################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AatII Acc65I AclI AcyI AgeI AhdI AocI Aor51HI ApaI AscI Asp718I AspEI AspI AviII AvrII BbeI BbrPI BbvCI BclI BglI BlnI BlpI Bpu1102I BsaHI BsiWI BspDI BspHI BsrBI BssHII BstBI BstEII BstPI Bsu36I CelII ClaI CpoI DrdI Eam1105I Eco47III Eco81I EcoO65I FbaI FseI FspI Hin1I KasI KpnI KspI MfeI MluI MunI NaeI NarI NcoI NgoMIV NheI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI StuI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccIII 447 TCCGGA T^CCGGA AflII 3047 CTTAAG C^TTAAG AseI 3320 ATTAAT AT^TAAT AsnI 3320 ATTAAT AT^TAAT BfrI 3047 CTTAAG C^TTAAG BsaAI 693 CACGTA [CT]AC^GT[GA] BsaBI 3553 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1256 GAGACC GGTCTC(1/5) BsaWI 447 TCCGGA [AT]^CCGG[AT] BseAI 447 TCCGGA T^CCGGA BsgI 2619 GTGCAG GTGCAG(16/14) BsiEI 68 CGGCCG CG[GA][CT]^CG BsmI 2091 GCATTC GAATGC(1/-1) Bsp1407I 2368 TGTACA T^GTACA BspEI 447 TCCGGA T^CCGGA BsrFI 1750 GCCGGT [GA]^CCGG[CT] BsrGI 2368 TGTACA T^GTACA BstAPI 4204 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 4807 CCATTTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 1750 GCCGGT [GA]^CCGG[CT] DraIII 4845 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 173 CCGTGG C^C[GA][CT]GG EagI 68 CGGCCG C^GGCCG EclXI 68 CGGCCG C^GGCCG Eco52I 68 CGGCCG C^GGCCG EcoRV 4510 GATATC GAT^ATC HaeII 240 GGCGCT [GA]GCGC^[CT] HgaI 1583 GCGTC GACGC(5/10) HpaI 4739 GTTAAC GTT^AAC MamI 3553 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 447 TCCGGA T^CCGGA NotI 67 GCGGCCGC GC^GGCCGC PshAI 3206 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3320 ATTAAT AT^TAAT SapI 2057 GCTCTTC GCTCTTC(1/4) SbfI 234 CCTGCAGG CCTGCA^GG SphI 2509 GCATGC GCATG^C Sse8387I 234 CCTGCAGG CCTGCA^GG SspBI 2368 TGTACA T^GTACA SwaI 2898 ATTTAAAT ATTT^AAAT TaqI 209 TCGA T^CGA TthHB8I 209 TCGA T^CGA XcmI 2272 CCATCTTCCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### Alw44I 2353 GTGCAC G^TGCAC 4003 GTGCAC ApaLI 2353 GTGCAC G^TGCAC 4003 GTGCAC Asp700I 2396 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3919 GAAACTCTTC AvaI 95 CCCGGG C^[CT]CG[GA]G 194 CCCGGG BalI 1153 TGGCCA TGG^CCA 4766 TGGCCA BamHI 252 GGATCC G^GATCC 1621 GGATCC BanI 138 GGTGCC G^G[CT][GA]CC 288 GGTGCC BcgI 266 GCATCACGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1036 GCAGATATGTCG BglII 2517 AGATCT A^GATCT 2623 AGATCT BsmBI 4078 CGTCTC CGTCTC(1/5) 933 GAGACG BsoBI 95 CCCGGG C^[CT]CG[GA]G 194 CCCGGG BspMI 233 ACCTGC ACCTGC(4/8) 1363 ACCTGC BssSI 2340 CACGAG CACGAG(-5/-1) 4270 CTCGTG Bst1107I 1440 GTATAC GTA^TAC 3153 GTATAC BstZ17I 1440 GTATAC GTA^TAC 3153 GTATAC DraII 122 GGGTCCT [GA]G^G[ACGT]CC[CT] 2734 GGGTCCT EcoNI 104 CCTGTAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1612 CCTTGAGAAGG EcoO109I 122 GGGTCCT [GA]G^G[ACGT]CC[CT] 2734 GGGTCCT EcoT22I 2511 ATGCAT ATGCA^T 4967 ATGCAT FauI 63 GCGGG CCCGC(4/6) 278 GCGGG HincII 962 GTCAAC GT[CT]^[GA]AC 4739 GTTAAC HindII 962 GTCAAC GT[CT]^[GA]AC 4739 GTTAAC HindIII 3836 AAGCTT A^AGCTT 4313 AAGCTT MluNI 1153 TGGCCA TGG^CCA 4766 TGGCCA MscI 1153 TGGCCA TGG^CCA 4766 TGGCCA NsiI 2511 ATGCAT ATGCA^T 4967 ATGCAT PflMI 3928 CCAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4276 CCACTTGATGG PpuMI 122 GGGTCCT [GA]G^G[AT]CC[CT] 2734 GGGTCCT SmaI 95 CCCGGG CCC^GGG 194 CCCGGG Van91I 3928 CCAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4276 CCACTTGATGG XmaCI 95 CCCGGG C^CCGGG 194 CCCGGG XmaI 95 CCCGGG C^CCGGG 194 CCCGGG XmnI 2396 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3919 GAAACTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaII BanII BbsI BbvI BciVI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspLU11I BsrDI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EaeI EarI EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SpeI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII