##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AgeI Alw44I ApaLI AscI AspI
AviII BbeI BbrPI BcgI BglII
BsaAI BsiWI BsmBI BsmI Bsp1407I
BspDI BsrGI BssHII Bst1107I BstZ17I
ClaI CpoI DrdI EcoT22I FspI
HpaI KasI MfeI MluI MunI
NarI NdeI NotI NruI NsiI
PacI PflFI PinAI PmaCI PmlI
PvuI RsrII SbfI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 1212 GACGTC GACGT^C
Acc65I 589 GGTACC G^GTACC
AccIII 2720 TCCGGA T^CCGGA
AclI 5355 AACGTT AA^CGTT
AcsI 2309 GAATTC [GA]^AATT[CT]
AflIII 775 ACATGT A^C[GA][CT]GT
AocI 3100 CCTCAGG CC^T[ACGT]AGG
Aor51HI 222 AGCGCT AGC^GCT
ApoI 2309 GAATTC [GA]^AATT[CT]
AseI 4354 ATTAAT AT^TAAT
AsnI 4354 ATTAAT AT^TAAT
Asp718I 589 GGTACC G^GTACC
BclI 3653 TGATCA T^GATCA
BlpI 58 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 58 GCTAAGC GC^T[ACGT]AGC
BsaBI 422 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2720 TCCGGA [AT]^CCGG[AT]
BseAI 2720 TCCGGA T^CCGGA
BspEI 2720 TCCGGA T^CCGGA
BspHI 3656 TCATGA T^CATGA
BspLU11I 775 ACATGT A^CATGT
BsrBI 2629 CCGCTC CCGCTC(-3/-3)
BssSI 2090 CTCGTG CACGAG(-5/-1)
BstAPI 1134 GCACCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 567 TTCGAA TT^CGAA
Bsu36I 3100 CCTCAGG CC^T[ACGT]AGG
CelII 58 GCTAAGC GC^T[ACGT]AGC
Eco47III 222 AGCGCT AGC^GCT
Eco81I 3100 CCTCAGG CC^T[ACGT]AGG
EcoRI 2309 GAATTC G^AATTC
EcoRV 2205 GATATC GAT^ATC
FbaI 3653 TGATCA T^GATCA
FseI 65 GGCCGGCC GGCCGG^CC
KpnI 589 GGTACC GGTAC^C
MamI 422 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2720 TCCGGA T^CCGGA
NcoI 931 CCATGG C^CATGG
NheI 2556 GCTAGC G^CTAGC
NspV 567 TTCGAA TT^CGAA
PaeR7I 944 CTCGAG C^TCGAG
PmeI 5358 GTTTAAAC GTTT^AAAC
PshBI 4354 ATTAAT AT^TAAT
Psp1406I 5355 AACGTT AA^CGTT
RcaI 3656 TCATGA T^CATGA
SalI 3064 GTCGAC G^TCGAC
SexAI 1411 ACCAGGT A^CC[AT]GGT
SfuI 567 TTCGAA TT^CGAA
SmaI 212 CCCGGG CCC^GGG
XhoI 944 CTCGAG C^TCGAG
XmaCI 212 CCCGGG C^CCGGG
XmaI 212 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 45 enzymes) #
###################################
AcyI 37 GGCGTC G[GA]^CG[CT]C
1212 GACGTC
AflII 2636 CTTAAG C^TTAAG
4460 CTTAAG
AhdI 2695 GACTCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3027 GACTCGGAGTC
AlwNI 2561 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
2847 CAGGCTCTG
ApaI 209 GGGCCC GGGCC^C
215 GGGCCC
Asp700I 1547 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3549 GAAAGTATTC
AspEI 2695 GACTCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3027 GACTCGGAGTC
AvrII 4892 CCTAGG C^CTAGG
5028 CCTAGG
BalI 1894 TGGCCA TGG^CCA
3942 TGGCCA
BbsI 2433 GAAGAC GAAGAC(2/6)
5138 GTCTTC
BciVI 84 GGATAC GTATCC(6/5)
4749 GTATCC
BfrI 2636 CTTAAG C^TTAAG
4460 CTTAAG
BlnI 4892 CCTAGG C^CTAGG
5028 CCTAGG
BpuAI 2433 GAAGAC GAAGAC(2/6)
5138 GTCTTC
BsaHI 37 GGCGTC G[GA]^CG[CT]C
1212 GACGTC
BsiEI 64 CGGCCG CG[GA][CT]^CG
3738 CGGCCG
BstEII 1274 GGTCACC G^GT[ACGT]ACC
3853 GGTAACC
BstPI 1274 GGTCACC G^GT[ACGT]ACC
3853 GGTAACC
CfoI 56 GCGC GCG^C
223 GCGC
DraI 5347 TTTAAA TTT^AAA
5359 TTTAAA
DraIII 1410 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3184 CACAGAGTG
EagI 64 CGGCCG C^GGCCG
3738 CGGCCG
Eam1105I 2695 GACTCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3027 GACTCGGAGTC
EclXI 64 CGGCCG C^GGCCG
3738 CGGCCG
Eco52I 64 CGGCCG C^GGCCG
3738 CGGCCG
EcoNI 989 CCTCGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4609 CCTAGAATAGG
EcoO65I 1274 GGTCACC G^GT[ACGT]ACC
3853 GGTAACC
HaeII 55 GGCGCT [GA]GCGC^[CT]
222 AGCGCT
HgaI 38 GCGTC GACGC(5/10)
3601 GCGTC
HhaI 56 GCGC GCG^C
223 GCGC
Hin1I 37 GGCGTC G[GA]^CG[CT]C
1212 GACGTC
HinP1I 56 GCGC G^CGC
223 GCGC
KspI 11 CCGCGG CCGC^GG
302 CCGCGG
MluNI 1894 TGGCCA TGG^CCA
3942 TGGCCA
MscI 1894 TGGCCA TGG^CCA
3942 TGGCCA
NaeI 66 GCCGGC GCC^GGC
243 GCCGGC
NgoMIV 66 GCCGGC G^CCGGC
243 GCCGGC
SacI 786 GAGCTC GAGCT^C
2366 GAGCTC
SacII 11 CCGCGG CCGC^GG
302 CCGCGG
SapI 2682 GAAGAGC GCTCTTC(1/4)
5281 GAAGAGC
ScaI 511 AGTACT AGT^ACT
736 AGTACT
SphI 984 GCATGC GCATG^C
4870 GCATGC
StuI 1714 AGGCCT AGG^CCT
4774 AGGCCT
XbaI 3608 TCTAGA T^CTAGA
4953 TCTAGA
XmnI 1547 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3549 GAAAGTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AspHI AvaI AvaII BamHI
BanI BanII BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PshAI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII