TGTACA T^GTACA
1996 TGTACA
BssSI 1457 CACGAG CACGAG(-5/-1)
125 CTCGTG
DraIII 1962 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
2753 CACCCAGTG
Hin1I 7 GGCGTC G[GA]^CG[CT]C
5782 GGCGCC
MluNI 1561 TGGCCA TGG^CCA
5079 TGGCCA
MscI 1561 TGGCCA TGG^CCA
5079 TGGCCA
PpuMI 5137 GGGTCCT [GA]G^G[AT]CC[CT]
5156 AGGTCCT
PvuII 242 CAGCTG CAG^CTG
326 CAGCTG
SpeI 4252 ACTAGT A^CTAGT
5902 ACTAGT
SspBI 1278 TGTACA T^GTACA
1996 TGTACA
XcmI 468 CCACGAAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3654 CCAAATCGTCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApoI Asp700I AspHI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcgI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaBI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI RcaI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI