################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII Acc65I AccIII AgeI Aor51HI ApaI AscI Asp718I AvaI AviII AvrII BanI BanII BbeI BbvCI BcgI BglII BlnI BsaWI BseAI BsiWI BsoBI BspEI BssHII BssSI Bst1107I BstEII BstPI BstZ17I CpoI Eco47III EcoO65I FseI FspI HpaI KasI KpnI MfeI MluI MroI MunI NaeI NarI NgoMIV NotI NruI PacI PaeR7I PinAI PmeI PshAI PvuI RsrII SacI SalI SbfI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I StuI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 3702 GTCTAC GT^[AC][GT]AC AccII 1501 CGCG CG^CG AclI 3328 AACGTT AA^CGTT AhdI 5025 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 3662 CCTCAGG CC^T[ACGT]AGG AspEI 5025 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 5139 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1666 GGATCC G^GATCC BbrPI 2929 CACGTG CAC^GTG BglI 1150 GCCTCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1451 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1451 GCTCAGC GC^T[ACGT]AGC BsiEI 2109 CGGCCG CG[GA][CT]^CG BsmBI 5023 GAGACG CGTCTC(1/5) Bsp1407I 4164 TGTACA T^GTACA BsrBI 2152 CCGCTC CCGCTC(-3/-3) BsrFI 150 GCCGGT [GA]^CCGG[CT] BsrGI 4164 TGTACA T^GTACA BstUI 1501 CGCG CG^CG Bsu36I 3662 CCTCAGG CC^T[ACGT]AGG CelII 1451 GCTCAGC GC^T[ACGT]AGC Cfr10I 150 GCCGGT [GA]^CCGG[CT] DraII 5137 AGGACCC [GA]G^G[ACGT]CC[CT] DraIII 2908 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 2109 CGGCCG C^GGCCG Eam1105I 5025 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2109 CGGCCG C^GGCCG Eco52I 2109 CGGCCG C^GGCCG Eco81I 3662 CCTCAGG CC^T[ACGT]AGG EcoNI 98 CCTGACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 5137 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRV 3989 GATATC GAT^ATC EcoT14I 4801 CCATGG C^C[AT][AT]GG EcoT22I 4981 ATGCAT ATGCA^T HaeII 112 GGCGCT [GA]GCGC^[CT] KspI 1500 CCGCGG CCGC^GG MvnI 1501 CGCG CG^CG NcoI 4801 CCATGG C^CATGG NdeI 2220 CATATG CA^TATG NheI 1658 GCTAGC G^CTAGC NsiI 4981 ATGCAT ATGCA^T PflFI 5139 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2929 CACGTG CAC^GTG PmlI 2929 CACGTG CAC^GTG PpuMI 5137 AGGACCC [GA]G^G[AT]CC[CT] Psp1406I 3328 AACGTT AA^CGTT PstI 2211 CTGCAG CTGCA^G SacII 1500 CCGCGG CCGC^GG ScaI 5236 AGTACT AGT^ACT SphI 4021 GCATGC GCATG^C SspBI 4164 TGTACA T^GTACA StyI 4801 CCATGG C^C[AT][AT]GG Tth111I 5139 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcyI 227 GGCGTC G[GA]^CG[CT]C 5025 GACGCC AvaII 2437 GGTCC G^G[AT]CC 5138 GGACC BalI 4690 TGGCCA TGG^CCA 4804 TGGCCA BciVI 568 GGATAC GTATCC(6/5) 2745 GTATCC BpmI 2652 CTCCAG CTGGAG(16/14) 4680 CTGGAG BsaAI 974 CACGTA [CT]AC^GT[GA] 2929 CACGTG BsaHI 227 GGCGTC G[GA]^CG[CT]C 5025 GACGCC BspDI 1007 ATCGAT AT^CGAT 2051 ATCGAT BspHI 1315 TCATGA T^CATGA 4987 TCATGA BspLU11I 741 ACATGT A^CATGT 3815 ACATGT BsrDI 663 GCAATG GCAATG(2/0) 5119 GCAATG BstBI 477 TTCGAA TT^CGAA 1719 TTCGAA BstXI 1864 CCAGGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4220 CCAGTGCTTTGG ClaI 1007 ATCGAT AT^CGAT 2051 ATCGAT DrdI 2218 GACATATGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4061 GACTGTGCTGTC DsaI 1500 CCGCGG C^C[GA][CT]GG 4801 CCATGG EcoRI 410 GAATTC G^AATTC 1046 GAATTC Hin1I 227 GGCGTC G[GA]^CG[CT]C 5025 GACGCC HincII 2335 GTCAAC GT[CT]^[GA]AC 2620 GTCAAC HindII 2335 GTCAAC GT[CT]^[GA]AC 2620 GTCAAC HindIII 4533 AAGCTT A^AGCTT 4644 AAGCTT MluNI 4690 TGGCCA TGG^CCA 4804 TGGCCA MscI 4690 TGGCCA TGG^CCA 4804 TGGCCA NspV 477 TTCGAA TT^CGAA 1719 TTCGAA PflMI 1609 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3631 CCAGCTGTTGG RcaI 1315 TCATGA T^CATGA 4987 TCATGA SapI 1057 GAAGAGC GCTCTTC(1/4) 3263 GCTCTTC SfuI 477 TTCGAA TT^CGAA 1719 TTCGAA SwaI 4280 ATTTAAAT ATTT^AAAT 5221 ATTTAAAT Van91I 1609 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3631 CCAGCTGTTGG XbaI 2005 TCTAGA T^CTAGA 3873 TCTAGA XcmI 1437 CCATGATGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2064 CCATAAGTGCACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AciI AcsI AfaI AflII AflIII AluI Alw44I AlwI AlwNI ApaLI ApoI AseI AsnI Asp700I AspHI BbsI BbvI BclI BcnI BfaI BfrI BmyI BpuAI BsaBI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EaeI EarI EcoRII FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PshBI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI