##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AatII       Acc65I      AccIII      AgeI        Aor51HI     
ApaI        AscI        Asp718I     AvaI        AviII       
AvrII       BanI        BanII       BbeI        BbvCI       
BcgI        BglII       BlnI        BsaWI       BseAI       
BsiWI       BsoBI       BspEI       BssHII      BssSI       
Bst1107I    BstEII      BstPI       BstZ17I     CpoI        
Eco47III    EcoO65I     FseI        FspI        HpaI        
KasI        KpnI        MfeI        MluI        MroI        
MunI        NaeI        NarI        NgoMIV      NotI        
NruI        PacI        PaeR7I      PinAI       PmeI        
PshAI       PvuI        RsrII       SacI        SalI        
SbfI        SfiI        SfoI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    StuI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccI         3702  GTCTAC               GT^[AC][GT]AC
AccII        1501  CGCG                 CG^CG
AclI         3328  AACGTT               AA^CGTT
AhdI         5025  GACGCCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         3662  CCTCAGG              CC^T[ACGT]AGG
AspEI        5025  GACGCCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         5139  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1666  GGATCC               G^GATCC
BbrPI        2929  CACGTG               CAC^GTG
BglI         1150  GCCTCTCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         1451  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1451  GCTCAGC              GC^T[ACGT]AGC
BsiEI        2109  CGGCCG               CG[GA][CT]^CG
BsmBI        5023  GAGACG               CGTCTC(1/5)
Bsp1407I     4164  TGTACA               T^GTACA
BsrBI        2152  CCGCTC               CCGCTC(-3/-3)
BsrFI         150  GCCGGT               [GA]^CCGG[CT]
BsrGI        4164  TGTACA               T^GTACA
BstUI        1501  CGCG                 CG^CG
Bsu36I       3662  CCTCAGG              CC^T[ACGT]AGG
CelII        1451  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        150  GCCGGT               [GA]^CCGG[CT]
DraII        5137  AGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII       2908  CACCTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         2109  CGGCCG               C^GGCCG
Eam1105I     5025  GACGCCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2109  CGGCCG               C^GGCCG
Eco52I       2109  CGGCCG               C^GGCCG
Eco81I       3662  CCTCAGG              CC^T[ACGT]AGG
EcoNI          98  CCTGACAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     5137  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRV        3989  GATATC               GAT^ATC
EcoT14I      4801  CCATGG               C^C[AT][AT]GG
EcoT22I      4981  ATGCAT               ATGCA^T
HaeII         112  GGCGCT               [GA]GCGC^[CT]
KspI         1500  CCGCGG               CCGC^GG
MvnI         1501  CGCG                 CG^CG
NcoI         4801  CCATGG               C^CATGG
NdeI         2220  CATATG               CA^TATG
NheI         1658  GCTAGC               G^CTAGC
NsiI         4981  ATGCAT               ATGCA^T
PflFI        5139  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        2929  CACGTG               CAC^GTG
PmlI         2929  CACGTG               CAC^GTG
PpuMI        5137  AGGACCC              [GA]G^G[AT]CC[CT]
Psp1406I     3328  AACGTT               AA^CGTT
PstI         2211  CTGCAG               CTGCA^G
SacII        1500  CCGCGG               CCGC^GG
ScaI         5236  AGTACT               AGT^ACT
SphI         4021  GCATGC               GCATG^C
SspBI        4164  TGTACA               T^GTACA
StyI         4801  CCATGG               C^C[AT][AT]GG
Tth111I      5139  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AcyI          227  GGCGTC               G[GA]^CG[CT]C
             5025  GACGCC              
AvaII        2437  GGTCC                G^G[AT]CC
             5138  GGACC               
BalI         4690  TGGCCA               TGG^CCA
             4804  TGGCCA              
BciVI         568  GGATAC               GTATCC(6/5)
             2745  GTATCC              
BpmI         2652  CTCCAG               CTGGAG(16/14)
             4680  CTGGAG              
BsaAI         974  CACGTA               [CT]AC^GT[GA]
             2929  CACGTG              
BsaHI         227  GGCGTC               G[GA]^CG[CT]C
             5025  GACGCC              
BspDI        1007  ATCGAT               AT^CGAT
             2051  ATCGAT              
BspHI        1315  TCATGA               T^CATGA
             4987  TCATGA              
BspLU11I      741  ACATGT               A^CATGT
             3815  ACATGT              
BsrDI         663  GCAATG               GCAATG(2/0)
             5119  GCAATG              
BstBI         477  TTCGAA               TT^CGAA
             1719  TTCGAA              
BstXI        1864  CCAGGTTCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4220  CCAGTGCTTTGG        
ClaI         1007  ATCGAT               AT^CGAT
             2051  ATCGAT              
DrdI         2218  GACATATGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4061  GACTGTGCTGTC        
DsaI         1500  CCGCGG               C^C[GA][CT]GG
             4801  CCATGG              
EcoRI         410  GAATTC               G^AATTC
             1046  GAATTC              
Hin1I         227  GGCGTC               G[GA]^CG[CT]C
             5025  GACGCC              
HincII       2335  GTCAAC               GT[CT]^[GA]AC
             2620  GTCAAC              
HindII       2335  GTCAAC               GT[CT]^[GA]AC
             2620  GTCAAC              
HindIII      4533  AAGCTT               A^AGCTT
             4644  AAGCTT              
MluNI        4690  TGGCCA               TGG^CCA
             4804  TGGCCA              
MscI         4690  TGGCCA               TGG^CCA
             4804  TGGCCA              
NspV          477  TTCGAA               TT^CGAA
             1719  TTCGAA              
PflMI        1609  CCACAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3631  CCAGCTGTTGG         
RcaI         1315  TCATGA               T^CATGA
             4987  TCATGA              
SapI         1057  GAAGAGC              GCTCTTC(1/4)
             3263  GCTCTTC             
SfuI          477  TTCGAA               TT^CGAA
             1719  TTCGAA              
SwaI         4280  ATTTAAAT             ATTT^AAAT
             5221  ATTTAAAT            
Van91I       1609  CCACAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3631  CCAGCTGTTGG         
XbaI         2005  TCTAGA               T^CTAGA
             3873  TCTAGA              
XcmI         1437  CCATGATGGGTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2064  CCATAAGTGCACTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  106 enzymes) #
#########################################

AciI        AcsI        AfaI        AflII       AflIII      
AluI        Alw44I      AlwI        AlwNI       ApaLI       
ApoI        AseI        AsnI        Asp700I     AspHI       
BbsI        BbvI        BclI        BcnI        BfaI        
BfrI        BmyI        BpuAI       BsaBI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
BspMI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        EaeI        EarI        
EcoRII      FauI        FbaI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MamI        MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PshBI       PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SexAI       SfaNI       SfcI        SmlI        
SspI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
XmnI