##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
AhdI AocI Aor51HI ApaI AscI
Asp718I AspEI AspI AviII AvrII
BbeI BbrPI BbvCI BclI BglI
BlnI BlpI Bpu1102I BsaHI BsiWI
BspDI BspHI BsrBI BssHII BstBI
BstEII BstPI Bsu36I CelII ClaI
CpoI DrdI Eam1105I Eco47III Eco81I
EcoO65I FbaI FseI FspI Hin1I
KasI KpnI KspI MfeI MluI
MunI NaeI NarI NcoI NgoMIV
NheI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI StuI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 447 TCCGGA T^CCGGA
AflII 3047 CTTAAG C^TTAAG
AseI 3320 ATTAAT AT^TAAT
AsnI 3320 ATTAAT AT^TAAT
BfrI 3047 CTTAAG C^TTAAG
BsaAI 693 CACGTA [CT]AC^GT[GA]
BsaBI 3553 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1256 GAGACC GGTCTC(1/5)
BsaWI 447 TCCGGA [AT]^CCGG[AT]
BseAI 447 TCCGGA T^CCGGA
BsgI 2619 GTGCAG GTGCAG(16/14)
BsiEI 68 CGGCCG CG[GA][CT]^CG
BsmI 2091 GCATTC GAATGC(1/-1)
Bsp1407I 2368 TGTACA T^GTACA
BspEI 447 TCCGGA T^CCGGA
BsrFI 1750 GCCGGT [GA]^CCGG[CT]
BsrGI 2368 TGTACA T^GTACA
BstAPI 4204 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 4807 CCATTTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1750 GCCGGT [GA]^CCGG[CT]
DraIII 4845 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 173 CCGTGG C^C[GA][CT]GG
EagI 68 CGGCCG C^GGCCG
EclXI 68 CGGCCG C^GGCCG
Eco52I 68 CGGCCG C^GGCCG
EcoRV 4510 GATATC GAT^ATC
HaeII 240 GGCGCT [GA]GCGC^[CT]
HgaI 1583 GCGTC GACGC(5/10)
HpaI 4739 GTTAAC GTT^AAC
MamI 3553 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 447 TCCGGA T^CCGGA
NotI 67 GCGGCCGC GC^GGCCGC
PshAI 3206 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3320 ATTAAT AT^TAAT
SapI 2057 GCTCTTC GCTCTTC(1/4)
SbfI 234 CCTGCAGG CCTGCA^GG
SphI 2509 GCATGC GCATG^C
Sse8387I 234 CCTGCAGG CCTGCA^GG
SspBI 2368 TGTACA T^GTACA
SwaI 2898 ATTTAAAT ATTT^AAAT
TaqI 209 TCGA T^CGA
TthHB8I 209 TCGA T^CGA
XcmI 2272 CCATCTTCCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Alw44I 2353 GTGCAC G^TGCAC
4003 GTGCAC
ApaLI 2353 GTGCAC G^TGCAC
4003 GTGCAC
Asp700I 2396 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3919 GAAACTCTTC
AvaI 95 CCCGGG C^[CT]CG[GA]G
194 CCCGGG
BalI 1153 TGGCCA TGG^CCA
4766 TGGCCA
BamHI 252 GGATCC G^GATCC
1621 GGATCC
BanI 138 GGTGCC G^G[CT][GA]CC
288 GGTGCC
BcgI 266 GCATCACGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1036 GCAGATATGTCG
BglII 2517 AGATCT A^GATCT
2623 AGATCT
BsmBI 4078 CGTCTC CGTCTC(1/5)
933 GAGACG
BsoBI 95 CCCGGG C^[CT]CG[GA]G
194 CCCGGG
BspMI 233 ACCTGC ACCTGC(4/8)
1363 ACCTGC
BssSI 2340 CACGAG CACGAG(-5/-1)
4270 CTCGTG
Bst1107I 1440 GTATAC GTA^TAC
3153 GTATAC
BstZ17I 1440 GTATAC GTA^TAC
3153 GTATAC
DraII 122 GGGTCCT [GA]G^G[ACGT]CC[CT]
2734 GGGTCCT
EcoNI 104 CCTGTAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1612 CCTTGAGAAGG
EcoO109I 122 GGGTCCT [GA]G^G[ACGT]CC[CT]
2734 GGGTCCT
EcoT22I 2511 ATGCAT ATGCA^T
4967 ATGCAT
FauI 63 GCGGG CCCGC(4/6)
278 GCGGG
HincII 962 GTCAAC GT[CT]^[GA]AC
4739 GTTAAC
HindII 962 GTCAAC GT[CT]^[GA]AC
4739 GTTAAC
HindIII 3836 AAGCTT A^AGCTT
4313 AAGCTT
MluNI 1153 TGGCCA TGG^CCA
4766 TGGCCA
MscI 1153 TGGCCA TGG^CCA
4766 TGGCCA
NsiI 2511 ATGCAT ATGCA^T
4967 ATGCAT
PflMI 3928 CCAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4276 CCACTTGATGG
PpuMI 122 GGGTCCT [GA]G^G[AT]CC[CT]
2734 GGGTCCT
SmaI 95 CCCGGG CCC^GGG
194 CCCGGG
Van91I 3928 CCAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4276 CCACTTGATGG
XmaCI 95 CCCGGG C^CCGGG
194 CCCGGG
XmaI 95 CCCGGG C^CCGGG
194 CCCGGG
XmnI 2396 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3919 GAAACTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaII BanII BbsI BbvI
BciVI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspLU11I
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI EaeI EarI
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SpeI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII