##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AccIII AgeI Aor51HI
ApaI AscI Asp718I AvaI AviII
AvrII BanI BanII BbeI BbvCI
BcgI BglII BlnI BsaWI BseAI
BsiWI BsoBI BspEI BssHII BssSI
Bst1107I BstEII BstPI BstZ17I CpoI
Eco47III EcoO65I FseI FspI HpaI
KasI KpnI MfeI MluI MroI
MunI NaeI NarI NgoMIV NotI
NruI PacI PaeR7I PinAI PmeI
PshAI PvuI RsrII SacI SalI
SbfI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I StuI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 3702 GTCTAC GT^[AC][GT]AC
AccII 1501 CGCG CG^CG
AclI 3328 AACGTT AA^CGTT
AhdI 5025 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3662 CCTCAGG CC^T[ACGT]AGG
AspEI 5025 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5139 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1666 GGATCC G^GATCC
BbrPI 2929 CACGTG CAC^GTG
BglI 1150 GCCTCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1451 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1451 GCTCAGC GC^T[ACGT]AGC
BsiEI 2109 CGGCCG CG[GA][CT]^CG
BsmBI 5023 GAGACG CGTCTC(1/5)
Bsp1407I 4164 TGTACA T^GTACA
BsrBI 2152 CCGCTC CCGCTC(-3/-3)
BsrFI 150 GCCGGT [GA]^CCGG[CT]
BsrGI 4164 TGTACA T^GTACA
BstUI 1501 CGCG CG^CG
Bsu36I 3662 CCTCAGG CC^T[ACGT]AGG
CelII 1451 GCTCAGC GC^T[ACGT]AGC
Cfr10I 150 GCCGGT [GA]^CCGG[CT]
DraII 5137 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 2908 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2109 CGGCCG C^GGCCG
Eam1105I 5025 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2109 CGGCCG C^GGCCG
Eco52I 2109 CGGCCG C^GGCCG
Eco81I 3662 CCTCAGG CC^T[ACGT]AGG
EcoNI 98 CCTGACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 5137 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 3989 GATATC GAT^ATC
EcoT14I 4801 CCATGG C^C[AT][AT]GG
EcoT22I 4981 ATGCAT ATGCA^T
HaeII 112 GGCGCT [GA]GCGC^[CT]
KspI 1500 CCGCGG CCGC^GG
MvnI 1501 CGCG CG^CG
NcoI 4801 CCATGG C^CATGG
NdeI 2220 CATATG CA^TATG
NheI 1658 GCTAGC G^CTAGC
NsiI 4981 ATGCAT ATGCA^T
PflFI 5139 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2929 CACGTG CAC^GTG
PmlI 2929 CACGTG CAC^GTG
PpuMI 5137 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 3328 AACGTT AA^CGTT
PstI 2211 CTGCAG CTGCA^G
SacII 1500 CCGCGG CCGC^GG
ScaI 5236 AGTACT AGT^ACT
SphI 4021 GCATGC GCATG^C
SspBI 4164 TGTACA T^GTACA
StyI 4801 CCATGG C^C[AT][AT]GG
Tth111I 5139 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 227 GGCGTC G[GA]^CG[CT]C
5025 GACGCC
AvaII 2437 GGTCC G^G[AT]CC
5138 GGACC
BalI 4690 TGGCCA TGG^CCA
4804 TGGCCA
BciVI 568 GGATAC GTATCC(6/5)
2745 GTATCC
BpmI 2652 CTCCAG CTGGAG(16/14)
4680 CTGGAG
BsaAI 974 CACGTA [CT]AC^GT[GA]
2929 CACGTG
BsaHI 227 GGCGTC G[GA]^CG[CT]C
5025 GACGCC
BspDI 1007 ATCGAT AT^CGAT
2051 ATCGAT
BspHI 1315 TCATGA T^CATGA
4987 TCATGA
BspLU11I 741 ACATGT A^CATGT
3815 ACATGT
BsrDI 663 GCAATG GCAATG(2/0)
5119 GCAATG
BstBI 477 TTCGAA TT^CGAA
1719 TTCGAA
BstXI 1864 CCAGGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4220 CCAGTGCTTTGG
ClaI 1007 ATCGAT AT^CGAT
2051 ATCGAT
DrdI 2218 GACATATGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4061 GACTGTGCTGTC
DsaI 1500 CCGCGG C^C[GA][CT]GG
4801 CCATGG
EcoRI 410 GAATTC G^AATTC
1046 GAATTC
Hin1I 227 GGCGTC G[GA]^CG[CT]C
5025 GACGCC
HincII 2335 GTCAAC GT[CT]^[GA]AC
2620 GTCAAC
HindII 2335 GTCAAC GT[CT]^[GA]AC
2620 GTCAAC
HindIII 4533 AAGCTT A^AGCTT
4644 AAGCTT
MluNI 4690 TGGCCA TGG^CCA
4804 TGGCCA
MscI 4690 TGGCCA TGG^CCA
4804 TGGCCA
NspV 477 TTCGAA TT^CGAA
1719 TTCGAA
PflMI 1609 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3631 CCAGCTGTTGG
RcaI 1315 TCATGA T^CATGA
4987 TCATGA
SapI 1057 GAAGAGC GCTCTTC(1/4)
3263 GCTCTTC
SfuI 477 TTCGAA TT^CGAA
1719 TTCGAA
SwaI 4280 ATTTAAAT ATTT^AAAT
5221 ATTTAAAT
Van91I 1609 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3631 CCAGCTGTTGG
XbaI 2005 TCTAGA T^CTAGA
3873 TCTAGA
XcmI 1437 CCATGATGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2064 CCATAAGTGCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI Alw44I AlwI AlwNI ApaLI
ApoI AseI AsnI Asp700I AspHI
BbsI BbvI BclI BcnI BfaI
BfrI BmyI BpuAI BsaBI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI EaeI EarI
EcoRII FauI FbaI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PshBI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI