################################## # NO CUT ENZYMES ( 52 enzymes) # ################################## AgeI Alw44I ApaLI AscI AspI AviII BbeI BbrPI BcgI BglII BsaAI BsiWI BsmBI BsmI Bsp1407I BspDI BsrGI BssHII Bst1107I BstZ17I ClaI CpoI DrdI EcoT22I FspI HpaI KasI MfeI MluI MunI NarI NdeI NotI NruI NsiI PacI PflFI PinAI PmaCI PmlI PvuI RsrII SbfI SfiI SfoI SgrAI SnaBI SpeI Sse8387I SspBI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 1212 GACGTC GACGT^C Acc65I 589 GGTACC G^GTACC AccIII 2720 TCCGGA T^CCGGA AclI 5355 AACGTT AA^CGTT AcsI 2309 GAATTC [GA]^AATT[CT] AflIII 775 ACATGT A^C[GA][CT]GT AocI 3100 CCTCAGG CC^T[ACGT]AGG Aor51HI 222 AGCGCT AGC^GCT ApoI 2309 GAATTC [GA]^AATT[CT] AseI 4354 ATTAAT AT^TAAT AsnI 4354 ATTAAT AT^TAAT Asp718I 589 GGTACC G^GTACC BclI 3653 TGATCA T^GATCA BlpI 58 GCTAAGC GC^T[ACGT]AGC Bpu1102I 58 GCTAAGC GC^T[ACGT]AGC BsaBI 422 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2720 TCCGGA [AT]^CCGG[AT] BseAI 2720 TCCGGA T^CCGGA BspEI 2720 TCCGGA T^CCGGA BspHI 3656 TCATGA T^CATGA BspLU11I 775 ACATGT A^CATGT BsrBI 2629 CCGCTC CCGCTC(-3/-3) BssSI 2090 CTCGTG CACGAG(-5/-1) BstAPI 1134 GCACCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 567 TTCGAA TT^CGAA Bsu36I 3100 CCTCAGG CC^T[ACGT]AGG CelII 58 GCTAAGC GC^T[ACGT]AGC Eco47III 222 AGCGCT AGC^GCT Eco81I 3100 CCTCAGG CC^T[ACGT]AGG EcoRI 2309 GAATTC G^AATTC EcoRV 2205 GATATC GAT^ATC FbaI 3653 TGATCA T^GATCA FseI 65 GGCCGGCC GGCCGG^CC KpnI 589 GGTACC GGTAC^C MamI 422 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2720 TCCGGA T^CCGGA NcoI 931 CCATGG C^CATGG NheI 2556 GCTAGC G^CTAGC NspV 567 TTCGAA TT^CGAA PaeR7I 944 CTCGAG C^TCGAG PmeI 5358 GTTTAAAC GTTT^AAAC PshBI 4354 ATTAAT AT^TAAT Psp1406I 5355 AACGTT AA^CGTT RcaI 3656 TCATGA T^CATGA SalI 3064 GTCGAC G^TCGAC SexAI 1411 ACCAGGT A^CC[AT]GGT SfuI 567 TTCGAA TT^CGAA SmaI 212 CCCGGG CCC^GGG XhoI 944 CTCGAG C^TCGAG XmaCI 212 CCCGGG C^CCGGG XmaI 212 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 45 enzymes) # ################################### AcyI 37 GGCGTC G[GA]^CG[CT]C 1212 GACGTC AflII 2636 CTTAAG C^TTAAG 4460 CTTAAG AhdI 2695 GACTCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3027 GACTCGGAGTC AlwNI 2561 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG 2847 CAGGCTCTG ApaI 209 GGGCCC GGGCC^C 215 GGGCCC Asp700I 1547 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3549 GAAAGTATTC AspEI 2695 GACTCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3027 GACTCGGAGTC AvrII 4892 CCTAGG C^CTAGG 5028 CCTAGG BalI 1894 TGGCCA TGG^CCA 3942 TGGCCA BbsI 2433 GAAGAC GAAGAC(2/6) 5138 GTCTTC BciVI 84 GGATAC GTATCC(6/5) 4749 GTATCC BfrI 2636 CTTAAG C^TTAAG 4460 CTTAAG BlnI 4892 CCTAGG C^CTAGG 5028 CCTAGG BpuAI 2433 GAAGAC GAAGAC(2/6) 5138 GTCTTC BsaHI 37 GGCGTC G[GA]^CG[CT]C 1212 GACGTC BsiEI 64 CGGCCG CG[GA][CT]^CG 3738 CGGCCG BstEII 1274 GGTCACC G^GT[ACGT]ACC 3853 GGTAACC BstPI 1274 GGTCACC G^GT[ACGT]ACC 3853 GGTAACC CfoI 56 GCGC GCG^C 223 GCGC DraI 5347 TTTAAA TTT^AAA 5359 TTTAAA DraIII 1410 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG 3184 CACAGAGTG EagI 64 CGGCCG C^GGCCG 3738 CGGCCG Eam1105I 2695 GACTCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3027 GACTCGGAGTC EclXI 64 CGGCCG C^GGCCG 3738 CGGCCG Eco52I 64 CGGCCG C^GGCCG 3738 CGGCCG EcoNI 989 CCTCGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4609 CCTAGAATAGG EcoO65I 1274 GGTCACC G^GT[ACGT]ACC 3853 GGTAACC HaeII 55 GGCGCT [GA]GCGC^[CT] 222 AGCGCT HgaI 38 GCGTC GACGC(5/10) 3601 GCGTC HhaI 56 GCGC GCG^C 223 GCGC Hin1I 37 GGCGTC G[GA]^CG[CT]C 1212 GACGTC HinP1I 56 GCGC G^CGC 223 GCGC KspI 11 CCGCGG CCGC^GG 302 CCGCGG MluNI 1894 TGGCCA TGG^CCA 3942 TGGCCA MscI 1894 TGGCCA TGG^CCA 3942 TGGCCA NaeI 66 GCCGGC GCC^GGC 243 GCCGGC NgoMIV 66 GCCGGC G^CCGGC 243 GCCGGC SacI 786 GAGCTC GAGCT^C 2366 GAGCTC SacII 11 CCGCGG CCGC^GG 302 CCGCGG SapI 2682 GAAGAGC GCTCTTC(1/4) 5281 GAAGAGC ScaI 511 AGTACT AGT^ACT 736 AGTACT SphI 984 GCATGC GCATG^C 4870 GCATGC StuI 1714 AGGCCT AGG^CCT 4774 AGGCCT XbaI 3608 TCTAGA T^CTAGA 4953 TCTAGA XmnI 1547 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3549 GAAAGTATTC ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AccI AccII AciI AfaI AluI AlwI AspHI AvaI AvaII BamHI BanI BanII BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII